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Conference Paper: The role of glucose regulated protein (GRP)-78 in regulating cell growth and apoptosis in human breast and ovarian cancer cells

TitleThe role of glucose regulated protein (GRP)-78 in regulating cell growth and apoptosis in human breast and ovarian cancer cells
Authors
Issue Date2006
PublisherHong Kong Academy of Medicine
Citation
The 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 169 Abstract no. P210 How to Cite?
AbstractObjective: To investigate the role of GRP78 in regulating cell growth and apoptosis in human breast and ovarian cancer cells and examine whether overexpression of GRP78 protects cells from endoplasmic reticulum (ER) stress and chemotherapeutic drug induced apoptosis. Methods: GRP78 expression was measured by Western blot and immunofluorescent microscopy. Cell cycle analyses and cell proliferation were examined by flow cytometry and MTT assay, respectively. Apoptosis was determined by DAPI and TUNEL staining. Results: Overexpression of GRP78 in both breast and ovarian cancer cells led to increases in cell growth, which was due to decreased apoptosis. Treatment with tunicamycin and 2-deoxyglucose induced an increase in GRP78 expression, suggesting that GRP78 overexpression may alleviate unfolded protein stress in the ER. We also showed that overexpression of GRP78 protected both breast and ovarian cancer cells against apoptosis induced by chemotherapeutic drugs such as paclitaxel. Conclusions: Upregulation of GRP78 is one of the mechanisms which may enhance survival and drug resistance of breast and ovarian cancer cells.
Persistent Identifierhttp://hdl.handle.net/10722/110382
ISSN
2015 Impact Factor: 0.887
2015 SCImago Journal Rankings: 0.279

 

DC FieldValueLanguage
dc.contributor.authorKwan, WYen_HK
dc.contributor.authorYeung, HYen_HK
dc.contributor.authorWong, ASTen_HK
dc.date.accessioned2010-09-26T02:03:31Z-
dc.date.available2010-09-26T02:03:31Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 169 Abstract no. P210-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/110382-
dc.description.abstractObjective: To investigate the role of GRP78 in regulating cell growth and apoptosis in human breast and ovarian cancer cells and examine whether overexpression of GRP78 protects cells from endoplasmic reticulum (ER) stress and chemotherapeutic drug induced apoptosis. Methods: GRP78 expression was measured by Western blot and immunofluorescent microscopy. Cell cycle analyses and cell proliferation were examined by flow cytometry and MTT assay, respectively. Apoptosis was determined by DAPI and TUNEL staining. Results: Overexpression of GRP78 in both breast and ovarian cancer cells led to increases in cell growth, which was due to decreased apoptosis. Treatment with tunicamycin and 2-deoxyglucose induced an increase in GRP78 expression, suggesting that GRP78 overexpression may alleviate unfolded protein stress in the ER. We also showed that overexpression of GRP78 protected both breast and ovarian cancer cells against apoptosis induced by chemotherapeutic drugs such as paclitaxel. Conclusions: Upregulation of GRP78 is one of the mechanisms which may enhance survival and drug resistance of breast and ovarian cancer cells.-
dc.languageengen_HK
dc.publisherHong Kong Academy of Medicine-
dc.relation.ispartofHong Kong Medical Journalen_HK
dc.titleThe role of glucose regulated protein (GRP)-78 in regulating cell growth and apoptosis in human breast and ovarian cancer cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailYeung, HY: bhyyeung@gmail.comen_HK
dc.identifier.emailWong, AST: awong1@hkucc.hku.hken_HK
dc.identifier.authorityWong, AST=rp00805en_HK
dc.identifier.hkuros130207en_HK

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