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Conference Paper: Proteomic expression clustering of mouse liver developmental stages

TitleProteomic expression clustering of mouse liver developmental stages
Authors
Issue Date2006
PublisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JGH
Citation
The 2006 Shanghai-Hong Kong International Liver Congress, Shanghai, China, 25-28 March 2006. In Journal of Gastroenterology and Hepatology, v. 21 suppl. s2, p. A218-A219, abstract no. 493 How to Cite?
AbstractLiver starts to differentiate as early as in gestation day 8 in mouse and continues to develop till adulthood. It undergoes functional transition from acting as a hematopoietic center in embryo to being the meta-bolic center in adult. During this process, it accompanies with alteration in the protein expressions in liver. As such, it is important to delineate the protein expression profiling during mouse liver development from embryo to adult, aiming to provide information for the study of liver functions. In this study, livers were extracted from prenatal, neonatal, and postnatal livers: embryonic day 13.5 and 16.5 (E13.5 and E16.5), newborn liver, and livers from 3 week-old and adult mice. The patterns and expressions of proteins (spots) were visualized using 2-dimensional gel electrophoresis, accompanied by spot analysis using PDQuest software. Among the 804 spots identified, 396 spots demonstrated statistical significance among different stages by one-way ANOVA analysis (P < 0.01). Based on the distinct spot expression, spots were further segregated into five clusters by unsupervised learning algorithm. By linear regression analysis, we also identified protein species that are associated with developmental changes at pre-, neo, and post-natal livers. About 10–15 spots from each cluster were identified using the MALDI-ToF/MS or MS/MS approach. Information derived from the protein spot identification results advance the knowledge in liver development.
DescriptionThis journal suppl. entitled: Abstract and Proceeding of the Shanghai-Hong Kong International Liver Congress
Poster Session - Basic Science Including Genomics and Proteomics
Persistent Identifierhttp://hdl.handle.net/10722/108317
ISSN
2015 Impact Factor: 3.322
2015 SCImago Journal Rankings: 1.190

 

DC FieldValueLanguage
dc.contributor.authorLee, NPYen_HK
dc.contributor.authorLeung, KWen_HK
dc.contributor.authorSham, PCen_HK
dc.contributor.authorLau, GKKen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorLuk, JMCen_HK
dc.date.accessioned2010-09-26T00:34:34Z-
dc.date.available2010-09-26T00:34:34Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 2006 Shanghai-Hong Kong International Liver Congress, Shanghai, China, 25-28 March 2006. In Journal of Gastroenterology and Hepatology, v. 21 suppl. s2, p. A218-A219, abstract no. 493en_HK
dc.identifier.issn0815-9319-
dc.identifier.urihttp://hdl.handle.net/10722/108317-
dc.descriptionThis journal suppl. entitled: Abstract and Proceeding of the Shanghai-Hong Kong International Liver Congress-
dc.descriptionPoster Session - Basic Science Including Genomics and Proteomics-
dc.description.abstractLiver starts to differentiate as early as in gestation day 8 in mouse and continues to develop till adulthood. It undergoes functional transition from acting as a hematopoietic center in embryo to being the meta-bolic center in adult. During this process, it accompanies with alteration in the protein expressions in liver. As such, it is important to delineate the protein expression profiling during mouse liver development from embryo to adult, aiming to provide information for the study of liver functions. In this study, livers were extracted from prenatal, neonatal, and postnatal livers: embryonic day 13.5 and 16.5 (E13.5 and E16.5), newborn liver, and livers from 3 week-old and adult mice. The patterns and expressions of proteins (spots) were visualized using 2-dimensional gel electrophoresis, accompanied by spot analysis using PDQuest software. Among the 804 spots identified, 396 spots demonstrated statistical significance among different stages by one-way ANOVA analysis (P < 0.01). Based on the distinct spot expression, spots were further segregated into five clusters by unsupervised learning algorithm. By linear regression analysis, we also identified protein species that are associated with developmental changes at pre-, neo, and post-natal livers. About 10–15 spots from each cluster were identified using the MALDI-ToF/MS or MS/MS approach. Information derived from the protein spot identification results advance the knowledge in liver development.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JGH-
dc.relation.ispartofJournal of Gastroenterology and Hepatologyen_HK
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.titleProteomic expression clustering of mouse liver developmental stagesen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLee, NPY: nikkilee@hkucc.hku.hken_HK
dc.identifier.emailLeung, KW: leungkan@graduate.hku.hken_HK
dc.identifier.emailSham, PC: pcsham@hku.hken_HK
dc.identifier.emailLau, GKK: gkklau@netvigator.comen_HK
dc.identifier.emailFan, ST: stfan@hku.hk-
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hk-
dc.identifier.authorityLee, NPY=rp00263en_HK
dc.identifier.authoritySham, PC=rp00459en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1746.2006.04408.x-
dc.identifier.hkuros117154en_HK
dc.identifier.hkuros120439-
dc.identifier.volume21en_HK
dc.identifier.issuesuppl. s2en_HK
dc.identifier.spageA218, abstract no. 493en_HK
dc.identifier.epageA219-
dc.publisher.placeAustralia-

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