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Conference Paper: Cyclooxygenase-2 is an essential mediator of gonadotropins-induced apoptosis of human ovarian surface epithelial cells

TitleCyclooxygenase-2 is an essential mediator of gonadotropins-induced apoptosis of human ovarian surface epithelial cells
Authors
Issue Date2006
PublisherHong Kong Academy of Medicine
Citation
The 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 69 Abstract no. O53 How to Cite?
AbstractObjective: To investigate the signaling mechanism underlying gonadotropins-mediated apoptosis of human ovarian surface epithelial (OSE) cells. Methods: OSE cells were treated with surge concentrations of gonadotropins (FSH and LH/hCG), membrane, cytosolic, and nuclear fractions were prepared by differential centrifugation. Expression of N-cadherin, b-catenin, and cyclooxygnease-2 (COX-2) were examined by immunoblot analysis. b-catenin/T-cell factor (TCF) transcriptional activity was measured in a luiferase reporter gene assay. Results: We demonstrated that disruption of N-cadherin-mediated cell-cell adhesion by gonadotropins is an important molecular event in the apoptosis in human OSE. This downregulation of N-cadherin was correlated with a redistribution of b-catenin from the plasma membrane to the nucleus, resulting in the activation of b-catenin/T Cell Factor (TCF) transcription. These events were mediated via a phosphatidylinositol 3-kinase (PI3K)-dependent phosphorylation of the inhibitory serine 9 residue of glycogen synthase kinase-3b (GSK3-b). In addition, we showed that COX-2, a target of b-catenin/TCF, is an important mediator of the gonadotropins-induced apoptosis of OSE. Conclusion: These findings shed new light on the molecular mechanism underlying OSE survival and death, a process that is anticipated to have important implication in the ovulation and ovarian cancer risk.
Persistent Identifierhttp://hdl.handle.net/10722/110452
ISSN
2015 Impact Factor: 0.887
2015 SCImago Journal Rankings: 0.279

 

DC FieldValueLanguage
dc.contributor.authorPon, YLen_HK
dc.contributor.authorWong, ASTen_HK
dc.date.accessioned2010-09-26T02:06:32Z-
dc.date.available2010-09-26T02:06:32Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 69 Abstract no. O53-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/110452-
dc.description.abstractObjective: To investigate the signaling mechanism underlying gonadotropins-mediated apoptosis of human ovarian surface epithelial (OSE) cells. Methods: OSE cells were treated with surge concentrations of gonadotropins (FSH and LH/hCG), membrane, cytosolic, and nuclear fractions were prepared by differential centrifugation. Expression of N-cadherin, b-catenin, and cyclooxygnease-2 (COX-2) were examined by immunoblot analysis. b-catenin/T-cell factor (TCF) transcriptional activity was measured in a luiferase reporter gene assay. Results: We demonstrated that disruption of N-cadherin-mediated cell-cell adhesion by gonadotropins is an important molecular event in the apoptosis in human OSE. This downregulation of N-cadherin was correlated with a redistribution of b-catenin from the plasma membrane to the nucleus, resulting in the activation of b-catenin/T Cell Factor (TCF) transcription. These events were mediated via a phosphatidylinositol 3-kinase (PI3K)-dependent phosphorylation of the inhibitory serine 9 residue of glycogen synthase kinase-3b (GSK3-b). In addition, we showed that COX-2, a target of b-catenin/TCF, is an important mediator of the gonadotropins-induced apoptosis of OSE. Conclusion: These findings shed new light on the molecular mechanism underlying OSE survival and death, a process that is anticipated to have important implication in the ovulation and ovarian cancer risk.-
dc.languageengen_HK
dc.publisherHong Kong Academy of Medicine-
dc.relation.ispartofHong Kong Medical Journalen_HK
dc.titleCyclooxygenase-2 is an essential mediator of gonadotropins-induced apoptosis of human ovarian surface epithelial cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailWong, AST: awong1@hkucc.hku.hken_HK
dc.identifier.authorityWong, AST=rp00805en_HK
dc.identifier.hkuros130206en_HK

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