File Download
There are no files associated with this item.
Supplementary
-
Citations:
- Appears in Collections:
Conference Paper: Cloning of Chicken Pre-B-cell Colony-Enhancing Factor 1 (PBEF1) and Characterization of its Spatial Expression
Title | Cloning of Chicken Pre-B-cell Colony-Enhancing Factor 1 (PBEF1) and Characterization of its Spatial Expression |
---|---|
Authors | |
Issue Date | 2005 |
Publisher | Poultry Science Association Inc. The Journal's web site is located at http://ps.fass.org |
Citation | 2005 Poultry Science Association Annual Meeting, Auburn University, Auburn, Alabama, USA, 31 July – 3 August 2005. In Poultry Science, 2005, v. 84 n. Suppl. 1, p. 74, abstract no. 174 How to Cite? |
Abstract | The pre-B-cell colony-enhancing factor 1 (PBEF1) was originally identified from lymphocytes in human as a cytokine to enhance the pre-B-cell colony formation. However, most recent studies demonstrated that it could also mimic effects of insulin and lower plasma glucose level in mice. Using chicken as the
experiment model to explore its potential physiological roles in lower vertebrates, we cloned the full-length cDNA coding for PBEF1 from chicken ovary. It encodes for a 493 amino acid peptide and shares extremely high homology (~94%) with that of mouse, rat and human. Interestingly, unlike most of the
cytokines identified, the putative chicken PBEF1 lacks a typical signal peptide sequence at its N-terminus, thereby raising an interesting issue whether Chicken PBEF1 represents a secreted cytokine or a cytosolic enzyme as reported in rats and mice. Using RT-PCR and Northern blot analysis, we noticed that chicken PBEF1 was abundantly expressed in all of the examined tissues including brain, heart, lung, liver, intestine, kidney, muscle, ovary, pancreas, testis, pituitary and spleen. The ubiquitous expression of chicken PBEF1, together with its conserved amino acid sequence, clearly implies that PBEF1 might play critical role in a variety of tissues besides lymphoid tissue and fatty tissues found in vertebrates. |
Persistent Identifier | http://hdl.handle.net/10722/110268 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 1.080 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, J | - |
dc.contributor.author | Wang, Y | - |
dc.contributor.author | Leung, FCC | - |
dc.date.accessioned | 2010-09-26T01:58:32Z | - |
dc.date.available | 2010-09-26T01:58:32Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | 2005 Poultry Science Association Annual Meeting, Auburn University, Auburn, Alabama, USA, 31 July – 3 August 2005. In Poultry Science, 2005, v. 84 n. Suppl. 1, p. 74, abstract no. 174 | - |
dc.identifier.issn | 0032-5791 | - |
dc.identifier.uri | http://hdl.handle.net/10722/110268 | - |
dc.description.abstract | The pre-B-cell colony-enhancing factor 1 (PBEF1) was originally identified from lymphocytes in human as a cytokine to enhance the pre-B-cell colony formation. However, most recent studies demonstrated that it could also mimic effects of insulin and lower plasma glucose level in mice. Using chicken as the experiment model to explore its potential physiological roles in lower vertebrates, we cloned the full-length cDNA coding for PBEF1 from chicken ovary. It encodes for a 493 amino acid peptide and shares extremely high homology (~94%) with that of mouse, rat and human. Interestingly, unlike most of the cytokines identified, the putative chicken PBEF1 lacks a typical signal peptide sequence at its N-terminus, thereby raising an interesting issue whether Chicken PBEF1 represents a secreted cytokine or a cytosolic enzyme as reported in rats and mice. Using RT-PCR and Northern blot analysis, we noticed that chicken PBEF1 was abundantly expressed in all of the examined tissues including brain, heart, lung, liver, intestine, kidney, muscle, ovary, pancreas, testis, pituitary and spleen. The ubiquitous expression of chicken PBEF1, together with its conserved amino acid sequence, clearly implies that PBEF1 might play critical role in a variety of tissues besides lymphoid tissue and fatty tissues found in vertebrates. | - |
dc.language | eng | - |
dc.publisher | Poultry Science Association Inc. The Journal's web site is located at http://ps.fass.org | - |
dc.relation.ispartof | Poultry Science | - |
dc.relation.ispartof | 2005 Poultry Science Association Annual Meeting | - |
dc.title | Cloning of Chicken Pre-B-cell Colony-Enhancing Factor 1 (PBEF1) and Characterization of its Spatial Expression | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Li, J: jlia@graduate.hku.hk | - |
dc.identifier.email | Leung, FCC: fcleung@hkucc.hku.hk | - |
dc.identifier.authority | Leung, FCC=rp00731 | en_HK |
dc.identifier.hkuros | 123622 | - |
dc.identifier.volume | 84 | - |
dc.identifier.issue | Suppl. 1 | - |
dc.identifier.spage | 74 | - |
dc.identifier.epage | 74 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0032-5791 | - |