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Conference Paper: Suppression of nitric oxide generation by antioxidants in alveolar macrophage-derived cell line MH-S

TitleSuppression of nitric oxide generation by antioxidants in alveolar macrophage-derived cell line MH-S
Authors
Issue Date1998
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomed
Citation
The 5th Annual Meeting of the Oxygen Society, Washington, DC., 19–23 November 1998. In Free Radical Biology & Medicine, 1998, v. 25 suppl. 1, p. S40, abstract no. 96 How to Cite?
AbstractPreincubation of alveolar macrophages (AM) with bacterial endotoxin &I’S) enhances macrophage resistance to intracellular replication of pathogens and potentiates the activating effect of interferon-gamma (IFN-gamma). Such immunologic activation also induces the expression of nitric oxide synthase (iNOS) to generate reactive nitric oxide (NO) which contributes to the antimicrobial activity against pathogens. With the use of MH-S cell line derived from murine AM, we have examined the stimulatory effects of LPS (0.001 mg/ml) and IFN-gamma (100 U/ml) on extracellular NO production. The NO level in the culture medium was l&fold higher than basal value in 18 hours. In the p-ce of N(G)-methyl-L-arginine (L-NMMA, 1 mM), the amount of NO generated was suppressed to 19.3%, indicating that the in- in NO was the consequence of expression of iNOS. S-Methylisothiourea (SMT), a highly selective inhibitor of iNOS, was found to be very effective with an IC, of 0.01 mM concentration. Pyrrolidine dithiocarbamate (PDTC) and diethyldithiocarbamate (DETC) were both strong inhibitors of inducible NO production with IC, of 0.04 and 0.05 mM, respectively. Other antioxidank, e.g. N-acetylcysteine, also exhibit inhibitory effects. but comparatively weaker (IC, = 4 mM). To investigate the role of poly-ADP-ribosylation in iNO! induction, poly_(ADP-ribose) poly-merase (PARP) inhibitors, e.g. 3_aminobenzamide, were examined for their effects on NO production. It was found that all PARP inhibitors are weak inhibitors with IC,, in the range of 10-25 mM. In conclusion, the established transformed murine AM MH-S cell line can be useful in the study of cytokines and oxidative stress in microbicidal defenses of alveolar macrophages.
Persistent Identifierhttp://hdl.handle.net/10722/106815
ISSN
2015 Impact Factor: 5.784
2015 SCImago Journal Rankings: 2.468

 

DC FieldValueLanguage
dc.contributor.authorTom, WMen_HK
dc.contributor.authorWong, DWKen_HK
dc.contributor.authorWong, EYKen_HK
dc.date.accessioned2010-09-25T23:31:34Z-
dc.date.available2010-09-25T23:31:34Z-
dc.date.issued1998en_HK
dc.identifier.citationThe 5th Annual Meeting of the Oxygen Society, Washington, DC., 19–23 November 1998. In Free Radical Biology & Medicine, 1998, v. 25 suppl. 1, p. S40, abstract no. 96en_HK
dc.identifier.issn0891-5849en_HK
dc.identifier.urihttp://hdl.handle.net/10722/106815-
dc.description.abstractPreincubation of alveolar macrophages (AM) with bacterial endotoxin &I’S) enhances macrophage resistance to intracellular replication of pathogens and potentiates the activating effect of interferon-gamma (IFN-gamma). Such immunologic activation also induces the expression of nitric oxide synthase (iNOS) to generate reactive nitric oxide (NO) which contributes to the antimicrobial activity against pathogens. With the use of MH-S cell line derived from murine AM, we have examined the stimulatory effects of LPS (0.001 mg/ml) and IFN-gamma (100 U/ml) on extracellular NO production. The NO level in the culture medium was l&fold higher than basal value in 18 hours. In the p-ce of N(G)-methyl-L-arginine (L-NMMA, 1 mM), the amount of NO generated was suppressed to 19.3%, indicating that the in- in NO was the consequence of expression of iNOS. S-Methylisothiourea (SMT), a highly selective inhibitor of iNOS, was found to be very effective with an IC, of 0.01 mM concentration. Pyrrolidine dithiocarbamate (PDTC) and diethyldithiocarbamate (DETC) were both strong inhibitors of inducible NO production with IC, of 0.04 and 0.05 mM, respectively. Other antioxidank, e.g. N-acetylcysteine, also exhibit inhibitory effects. but comparatively weaker (IC, = 4 mM). To investigate the role of poly-ADP-ribosylation in iNO! induction, poly_(ADP-ribose) poly-merase (PARP) inhibitors, e.g. 3_aminobenzamide, were examined for their effects on NO production. It was found that all PARP inhibitors are weak inhibitors with IC,, in the range of 10-25 mM. In conclusion, the established transformed murine AM MH-S cell line can be useful in the study of cytokines and oxidative stress in microbicidal defenses of alveolar macrophages.-
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/freeradbiomeden_HK
dc.relation.ispartofFree Radical Biology & Medicineen_HK
dc.rightsFree Radical Biology & Medicine. Copyright © Elsevier Inc.en_HK
dc.titleSuppression of nitric oxide generation by antioxidants in alveolar macrophage-derived cell line MH-Sen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0891-5849&volume=25&spage=S40&epage=&date=1998&atitle=Suppression+of+nitric+oxide+generation+by+antioxidants+in+alveolar+macrophage-derived+cell+line+MH-Sen_HK
dc.identifier.emailTom, WM: wmtoma@hkucc.hku.hken_HK
dc.identifier.emailWong, EYK: eykwong@HKUCC.hku.hken_HK
dc.identifier.authorityTom, WM=rp00237en_HK
dc.identifier.doi10.1016/S0891-5849(98)90121-6-
dc.identifier.hkuros45679en_HK
dc.identifier.volume25en_HK
dc.identifier.issuesuppl. 1-
dc.identifier.spageS40, abstract no. 96en_HK
dc.identifier.epageS40, abstract no. 96-

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