File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Conference Paper: Potential role of resident intrahepatic lymphocytes in adoptive transfer of immunity to hepatitis B virus through liver transplantation

TitlePotential role of resident intrahepatic lymphocytes in adoptive transfer of immunity to hepatitis B virus through liver transplantation
Authors
Issue Date2005
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
Citation
Digestive Disease Week and the 106th Annual Meeting of the American Gastroenterological Association, Chicago, IL., 14-19 May 2005. In Gastroenterology, 2005, v. 128 n. 4 Suppl. 2, A764, Poster no. M1676 How to Cite?
AbstractBackground & Aims: Both murine and human studies have demonstrated the adoptive transfer of hepatitis B virus (HBV)-specific immunity through liver transplantation, which may be attributed to the presence of a significant number of immunocompetent cells in the liver grafts from donors with HBV immunity. In this study, we characterized the resident lymphocytes in human liver grafts, identified the HBV-specific lymphocytes in liver grafts from HBV immune donors, and evaluated the potential role of the intrahepatic lymphocytes in the adoptive transfer of HBV immunity through liver transplantation. Methods: The number, location, and phenotype of resident lymphocytes in human liver grafts were determined by immunohistochemical staining and flow cytometry. The frequency of HBV-specific T cells and B cells was quantified directly by enzyme-linked immunospot (ELISPOT) assay. The serum antibody against hepatitis B surface antigen (anti-HBs) was tested by enzyme immunoassay. Results: The resident lymphocytes were predominantly located in the portal tracts and also scattered throughout the parenchyma of normal liver tissue. Natural Killer (NK) cells (CD 56 ) constituted 27.69.1% of total resident lymphocytes in liver grafts. The majority of intrahepatic CD3 T cells expressed CD8 (67.314.1%), while a smaller proportion expressed CD4 (23.39.7%). NK-T cells bearing both CD3 and CD56 constituted 29.718.1% of intrahepatic CD3 T cells. B cells (CD20 ) accounted for 6.33.8% of the total resident lymphocytes in liver grafts. A significant number of IFN- -secreting T cells specific for HBV antigens were observed in 72.4% (21/29) of liver grafts from donors who are immune to HBV. The anti-HBs-secreting B cells were also detected in 27.6% (8/29) of liver grafts by ELISPOT assay. Spontaneous development of seroconversion to anti-HBs status has been observed in 40.6% (13/32) of patients who underwent liver transplantation for chronic hepatitis B, which was significantly related with the serum anti-HBs level of liver donors and HBsAg-specific T cell number in liver grafts before implantation. Conclusions: Normal liver graft contains a significant number of resident lymphocytes with distinct phenotype. The presence of HBV-specific immunocompetent cells in liver grafts from HBV immune donors may account for the donor-derived immunity to HBV in liver recipients.
Persistent Identifierhttp://hdl.handle.net/10722/108059
ISSN
2015 Impact Factor: 18.187
2015 SCImago Journal Rankings: 7.170

 

DC FieldValueLanguage
dc.contributor.authorLuo, Yen_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorCheung, CKYen_HK
dc.contributor.authorLau, Gen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorWong, Jen_HK
dc.date.accessioned2010-09-26T00:23:44Z-
dc.date.available2010-09-26T00:23:44Z-
dc.date.issued2005en_HK
dc.identifier.citationDigestive Disease Week and the 106th Annual Meeting of the American Gastroenterological Association, Chicago, IL., 14-19 May 2005. In Gastroenterology, 2005, v. 128 n. 4 Suppl. 2, A764, Poster no. M1676-
dc.identifier.issn0016-5085-
dc.identifier.urihttp://hdl.handle.net/10722/108059-
dc.description.abstractBackground & Aims: Both murine and human studies have demonstrated the adoptive transfer of hepatitis B virus (HBV)-specific immunity through liver transplantation, which may be attributed to the presence of a significant number of immunocompetent cells in the liver grafts from donors with HBV immunity. In this study, we characterized the resident lymphocytes in human liver grafts, identified the HBV-specific lymphocytes in liver grafts from HBV immune donors, and evaluated the potential role of the intrahepatic lymphocytes in the adoptive transfer of HBV immunity through liver transplantation. Methods: The number, location, and phenotype of resident lymphocytes in human liver grafts were determined by immunohistochemical staining and flow cytometry. The frequency of HBV-specific T cells and B cells was quantified directly by enzyme-linked immunospot (ELISPOT) assay. The serum antibody against hepatitis B surface antigen (anti-HBs) was tested by enzyme immunoassay. Results: The resident lymphocytes were predominantly located in the portal tracts and also scattered throughout the parenchyma of normal liver tissue. Natural Killer (NK) cells (CD 56 ) constituted 27.69.1% of total resident lymphocytes in liver grafts. The majority of intrahepatic CD3 T cells expressed CD8 (67.314.1%), while a smaller proportion expressed CD4 (23.39.7%). NK-T cells bearing both CD3 and CD56 constituted 29.718.1% of intrahepatic CD3 T cells. B cells (CD20 ) accounted for 6.33.8% of the total resident lymphocytes in liver grafts. A significant number of IFN- -secreting T cells specific for HBV antigens were observed in 72.4% (21/29) of liver grafts from donors who are immune to HBV. The anti-HBs-secreting B cells were also detected in 27.6% (8/29) of liver grafts by ELISPOT assay. Spontaneous development of seroconversion to anti-HBs status has been observed in 40.6% (13/32) of patients who underwent liver transplantation for chronic hepatitis B, which was significantly related with the serum anti-HBs level of liver donors and HBsAg-specific T cell number in liver grafts before implantation. Conclusions: Normal liver graft contains a significant number of resident lymphocytes with distinct phenotype. The presence of HBV-specific immunocompetent cells in liver grafts from HBV immune donors may account for the donor-derived immunity to HBV in liver recipients.-
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro-
dc.relation.ispartofGastroenterologyen_HK
dc.titlePotential role of resident intrahepatic lymphocytes in adoptive transfer of immunity to hepatitis B virus through liver transplantationen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-5085&volume=128&issue=4 Suppl. 2&spage=226&epage=&date=2005&atitle=Potential+role+of+resident+intrahepatic+lymphocytes+in+adoptive+transfer+of+immunity+to+hepatitis+B+virus+through+liver+transplantation-
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.emailCheung, CKY: cindycky@hkucc.hku.hken_HK
dc.identifier.emailLau, G: gkklau@netvigator.comen_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailWong, J: jwong@hkucc.hku.hken_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityWong, J=rp00322en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1053/j.gastro.2005.04.003-
dc.identifier.hkuros99908en_HK
dc.identifier.hkuros120167-
dc.identifier.volume128-
dc.identifier.issue4 Suppl. 2-
dc.identifier.spageA764-
dc.identifier.epage226-
dc.description.otherDigestive Disease Week and the 106th Annual Meeting of the American Gastroenterological Association, Chicago, IL., 14-19 May 2005. In Gastroenterology, 2005, v. 128 n. 4 Suppl. 2, A764, Poster no. M1676-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats