File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: Chemo-resistant cells contain cancer stem like cells

TitleChemo-resistant cells contain cancer stem like cells
Authors
Issue Date2007
PublisherAmerican Association for Cancer Research
Citation
The 98th AACR Annual Meeting, Los Angeles, CA, 14-18 April 2007. In Cancer Research, 2007, v. 67 n. 9S, p. 1311 How to Cite?
AbstractOne of the hypotheses in cancer stem cell biology is that chemotherapy kills most cancer cells but leave cancer stem cells behind. It is generally accepted that high expression level of specific ABC drug transporters in cancer stem cells contribute to drug resistance. However, purified ABCG2+ cancer cells have very similar or less tumorigenicity to the ABCG2- cells; and whether ABCG2+ or ABCG2- represents more caner stem like cells is yet conclusive. We used anticancer drugs such as doxorubicin, 5-FU, and cisplatin either alone or with combination to treat several cancer cell lines. Using RT-PCR, cell migration, colony forming and Xenograft tumorgenesis assay, we observed cell markers, biological features and xenograft tumorigenesis in those chemo resistant cells. Chemo-resistant Hela and liver cancer cells showed enhanced expression of stem cell like markers Oct4 and ABCG2, as well as liver progenitor cell markers CK19 and CC-6. Epithelial mesenchymal transition (EMT) related molecular markers such as N-cadherin and Notch1-Snail pathway were also increased. Chemo-resistant cancer cells displayed increased cell migration and potential metastatic tendency in Xenograft tumor. We also found that self-renewal ability of chemo-resistant cancer cells had enhanced self-renewal ability by colony forming assay in 0.5% serum containing medium but not in 1% serum containing medium. The present study demonstrated that chemo-resistant cells might have stem cell potential and their enhanced migration might contribute to tumor metastasis and recurrence.
Persistent Identifierhttp://hdl.handle.net/10722/108188
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372

 

DC FieldValueLanguage
dc.contributor.authorWang, Xen_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2010-09-26T00:29:09Z-
dc.date.available2010-09-26T00:29:09Z-
dc.date.issued2007en_HK
dc.identifier.citationThe 98th AACR Annual Meeting, Los Angeles, CA, 14-18 April 2007. In Cancer Research, 2007, v. 67 n. 9S, p. 1311-
dc.identifier.issn0008-5472-
dc.identifier.urihttp://hdl.handle.net/10722/108188-
dc.description.abstractOne of the hypotheses in cancer stem cell biology is that chemotherapy kills most cancer cells but leave cancer stem cells behind. It is generally accepted that high expression level of specific ABC drug transporters in cancer stem cells contribute to drug resistance. However, purified ABCG2+ cancer cells have very similar or less tumorigenicity to the ABCG2- cells; and whether ABCG2+ or ABCG2- represents more caner stem like cells is yet conclusive. We used anticancer drugs such as doxorubicin, 5-FU, and cisplatin either alone or with combination to treat several cancer cell lines. Using RT-PCR, cell migration, colony forming and Xenograft tumorgenesis assay, we observed cell markers, biological features and xenograft tumorigenesis in those chemo resistant cells. Chemo-resistant Hela and liver cancer cells showed enhanced expression of stem cell like markers Oct4 and ABCG2, as well as liver progenitor cell markers CK19 and CC-6. Epithelial mesenchymal transition (EMT) related molecular markers such as N-cadherin and Notch1-Snail pathway were also increased. Chemo-resistant cancer cells displayed increased cell migration and potential metastatic tendency in Xenograft tumor. We also found that self-renewal ability of chemo-resistant cancer cells had enhanced self-renewal ability by colony forming assay in 0.5% serum containing medium but not in 1% serum containing medium. The present study demonstrated that chemo-resistant cells might have stem cell potential and their enhanced migration might contribute to tumor metastasis and recurrence.-
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research-
dc.relation.ispartofCancer Researchen_HK
dc.titleChemo-resistant cells contain cancer stem like cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailWang, X: xqwang@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityWang, X=rp00507en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.hkuros140992en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats