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Conference Paper: A proteomic study of hypoxia stress response on hepatocellular carcinoma in rat orthotopic liver transplantation model

TitleA proteomic study of hypoxia stress response on hepatocellular carcinoma in rat orthotopic liver transplantation model
Authors
Issue Date2006
PublisherBlackwell Publishing Asia.
Citation
Shanghai—Hong Kong International Liver Congress, Shanghai, China, 25–28 March 2006. In Journal of Gastroenterology and Hepatology, 2006, v. 21 n. S2, p. A219 How to Cite?
AbstractHCC is the second most prevalent cancer in Hong Kong and China.However, HCC has a high 3-year recurrence rate at 55% after surgery,which limits the long-term survival of HCC patients. One of theimportant variables in any surgical procedure is the organ ischemictime. Several studies had shown that hypoxic stress is an importantdeterminant in both malignant progression and ischemia-reperfusion.Herein, we postulate that hypoxia will result in proteomic expressionchanges in HCC tumors, thereby enhancing tumor recurrence and/orchemoresistance. Using our established orthotopic liver transplanta-tion model, we inoculated rat HCC cells via portal vein and allowedthe tumor growth for 3 weeks. For ischemic hypoxia experimentation,the HCC-bearing animals were operated and the hepatic artery wasclamped at designated time intervals of 0, 15, 30 and 45 minutes priorto harvesting the tumor (n = 5 for each time point). Sham controls(without clamping) of the tumor were also harvested at the respec-tive time points. HCC tissues were then subjected to 2-DE PAGEproteomic expression profiling analysis. Among the 1,200 spots iden-tified, about 50 spots demonstrated statistical significance in the timecourse of ischemia as analyzed by one-way ANOVA analysis (P <0.01). By linear regression analysis, we also identified protein speciesthat are associated with hypoxia stress response. Protein spots of interest are being identified using the MALDI-ToF/MS or MS/MSapproach.(supported by grant N_HKU 718/03)
Persistent Identifierhttp://hdl.handle.net/10722/107755
ISSN
2015 Impact Factor: 3.322
2015 SCImago Journal Rankings: 1.190

 

DC FieldValueLanguage
dc.contributor.authorChang, DHHen_HK
dc.contributor.authorYang, Zen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorLuk, JMCen_HK
dc.date.accessioned2010-09-26T00:11:03Z-
dc.date.available2010-09-26T00:11:03Z-
dc.date.issued2006en_HK
dc.identifier.citationShanghai—Hong Kong International Liver Congress, Shanghai, China, 25–28 March 2006. In Journal of Gastroenterology and Hepatology, 2006, v. 21 n. S2, p. A219en_HK
dc.identifier.issn0815-9319en_HK
dc.identifier.urihttp://hdl.handle.net/10722/107755-
dc.description.abstractHCC is the second most prevalent cancer in Hong Kong and China.However, HCC has a high 3-year recurrence rate at 55% after surgery,which limits the long-term survival of HCC patients. One of theimportant variables in any surgical procedure is the organ ischemictime. Several studies had shown that hypoxic stress is an importantdeterminant in both malignant progression and ischemia-reperfusion.Herein, we postulate that hypoxia will result in proteomic expressionchanges in HCC tumors, thereby enhancing tumor recurrence and/orchemoresistance. Using our established orthotopic liver transplanta-tion model, we inoculated rat HCC cells via portal vein and allowedthe tumor growth for 3 weeks. For ischemic hypoxia experimentation,the HCC-bearing animals were operated and the hepatic artery wasclamped at designated time intervals of 0, 15, 30 and 45 minutes priorto harvesting the tumor (n = 5 for each time point). Sham controls(without clamping) of the tumor were also harvested at the respec-tive time points. HCC tissues were then subjected to 2-DE PAGEproteomic expression profiling analysis. Among the 1,200 spots iden-tified, about 50 spots demonstrated statistical significance in the timecourse of ischemia as analyzed by one-way ANOVA analysis (P <0.01). By linear regression analysis, we also identified protein speciesthat are associated with hypoxia stress response. Protein spots of interest are being identified using the MALDI-ToF/MS or MS/MSapproach.(supported by grant N_HKU 718/03)-
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia.en_HK
dc.relation.ispartofJournal of Gastroenterology and Hepatologyen_HK
dc.titleA proteomic study of hypoxia stress response on hepatocellular carcinoma in rat orthotopic liver transplantation modelen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0815-9319&volume=21 Suppl &spage=A219&epage=A219 &date=2006&atitle=A+proteomic+study+of+hypoxia+stress+response+on+hepatocellular+carcinoma+in+rat+orthotopic+liver+transplantation+modelen_HK
dc.identifier.emailChang, DHH: zhang.haoxi@gmail.comen_HK
dc.identifier.emailYang, Z: zfyang@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityLuk, JMC=rp00349en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1746.2006.04408.x-
dc.identifier.hkuros137530en_HK
dc.identifier.volume21en_HK
dc.identifier.spage219en_HK
dc.identifier.epage219en_HK

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