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Conference Paper: Osmotic Response Element Binding Protein is essential for water reabsorption in kidney

TitleOsmotic Response Element Binding Protein is essential for water reabsorption in kidney
Authors
Issue Date2004
PublisherFederation of American Societies for Experimental Biology
Citation
Experimental Biology 2004, Washington, DC, 17-21 April 2004. In The FASEB Journal, 2004, v. 18 n. 4-5, Abstract no. 673.26 How to Cite?
AbstractIn mammalian kidney, the osmolarity of the inner medulla is highly hypertonic. The cells protect themselves from shrinkage by the expression of a number of genes, such as AR and SMIT that increase the cellular levels of compatible osmolytes. The transcription of these genes is regulated by OREBP. Two independent lines of transgenic (Tg ) mice that overexpress the dominant-negative form of OREBP specifically in the collecting ducts were generated to study the physiological role of OREBP in the kidney. These Tg mice exhibited polyuria, polydipsia due to impaired urine concentrating ability. There was a reduction in the mRNA expression level of AQP-2, UTA-1 and UTA-2 in both renal medulla and renal epithelial cells of Tg mice but no change in the AQP-3 expression. However, during dehydration, Tg mice were able to concentrate their urine to ~30% of that in the non-Tg mice and there was up-regulation of AQP-2 and UTA-1 mRNA expression. Our data suggest that under normal physiological condition, OREBP plays an important role in water reabsorption in the kidney by regulating the expression of AQP-2, UTA-1 and UTA-2. Under dehydrated condition, increase of water reabsorption by the vasopressin/AQP2 system remains functional in the absence of OREBP. Furthermore, the Tg mice developed bilateral hydronephrosis progressively, suggesting that OREBP may also protect the renal cells against osmotic stress.
Persistent Identifierhttp://hdl.handle.net/10722/108833
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorLam, AKMen_HK
dc.contributor.authorKo, CBen_HK
dc.contributor.authorTam, Sen_HK
dc.contributor.authorKnepper, MAen_HK
dc.contributor.authorMorris, Ren_HK
dc.contributor.authorYang, JYen_HK
dc.contributor.authorChung, SKen_HK
dc.contributor.authorChung, SSMen_HK
dc.date.accessioned2010-09-26T00:56:25Z-
dc.date.available2010-09-26T00:56:25Z-
dc.date.issued2004en_HK
dc.identifier.citationExperimental Biology 2004, Washington, DC, 17-21 April 2004. In The FASEB Journal, 2004, v. 18 n. 4-5, Abstract no. 673.26-
dc.identifier.issn0892-6638-
dc.identifier.urihttp://hdl.handle.net/10722/108833-
dc.description.abstractIn mammalian kidney, the osmolarity of the inner medulla is highly hypertonic. The cells protect themselves from shrinkage by the expression of a number of genes, such as AR and SMIT that increase the cellular levels of compatible osmolytes. The transcription of these genes is regulated by OREBP. Two independent lines of transgenic (Tg ) mice that overexpress the dominant-negative form of OREBP specifically in the collecting ducts were generated to study the physiological role of OREBP in the kidney. These Tg mice exhibited polyuria, polydipsia due to impaired urine concentrating ability. There was a reduction in the mRNA expression level of AQP-2, UTA-1 and UTA-2 in both renal medulla and renal epithelial cells of Tg mice but no change in the AQP-3 expression. However, during dehydration, Tg mice were able to concentrate their urine to ~30% of that in the non-Tg mice and there was up-regulation of AQP-2 and UTA-1 mRNA expression. Our data suggest that under normal physiological condition, OREBP plays an important role in water reabsorption in the kidney by regulating the expression of AQP-2, UTA-1 and UTA-2. Under dehydrated condition, increase of water reabsorption by the vasopressin/AQP2 system remains functional in the absence of OREBP. Furthermore, the Tg mice developed bilateral hydronephrosis progressively, suggesting that OREBP may also protect the renal cells against osmotic stress.-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology-
dc.relation.ispartofThe FASEB Journalen_HK
dc.titleOsmotic Response Element Binding Protein is essential for water reabsorption in kidneyen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLam, AKM: akmlam@hkucc.hku.hken_HK
dc.identifier.emailKo, CB: cbko@hkucc.hku.hken_HK
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_HK
dc.identifier.emailChung, SSM: smchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.identifier.authorityChung, SSM=rp00376en_HK
dc.identifier.hkuros91796en_HK

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