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Conference Paper: Idenifcation of L1CAM as the potential biomarker for HCC metastasis

TitleIdenifcation of L1CAM as the potential biomarker for HCC metastasis
Authors
Issue Date2006
PublisherWiley-Blackwell Publishing Asia
Citation
Shanghai—Hong Kong International Liver Congress, Shanghai, China, 25–28 March 2006. In Journal of Gastroenterology and Hepatology, 2006, v. 21 n. S2, p. A78 How to Cite?
AbstractBackground The poor prognosis of HCC is commonly associatedwith metastasis and recurrence. Recently, two HCC metastatic celllines MHCC-97L and MHCC-97H, with different metastastic poten-tial, were established. A high density human U133 plus 2.0 cDNAmicroarray was used to examine their genetic alterations. Among theeight most up-regulated genes, we identified L1 adhesion molecule(L1CAM) as a potential important protein for HCC metastasis.Methods and Results In this study, we investigated expressionpatterns of L1CAM in human HCC tumor samples and correlationto clinicopathologic variables including patient survival times. Thefunctional role of L1 was also studied in-vitro. Finally, we alsoassessed whether soluble L1CAM detected in serum samples of HCCpatients L1CAM expression is associated significantly with venousinfiltration (p = 0.001), advanced TNM stage (p < 0.001), recurrence(p < 0.001) and microsatellite formation (p = 0.011). By tissuemicroarray, L1 expression was correlated with HCC metastasis (p <0.05). Strong membranous L1 expression correlated significantly withshorter survival and disease-free survival (p = 0.0129 and p = 0.0046respectively). Consistently, L1 expression was only found in metasta-tic HCC cell lines but not the primary ones. By ectopic L1 cDNAtransfection into Hep3B cell, increased metastatic potential was evi-denced by wound healing assay and invasion assay. L1CAM pro-moted both lung and liver metastases after tail vein injection of nudemice. By immunoprecipitation, we could detect ectodomain of L1 inHCC patients’ serum and their expression was significantly elevatedwhen compared with the normal controls.Conclusion L1CAM is a new marker for prediction of clinicaloutcome and could be helpful to identify patients with HCC who areat high risk for recurrent disease.
Persistent Identifierhttp://hdl.handle.net/10722/108104
ISSN
2014 Impact Factor: 3.504
2014 SCImago Journal Rankings: 1.128

 

DC FieldValueLanguage
dc.contributor.authorLee, KWen_HK
dc.contributor.authorPoon, RTPen_HK
dc.contributor.authorTang, ZYen_HK
dc.contributor.authorSun, HCen_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2010-09-26T00:25:37Z-
dc.date.available2010-09-26T00:25:37Z-
dc.date.issued2006en_HK
dc.identifier.citationShanghai—Hong Kong International Liver Congress, Shanghai, China, 25–28 March 2006. In Journal of Gastroenterology and Hepatology, 2006, v. 21 n. S2, p. A78en_HK
dc.identifier.issn0815-9319-
dc.identifier.urihttp://hdl.handle.net/10722/108104-
dc.description.abstractBackground The poor prognosis of HCC is commonly associatedwith metastasis and recurrence. Recently, two HCC metastatic celllines MHCC-97L and MHCC-97H, with different metastastic poten-tial, were established. A high density human U133 plus 2.0 cDNAmicroarray was used to examine their genetic alterations. Among theeight most up-regulated genes, we identified L1 adhesion molecule(L1CAM) as a potential important protein for HCC metastasis.Methods and Results In this study, we investigated expressionpatterns of L1CAM in human HCC tumor samples and correlationto clinicopathologic variables including patient survival times. Thefunctional role of L1 was also studied in-vitro. Finally, we alsoassessed whether soluble L1CAM detected in serum samples of HCCpatients L1CAM expression is associated significantly with venousinfiltration (p = 0.001), advanced TNM stage (p < 0.001), recurrence(p < 0.001) and microsatellite formation (p = 0.011). By tissuemicroarray, L1 expression was correlated with HCC metastasis (p <0.05). Strong membranous L1 expression correlated significantly withshorter survival and disease-free survival (p = 0.0129 and p = 0.0046respectively). Consistently, L1 expression was only found in metasta-tic HCC cell lines but not the primary ones. By ectopic L1 cDNAtransfection into Hep3B cell, increased metastatic potential was evi-denced by wound healing assay and invasion assay. L1CAM pro-moted both lung and liver metastases after tail vein injection of nudemice. By immunoprecipitation, we could detect ectodomain of L1 inHCC patients’ serum and their expression was significantly elevatedwhen compared with the normal controls.Conclusion L1CAM is a new marker for prediction of clinicaloutcome and could be helpful to identify patients with HCC who areat high risk for recurrent disease.-
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing Asia-
dc.relation.ispartofJournal of Gastroenterology and Hepatologyen_HK
dc.titleIdenifcation of L1CAM as the potential biomarker for HCC metastasisen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLee, KW: tkwlee@hkucc.hku.hken_HK
dc.identifier.emailPoon, RTP: poontp@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityLee, KW=rp00447en_HK
dc.identifier.authorityPoon, RTP=rp00446en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1746.2006.04402.x-
dc.identifier.hkuros117036en_HK
dc.identifier.volume21en_HK
dc.identifier.issueSuppl 2en_HK
dc.identifier.spage78en_HK

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