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Conference Paper: Increased expression of SPARC and SPARC-like 1 in relation to tumor angiogenesis in hepatocellular carcinoma

TitleIncreased expression of SPARC and SPARC-like 1 in relation to tumor angiogenesis in hepatocellular carcinoma
Authors
Issue Date2004
PublisherAmerican Association for Cancer Research
Citation
AACR 95th Annual Meeting, Orlando FL, 27–31 March 2004. In Cancer Research, 2004, v. 64 n. 7S, p. 754 Abstract no. 3256 How to Cite?
AbstractBackground: Tumor angiogenesis, the growth of new blood vessels in tumor, is fundamental to tumor growth and metastasis of tumor cells. Our research group has identified two candidate genes for tumor angiogenesis, SPARC (Secreted Protein Acidic and Rich in Cysteine) and SPARC-like 1, in HCC through gene expression study using cDNA microarray technology. Objective: The aim of the present study was to quantify the expression of SPARC and SPARC-like 1 in HCC and to investigate the relationship between the expression of these genes and the increase in tumor angiogenesis in HCC. Methods: SPARC and SPARC-like 1 mRNA expression in HCC and nontumorous liver of 55 patients was quantitated by real-time RT-PCR. SPARC protein expression was assessed by immunohistochemical staining. The data were then correlated with the result of immunostaining for CD34, which is an endothelial cell marker for HCC angiogenesis. Result: Tumor SPARC mRNA level correlated significantly with tumor SPARC-like 1 mRNA level (p<0.001). Similarly, non-tumor SPARC mRNA level correlated significantly with non-tumor SPARC-like 1 mRNA level (p<0.001). This finding suggested that SPARC and SPARC-like 1 might be co-expressed. Further analysis using paired sample t test indicated that there was a significant difference between tumor and non-tumor SPARC mRNA level (mean 18.54 vs. 4.82, p<0.001). Likewise, there was a significant difference between tumor and non-tumor SPARC-like 1 mRNA level (mean 12.94 vs. 2.87, p<0.001). Immunohistochemical staining data indicated that tumor parenchymal SPARC protein expression was significantly correlated with tumor CD34 immunostaining (p<0.01). Similarly, tumor SPARC-like 1 mRNA expression was also significantly correlated with tumor CD34 immunostaining (p<0.05). Conclusions: Our results indicate that SPARC and SPARC-like 1 are overexpressed in HCC compared with nontumorous liver, and both genes may play a role in HCC angiogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/106888
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372

 

DC FieldValueLanguage
dc.contributor.authorLau, CPYen_HK
dc.contributor.authorPoon, RTPen_HK
dc.contributor.authorCheung, STen_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2010-09-25T23:34:42Z-
dc.date.available2010-09-25T23:34:42Z-
dc.date.issued2004en_HK
dc.identifier.citationAACR 95th Annual Meeting, Orlando FL, 27–31 March 2004. In Cancer Research, 2004, v. 64 n. 7S, p. 754 Abstract no. 3256-
dc.identifier.issn0008-5472-
dc.identifier.urihttp://hdl.handle.net/10722/106888-
dc.description.abstractBackground: Tumor angiogenesis, the growth of new blood vessels in tumor, is fundamental to tumor growth and metastasis of tumor cells. Our research group has identified two candidate genes for tumor angiogenesis, SPARC (Secreted Protein Acidic and Rich in Cysteine) and SPARC-like 1, in HCC through gene expression study using cDNA microarray technology. Objective: The aim of the present study was to quantify the expression of SPARC and SPARC-like 1 in HCC and to investigate the relationship between the expression of these genes and the increase in tumor angiogenesis in HCC. Methods: SPARC and SPARC-like 1 mRNA expression in HCC and nontumorous liver of 55 patients was quantitated by real-time RT-PCR. SPARC protein expression was assessed by immunohistochemical staining. The data were then correlated with the result of immunostaining for CD34, which is an endothelial cell marker for HCC angiogenesis. Result: Tumor SPARC mRNA level correlated significantly with tumor SPARC-like 1 mRNA level (p<0.001). Similarly, non-tumor SPARC mRNA level correlated significantly with non-tumor SPARC-like 1 mRNA level (p<0.001). This finding suggested that SPARC and SPARC-like 1 might be co-expressed. Further analysis using paired sample t test indicated that there was a significant difference between tumor and non-tumor SPARC mRNA level (mean 18.54 vs. 4.82, p<0.001). Likewise, there was a significant difference between tumor and non-tumor SPARC-like 1 mRNA level (mean 12.94 vs. 2.87, p<0.001). Immunohistochemical staining data indicated that tumor parenchymal SPARC protein expression was significantly correlated with tumor CD34 immunostaining (p<0.01). Similarly, tumor SPARC-like 1 mRNA expression was also significantly correlated with tumor CD34 immunostaining (p<0.05). Conclusions: Our results indicate that SPARC and SPARC-like 1 are overexpressed in HCC compared with nontumorous liver, and both genes may play a role in HCC angiogenesis.-
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research-
dc.relation.ispartofCancer Researchen_HK
dc.titleIncreased expression of SPARC and SPARC-like 1 in relation to tumor angiogenesis in hepatocellular carcinomaen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLau, CPY: cpylau@hkucc.hku.hken_HK
dc.identifier.emailPoon, RTP: poontp@hkucc.hku.hken_HK
dc.identifier.emailCheung, ST: stcheung@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityPoon, RTP=rp00446en_HK
dc.identifier.authorityCheung, ST=rp00457en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.hkuros92369en_HK

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