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Conference Paper: Bivariate flow cytometric analysis of hl-60 cell cycle progression affected by polysaccharopeptide (psp) from coriolus versicolor mycelia

TitleBivariate flow cytometric analysis of hl-60 cell cycle progression affected by polysaccharopeptide (psp) from coriolus versicolor mycelia
Authors
Issue Date2004
PublisherWiley
Citation
ISAC XXII International Congress, Montpellier, France, 22-27 May 2004. In Cytometry, 2004, v. 59A n. 1, p. 118-119 How to Cite?
AbstractIntroduction: Anticancer agents generally target on cancer cells either by alter-ation of their cell cycle or induction of apoptosis or necrosis. The polysaccha-ropeptide (PSP)(I’m-Yunity™) isolated from the fermented mycelia of fungusCoriolus versicolor Cov-1™ strain exerts potent activity on inhibition of cell pro-liferation in a wide range of malignancies. However, whether the anticancermechanism(s) of PSP involves alteration of cell cycle is still not clear. The spe-cific aim of this study was to apply 5-bromodeoxyuridine (BrdUrd) to pulse labelactive synthesizing DNA to investigate the effect of PSP on cell cycle kinetics onhuman promyelocytic leukemic HL-60 cells. Methods: HL-60 cells were pulse-labeled with BrdUrd, treated with and without PSP (25, 400 _g/ml), and sampledat certain time intervals. The cell cycle kinetics was studied by usingBrdUrd/Propidium iodide (PI) bivariate flow cytometric analysis. Results: Theresults revealed that high dose of PSP reduced cells in G0/G1 phase, accumulat-ed cells in both S and G2/M phase. The DNA synthesis time and cancer potentialdoubling time was significantly extended in the HL-60 cells after PSP treatment.Conclusion: PSP retards cancer growth via elongation on the S phase, slows thecells returning to G1 from G2/M. Our data indicate that the interference on cellcycle progression is part of the anticancer mechanism(s) of PSP.
Persistent Identifierhttp://hdl.handle.net/10722/110282
ISSN
2004 Impact Factor: 2.698
2007 SCImago Journal Rankings: 0.190

 

DC FieldValueLanguage
dc.contributor.authorWan, JMFen_HK
dc.contributor.authorYang, Xen_HK
dc.contributor.authorSit, WHen_HK
dc.date.accessioned2010-09-26T01:59:09Z-
dc.date.available2010-09-26T01:59:09Z-
dc.date.issued2004en_HK
dc.identifier.citationISAC XXII International Congress, Montpellier, France, 22-27 May 2004. In Cytometry, 2004, v. 59A n. 1, p. 118-119-
dc.identifier.issn0196-4763-
dc.identifier.urihttp://hdl.handle.net/10722/110282-
dc.description.abstractIntroduction: Anticancer agents generally target on cancer cells either by alter-ation of their cell cycle or induction of apoptosis or necrosis. The polysaccha-ropeptide (PSP)(I’m-Yunity™) isolated from the fermented mycelia of fungusCoriolus versicolor Cov-1™ strain exerts potent activity on inhibition of cell pro-liferation in a wide range of malignancies. However, whether the anticancermechanism(s) of PSP involves alteration of cell cycle is still not clear. The spe-cific aim of this study was to apply 5-bromodeoxyuridine (BrdUrd) to pulse labelactive synthesizing DNA to investigate the effect of PSP on cell cycle kinetics onhuman promyelocytic leukemic HL-60 cells. Methods: HL-60 cells were pulse-labeled with BrdUrd, treated with and without PSP (25, 400 _g/ml), and sampledat certain time intervals. The cell cycle kinetics was studied by usingBrdUrd/Propidium iodide (PI) bivariate flow cytometric analysis. Results: Theresults revealed that high dose of PSP reduced cells in G0/G1 phase, accumulat-ed cells in both S and G2/M phase. The DNA synthesis time and cancer potentialdoubling time was significantly extended in the HL-60 cells after PSP treatment.Conclusion: PSP retards cancer growth via elongation on the S phase, slows thecells returning to G1 from G2/M. Our data indicate that the interference on cellcycle progression is part of the anticancer mechanism(s) of PSP.-
dc.languageengen_HK
dc.publisherWiley-
dc.relation.ispartofCytometryen_HK
dc.titleBivariate flow cytometric analysis of hl-60 cell cycle progression affected by polysaccharopeptide (psp) from coriolus versicolor myceliaen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailSit, WH: whsit@HKUCC.hku.hken_HK
dc.identifier.emailWan, JMF: jmfwan@hkusua.hku.hken_HK
dc.identifier.authorityWan, JMF=rp00798en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/cyto.a.20046.abs-
dc.identifier.hkuros104588en_HK

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