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Conference Paper: Expression analysis of Hoxb5 in enteric neurons and generation of tamoxifen inducible Cre mice for neuronal Hoxb5 signaling perturbation

TitleExpression analysis of Hoxb5 in enteric neurons and generation of tamoxifen inducible Cre mice for neuronal Hoxb5 signaling perturbation
Authors
Issue Date2008
PublisherJapanese Society of Developmental Biologists
Citation
The 41st Annual Meeting for the Japanese Society of Developmental Biologists, Tokushima, Japan, 28-31 May 2008 How to Cite?
AbstractVagal NCC migrate and colonize the intestine rostro-caudally, differentiate into Enteric Nervous System (ENS) ganglia. Expression analysis in human has suggested distinct functions of Hoxb5 at different ENS development stages. Perturbation of Hoxb5 signaling in vagal NCC at the early stage of ENS development impaired NCC migration. However, functions of Hoxb5 in enteric neurons at the late stage of ENS development remains unanswered. In this study, we (i) examine the Hoxb5 expression patterns in ENS in mice; and (ii) generate transgenic mice that express a tamoxifen inducible Cre (MerCreMer) in neurons driven by neuron specific enolase gene (NSE) promoter. In mice, migratory vagal NCC express high level of Hoxb5, and Hoxb5 expression is down-regulated in NCC when these cells undergo differentiation. Later in enteric ganglia, Hoxb5 is re-expressed and the expression persists into adulthood. Co-immunofluorescence analysis revealed Hoxb5 expression in various neuron subtypes, suggesting that Hoxb5 may be involved in the development of different neuron subtypes. Transgenic construct (NSE-MerCreMer) is microinjected to generate founder mice. Four transgenic lines are generated and Cre expressions in neural tissues in these mice are confirmed by RT-PCR, Western blotting, and immuno-fluorescence. Transgenic mice are crossed to reporter lines ROSA26R and Z/AP to evaluate the efficiency and the specificity of tamoxifen inducible cre-recombination. Our NSE-MerCreMer mice shall be an important tool for the investigation of specific roles of Hoxb5 in the development of neurons of ENS.
Persistent Identifierhttp://hdl.handle.net/10722/107437

 

DC FieldValueLanguage
dc.contributor.authorKam, KMen_HK
dc.contributor.authorCheng, WCen_HK
dc.contributor.authorTam, PKHen_HK
dc.contributor.authorLui, VCHen_HK
dc.date.accessioned2010-09-25T23:57:37Z-
dc.date.available2010-09-25T23:57:37Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 41st Annual Meeting for the Japanese Society of Developmental Biologists, Tokushima, Japan, 28-31 May 2008-
dc.identifier.urihttp://hdl.handle.net/10722/107437-
dc.description.abstractVagal NCC migrate and colonize the intestine rostro-caudally, differentiate into Enteric Nervous System (ENS) ganglia. Expression analysis in human has suggested distinct functions of Hoxb5 at different ENS development stages. Perturbation of Hoxb5 signaling in vagal NCC at the early stage of ENS development impaired NCC migration. However, functions of Hoxb5 in enteric neurons at the late stage of ENS development remains unanswered. In this study, we (i) examine the Hoxb5 expression patterns in ENS in mice; and (ii) generate transgenic mice that express a tamoxifen inducible Cre (MerCreMer) in neurons driven by neuron specific enolase gene (NSE) promoter. In mice, migratory vagal NCC express high level of Hoxb5, and Hoxb5 expression is down-regulated in NCC when these cells undergo differentiation. Later in enteric ganglia, Hoxb5 is re-expressed and the expression persists into adulthood. Co-immunofluorescence analysis revealed Hoxb5 expression in various neuron subtypes, suggesting that Hoxb5 may be involved in the development of different neuron subtypes. Transgenic construct (NSE-MerCreMer) is microinjected to generate founder mice. Four transgenic lines are generated and Cre expressions in neural tissues in these mice are confirmed by RT-PCR, Western blotting, and immuno-fluorescence. Transgenic mice are crossed to reporter lines ROSA26R and Z/AP to evaluate the efficiency and the specificity of tamoxifen inducible cre-recombination. Our NSE-MerCreMer mice shall be an important tool for the investigation of specific roles of Hoxb5 in the development of neurons of ENS.-
dc.languageengen_HK
dc.publisherJapanese Society of Developmental Biologists-
dc.relation.ispartofThe Annual Meeting for the Japanese Society of Developmental Biologistsen_HK
dc.titleExpression analysis of Hoxb5 in enteric neurons and generation of tamoxifen inducible Cre mice for neuronal Hoxb5 signaling perturbationen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.emailLui, VCH: vchlui@hkucc.hku.hken_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.identifier.authorityLui, VCH=rp00363en_HK
dc.identifier.hkuros142734en_HK

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