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Conference Paper: Viral specific T cells phenotypes and toll like receptors mRNA expression in CD8 cells of chronic hepatitis B patients

TitleViral specific T cells phenotypes and toll like receptors mRNA expression in CD8 cells of chronic hepatitis B patients
Authors
Issue Date2006
PublisherBlackwell Publishing Asia.
Citation
Shanghai—Hong Kong International Liver Congress, Shanghai, China, 25–28 March 2006. In Journal of Gastroenterology and Hepatology, 2006, v. 21 n. S2, p. A136 How to Cite?
AbstractBackground The clearance or persistence of hepatitis B virus(HBV) is affected by HBV-specific cytotoxic T-lymphocyte (CTL).Since toll like receptors (TLRs) may contribute to the viral specificT cells. But what and how the TLRs take their functions in chronichepatitis B (CHB) patients remains unknown.Aims To investigate the association of TLRs in CD8+ T cells andtheir relationship with CTL cells in chronic hepatitis B patients.Material and methods Peripheral blood mononuclear cells(PBMC) were collected from 28 HLA-A2+ patients. Group A (n =8) were patients with spontaneous resolution of CHB (anti-HBc plusanti-HBs positive) while Group B (n = 20) was HBeAg (+) CHBpatients. Ex-vivo model:The PBMC of these patients were co-culturedwith live hepatitis B virus (HBV) for 7 days. PBMC were stained forthe frequency of CD4, CD8, CD45RA, CD45RO and CCR7 byFACS analysis. Viral specific CD8 cells were tested by tetramer toHLA-A2 limited HBV peptides. CD8+ cells were isolated and TLRs-2, 3 4 and 7 mRNA level in CD8+ cells and HBV DNA were testedby real time PCR. Interferon-g secretion was tested by ELISpot.Results After co-culture, there was an increase in HBV-specificCD8+tetramer+ cells in Group A when compared with Group B(mean ± SD 1.971 ± 0.694% vs. 0.7 ± 0.362%, respectively, P 0.001).Interferon-γ secretion was higher in Group A when compared with ingroup B on day 7 after stimulation (SFC were 304.25 ± 156.12 inGroup A and 49.6 ± 46.04 in Group B, P = 0.0002).The HBV DNAin the ex-vivo model was lower in Group A from day-5 to Day-7 ofco-culture. The frequency of CD8+CD45RA-CCR7-cells before co-culture was similar in both groups In both groups, 90% of the HBV-specific CD8+ cells were CD45RA-CCR7- effecter-memory T cells.TLR2, 3 and 4 mRNA were higher expressed in Group A than inGroup B. But only TLR4 has statistic significance (mean ± SD 2.915± 1.536 in Group A vs. 1.010 ± 0.988 in Group B respectively, p0.006).Conclusion HBV-specific CD8+tetramer+ cells are higher inpatients with spontaneous resolution of chronic HBV. TLR4 may beassociated with activation T memory cell in response to HBV.There were no significant differences among the phenotypes ofCD8/tetramer positive cells between the two groups.
Persistent Identifierhttp://hdl.handle.net/10722/108467
ISSN
2015 Impact Factor: 3.322
2015 SCImago Journal Rankings: 1.190

 

DC FieldValueLanguage
dc.contributor.authorZhang, HYen_HK
dc.contributor.authorHui, CKen_HK
dc.contributor.authorLee, PYen_HK
dc.contributor.authorYueng, YHen_HK
dc.contributor.authorLuk, JMCen_HK
dc.contributor.authorLau, Gen_HK
dc.date.accessioned2010-09-26T00:40:52Z-
dc.date.available2010-09-26T00:40:52Z-
dc.date.issued2006en_HK
dc.identifier.citationShanghai—Hong Kong International Liver Congress, Shanghai, China, 25–28 March 2006. In Journal of Gastroenterology and Hepatology, 2006, v. 21 n. S2, p. A136en_HK
dc.identifier.issn0815-9319en_HK
dc.identifier.urihttp://hdl.handle.net/10722/108467-
dc.description.abstractBackground The clearance or persistence of hepatitis B virus(HBV) is affected by HBV-specific cytotoxic T-lymphocyte (CTL).Since toll like receptors (TLRs) may contribute to the viral specificT cells. But what and how the TLRs take their functions in chronichepatitis B (CHB) patients remains unknown.Aims To investigate the association of TLRs in CD8+ T cells andtheir relationship with CTL cells in chronic hepatitis B patients.Material and methods Peripheral blood mononuclear cells(PBMC) were collected from 28 HLA-A2+ patients. Group A (n =8) were patients with spontaneous resolution of CHB (anti-HBc plusanti-HBs positive) while Group B (n = 20) was HBeAg (+) CHBpatients. Ex-vivo model:The PBMC of these patients were co-culturedwith live hepatitis B virus (HBV) for 7 days. PBMC were stained forthe frequency of CD4, CD8, CD45RA, CD45RO and CCR7 byFACS analysis. Viral specific CD8 cells were tested by tetramer toHLA-A2 limited HBV peptides. CD8+ cells were isolated and TLRs-2, 3 4 and 7 mRNA level in CD8+ cells and HBV DNA were testedby real time PCR. Interferon-g secretion was tested by ELISpot.Results After co-culture, there was an increase in HBV-specificCD8+tetramer+ cells in Group A when compared with Group B(mean ± SD 1.971 ± 0.694% vs. 0.7 ± 0.362%, respectively, P 0.001).Interferon-γ secretion was higher in Group A when compared with ingroup B on day 7 after stimulation (SFC were 304.25 ± 156.12 inGroup A and 49.6 ± 46.04 in Group B, P = 0.0002).The HBV DNAin the ex-vivo model was lower in Group A from day-5 to Day-7 ofco-culture. The frequency of CD8+CD45RA-CCR7-cells before co-culture was similar in both groups In both groups, 90% of the HBV-specific CD8+ cells were CD45RA-CCR7- effecter-memory T cells.TLR2, 3 and 4 mRNA were higher expressed in Group A than inGroup B. But only TLR4 has statistic significance (mean ± SD 2.915± 1.536 in Group A vs. 1.010 ± 0.988 in Group B respectively, p0.006).Conclusion HBV-specific CD8+tetramer+ cells are higher inpatients with spontaneous resolution of chronic HBV. TLR4 may beassociated with activation T memory cell in response to HBV.There were no significant differences among the phenotypes ofCD8/tetramer positive cells between the two groups.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia.en_HK
dc.relation.ispartofJournal of Gastroenterology and Hepatologyen_HK
dc.titleViral specific T cells phenotypes and toll like receptors mRNA expression in CD8 cells of chronic hepatitis B patientsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0815-9319&volume=21 Suppl&spage=A136&epage=A136&date=2006&atitle=Viral+specific+T+cells+phenotypes+and+toll+like+receptors+mRNA+expression+in+CD8+cells+of+chronic+hepatitis+B+patientsen_HK
dc.identifier.emailHui, CK: ckh23@cam.ac.uken_HK
dc.identifier.emailLee, PY: nikkilee@hkucc.hku.hken_HK
dc.identifier.emailYueng, YH: yhyueng@HKUCC-COM.hku.hken_HK
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_HK
dc.identifier.emailLau, G: gkklau@netvigator.comen_HK
dc.identifier.authorityLuk, JMC=rp00349en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1746.2006.04409.x-
dc.identifier.hkuros137533en_HK
dc.identifier.volume21en_HK
dc.identifier.spage136en_HK
dc.identifier.epage136en_HK

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