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Conference Paper: Contractions of the SHR aorta to high doses of epigallocatechin gallate are due to vasoconstrictor prostanoids

TitleContractions of the SHR aorta to high doses of epigallocatechin gallate are due to vasoconstrictor prostanoids
Authors
KeywordsBiology
Issue Date2010
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
The Experimental Biology 2010, Anaheim, CA., 24-28 April 2010. In The FASEB Journal, 2010, v. 24 Meeting abstract suppl., abstract no. 960.2 How to Cite?
AbstractOBJECTIVE: Regular consumption of green tea is believed to be beneficial for the cardiovascular system. However, high concentrations of epigallocatechin gallate (EGCG), a catechin of green tea, cause contractions of the aorta of spontaneously hypertensive rats (SHR). The present studies were designed to investigate the mechanisms underlying these EGCG-induced contractions. METHODS: Isometric tension was measured in isolated aortic rings from 36-weeks-old male SHR. The release of prostanoids end-products was measured with enzyme immunoassay kits. The intracellular reactive oxygen species (ROS) concentration was measured by confocal microscopy. RESULTS: From 10(–6) to 10(–4) M, EGCG induced concentration-dependent contractions in preparations with and without endothelium. These contractions were abolished by cyclooxygenase (COX) inhibitor indomethacin or the thromboxane-prostanoid (TP) receptor antagonist S18886, and reduced by antioxidants. The release of prostaglandin F1α, prostaglandin F2α and thromboxane B2 was significantly increased by 10(–4) M EGCG. A burst of ROS was observed upon exposure to 10(–4) M of EGCG. CONCLUSIONS: The EGCG-induced contractions involve the production of ROS, activation of COX, production of prostanoids and stimulation of TP receptors. This study indicates a possible adverse effect of EGCG administration and tea consumption at high concentrations.
Persistent Identifierhttp://hdl.handle.net/10722/106839
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorLi, Zen_HK
dc.contributor.authorKoo, MWLen_HK
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.date.accessioned2010-09-25T23:32:34Z-
dc.date.available2010-09-25T23:32:34Z-
dc.date.issued2010en_HK
dc.identifier.citationThe Experimental Biology 2010, Anaheim, CA., 24-28 April 2010. In The FASEB Journal, 2010, v. 24 Meeting abstract suppl., abstract no. 960.2en_HK
dc.identifier.issn0892-6638-
dc.identifier.urihttp://hdl.handle.net/10722/106839-
dc.description.abstractOBJECTIVE: Regular consumption of green tea is believed to be beneficial for the cardiovascular system. However, high concentrations of epigallocatechin gallate (EGCG), a catechin of green tea, cause contractions of the aorta of spontaneously hypertensive rats (SHR). The present studies were designed to investigate the mechanisms underlying these EGCG-induced contractions. METHODS: Isometric tension was measured in isolated aortic rings from 36-weeks-old male SHR. The release of prostanoids end-products was measured with enzyme immunoassay kits. The intracellular reactive oxygen species (ROS) concentration was measured by confocal microscopy. RESULTS: From 10(–6) to 10(–4) M, EGCG induced concentration-dependent contractions in preparations with and without endothelium. These contractions were abolished by cyclooxygenase (COX) inhibitor indomethacin or the thromboxane-prostanoid (TP) receptor antagonist S18886, and reduced by antioxidants. The release of prostaglandin F1α, prostaglandin F2α and thromboxane B2 was significantly increased by 10(–4) M EGCG. A burst of ROS was observed upon exposure to 10(–4) M of EGCG. CONCLUSIONS: The EGCG-induced contractions involve the production of ROS, activation of COX, production of prostanoids and stimulation of TP receptors. This study indicates a possible adverse effect of EGCG administration and tea consumption at high concentrations.-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/-
dc.relation.ispartofThe FASEB Journalen_HK
dc.subjectBiology-
dc.titleContractions of the SHR aorta to high doses of epigallocatechin gallate are due to vasoconstrictor prostanoidsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0892-6638&volume=24, Meeting abstract suppl., abstract no. 960.2&spage=&epage=&date=2010&atitle=Contractions+of+the+SHR+aorta+to+high+doses+of+epigallocatechin+gallate+are+due+to+vasoconstrictor+prostanoids-
dc.identifier.emailLi, Z: winnieli@ust.hken_HK
dc.identifier.emailKoo, MWL: wlkoo@hkusua.hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.hkuros170024en_HK
dc.identifier.volume24en_HK
dc.identifier.issueMeeting abstract suppl.-
dc.description.otherThe Experimental Biology 2010, Anaheim, CA., 24-28 April 2010. In The FASEB Journal, 2010, v. 24 Meeting abstract suppl., abstract no. 960.2-

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