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Conference Paper: Liver intestine-cadherin (CDH17) haplotype is associated with alternative mRNA splicing and increased risk of hepatocellular carcinoma

TitleLiver intestine-cadherin (CDH17) haplotype is associated with alternative mRNA splicing and increased risk of hepatocellular carcinoma
Authors
Issue Date2006
PublisherBlackwell Publishing Asia.
Citation
The 2006 Shanghai-Hong Kong International Liver Congress, Shanghai, China, 25-28 March 2006. In Journal of Gastroenterology and Hepatology, v. 21 suppl. S2, p. A82, abstract no. 033 How to Cite?
AbstractBACKGROUND: Hepatocellular carcinoma (HCC) is the most common liver malignancy with high mortality worldwide. Our previous study demonstrated that aberrant mRNA splicing of liver intestine (LI)-cadherin gene CDH17 was associated with tumor dissemination and shorter survival of HCC patients. In this study, we aim to investigate the potential link between CDH 17 haplotypes and alternative splicing in HCC. METHODS: One hundred and sixty-four HCC and 99 cirrhosis patients, and 258 healthy controls were investigated for genetic polymorphisms of CDH17 using PCR and sequencing. Odds ratio and χ2 analysis were used to analyze genotypes and haplotypes. RESULTS: CDH17 651T (C > T) and IVS6 + 35G (A > G) alleles were overrepresented in HCC patients, especially genotypes 651 TT (OR, 2.59; 95% CI, 1.35–4.97) and IVS6 + 35 GG (OR, 1.91; 95% CI, 1.03–3.53) were highly associated with HCC disease. The T-G haplotype was more prevalent in HCC patients compared with healthy controls (OR, 1.57; 95% CI, 1.167–2.109; P = 0.004). The mini-gene assay revealed that the T and G allelic combination could result in exon 7 or both exons 6 and 7 skipping of CDH17 mRNA, indicating that the T-G haplotype might alter the mRNA splicing of LI-cadherin. There was no significant difference in genotype and allele frequencies between the cirrhosis and control groups. However, the allelic frequencies of 651T and IVS6 + 35G significantly increased in a trend of control, cirrhosis toward HCC. CONCLUSION: The functional T-G haplotype of CDH17 (651 C > T and IVS6 + 35 A > G) is a genetic susceptibility factor for the development of HCC in a Chinese population.
DescriptionFree Paper Session: abstract no. 033
Persistent Identifierhttp://hdl.handle.net/10722/108098
ISSN
2015 Impact Factor: 3.322
2015 SCImago Journal Rankings: 1.190

 

DC FieldValueLanguage
dc.contributor.authorWang, XQen_HK
dc.contributor.authorLuk, JMCen_HK
dc.contributor.authorGarcia-Barcelo, MMen_HK
dc.contributor.authorMiao, Xen_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2010-09-26T00:25:22Z-
dc.date.available2010-09-26T00:25:22Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 2006 Shanghai-Hong Kong International Liver Congress, Shanghai, China, 25-28 March 2006. In Journal of Gastroenterology and Hepatology, v. 21 suppl. S2, p. A82, abstract no. 033en_HK
dc.identifier.issn0815-9319en_HK
dc.identifier.urihttp://hdl.handle.net/10722/108098-
dc.descriptionFree Paper Session: abstract no. 033-
dc.description.abstractBACKGROUND: Hepatocellular carcinoma (HCC) is the most common liver malignancy with high mortality worldwide. Our previous study demonstrated that aberrant mRNA splicing of liver intestine (LI)-cadherin gene CDH17 was associated with tumor dissemination and shorter survival of HCC patients. In this study, we aim to investigate the potential link between CDH 17 haplotypes and alternative splicing in HCC. METHODS: One hundred and sixty-four HCC and 99 cirrhosis patients, and 258 healthy controls were investigated for genetic polymorphisms of CDH17 using PCR and sequencing. Odds ratio and χ2 analysis were used to analyze genotypes and haplotypes. RESULTS: CDH17 651T (C > T) and IVS6 + 35G (A > G) alleles were overrepresented in HCC patients, especially genotypes 651 TT (OR, 2.59; 95% CI, 1.35–4.97) and IVS6 + 35 GG (OR, 1.91; 95% CI, 1.03–3.53) were highly associated with HCC disease. The T-G haplotype was more prevalent in HCC patients compared with healthy controls (OR, 1.57; 95% CI, 1.167–2.109; P = 0.004). The mini-gene assay revealed that the T and G allelic combination could result in exon 7 or both exons 6 and 7 skipping of CDH17 mRNA, indicating that the T-G haplotype might alter the mRNA splicing of LI-cadherin. There was no significant difference in genotype and allele frequencies between the cirrhosis and control groups. However, the allelic frequencies of 651T and IVS6 + 35G significantly increased in a trend of control, cirrhosis toward HCC. CONCLUSION: The functional T-G haplotype of CDH17 (651 C > T and IVS6 + 35 A > G) is a genetic susceptibility factor for the development of HCC in a Chinese population.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia.en_HK
dc.relation.ispartofJournal of Gastroenterology and Hepatologyen_HK
dc.titleLiver intestine-cadherin (CDH17) haplotype is associated with alternative mRNA splicing and increased risk of hepatocellular carcinomaen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0815-9319&volume=21 Suppl&spage=A82&epage=A82&date=2006&atitle=Liver+intestine-cadherin+(CDH17)+haplotype+is+associated+with+alternative+mRNA+splicing+and+increased+risk+of+hepatocellular+carcinomaen_HK
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_HK
dc.identifier.emailGarcia-Barcelo, MM: mmgarcia@hkucc.hku.hken_HK
dc.identifier.emailMiao, X: miaoxp@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityLuk, JMC=rp00349en_HK
dc.identifier.authorityGarcia-Barcelo, MM=rp00445en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1746.2006.04403.x-
dc.identifier.hkuros137538en_HK
dc.identifier.volumev. 21en_HK
dc.identifier.issuesuppl. S2-
dc.identifier.spageA82, abstract no. 033en_HK
dc.identifier.epage82en_HK
dc.identifier.epageA82, abstract no. 033-

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