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Conference Paper: Nitric oxide synthase and soluble guanylyl cyclase activation are required for hypoxic endothelium-dependent contractions of the porcine coronary artery

TitleNitric oxide synthase and soluble guanylyl cyclase activation are required for hypoxic endothelium-dependent contractions of the porcine coronary artery
Authors
KeywordsBiology
Issue Date2010
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
The Experimental Biology 2010, Anaheim, CA., 24-28 April 2010. In The FASEB Journal, 2010, v. 24 Meeting abstract suppl., abstract no. 957.2 How to Cite?
AbstractThe vasoconstrictor response to hypoxia in the lung, termed hypoxic pulmonary vasoconstriction, is also observed in coronary arteries. The present study examined the mechanism underlying hypoxic contractions in isolated porcine coronary arteries. Isometric tension was measured in rings with or without endothelium. In quiescent preparations, the contractile response to hypoxia was only observed in rings with endothelium and was abolished by indomethacin and S18886, demonstrating the involvement of cyclooxygenase products and TP receptor activation, respectively. In contracted preparations, the hypoxic response was also endothelium-dependent and was reduced by indomethacin and S18886. The endothelium-dependent hypoxic contractions were abolished by L-NAME, ODQ and NS2028 in both quiescent and contracted preparations. The addition of DetaNONOate in the presence of L-NAME restored the hypoxic response, suggesting the involvement of the nitric oxide pathway. Assay of the cyclic GMP content showed no change upon exposure to hypoxia in preparations with and without endothelium. The relaxation to 8-Br-cGMP was not reduced during hypoxia, meaning that the hypoxic contraction is not due to abolition of a basal relaxing component. These experiments suggest that hypoxic endothelium-dependent contractions of the porcine coronary artery depend on more than one signaling pathway.
Persistent Identifierhttp://hdl.handle.net/10722/106789
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorChan, CKYen_HK
dc.contributor.authorMak, JCWen_HK
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.date.accessioned2010-09-25T23:30:29Z-
dc.date.available2010-09-25T23:30:29Z-
dc.date.issued2010en_HK
dc.identifier.citationThe Experimental Biology 2010, Anaheim, CA., 24-28 April 2010. In The FASEB Journal, 2010, v. 24 Meeting abstract suppl., abstract no. 957.2en_HK
dc.identifier.issn0892-6638-
dc.identifier.urihttp://hdl.handle.net/10722/106789-
dc.description.abstractThe vasoconstrictor response to hypoxia in the lung, termed hypoxic pulmonary vasoconstriction, is also observed in coronary arteries. The present study examined the mechanism underlying hypoxic contractions in isolated porcine coronary arteries. Isometric tension was measured in rings with or without endothelium. In quiescent preparations, the contractile response to hypoxia was only observed in rings with endothelium and was abolished by indomethacin and S18886, demonstrating the involvement of cyclooxygenase products and TP receptor activation, respectively. In contracted preparations, the hypoxic response was also endothelium-dependent and was reduced by indomethacin and S18886. The endothelium-dependent hypoxic contractions were abolished by L-NAME, ODQ and NS2028 in both quiescent and contracted preparations. The addition of DetaNONOate in the presence of L-NAME restored the hypoxic response, suggesting the involvement of the nitric oxide pathway. Assay of the cyclic GMP content showed no change upon exposure to hypoxia in preparations with and without endothelium. The relaxation to 8-Br-cGMP was not reduced during hypoxia, meaning that the hypoxic contraction is not due to abolition of a basal relaxing component. These experiments suggest that hypoxic endothelium-dependent contractions of the porcine coronary artery depend on more than one signaling pathway.-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/-
dc.relation.ispartofThe FASEB Journalen_HK
dc.subjectBiology-
dc.titleNitric oxide synthase and soluble guanylyl cyclase activation are required for hypoxic endothelium-dependent contractions of the porcine coronary arteryen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0892-6638&volume=24, Meeting abstract suppl., abstract no. 957.2&spage=&epage=&date=2010&atitle=Nitric+oxide+synthase+and+soluble+guanylyl+cyclase+activation+are+required+for+hypoxic+endothelium-dependent+contractions+of+the+porcine+coronary+artery-
dc.identifier.emailChan, CKY: chancalvink@gmail.comen_HK
dc.identifier.emailMak, JCW: judymak@HKUCC.hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.hkuros170025en_HK
dc.identifier.volume24en_HK
dc.identifier.issueMeeting abstract suppl.-
dc.description.otherThe Experimental Biology 2010, Anaheim, CA., 24-28 April 2010. In The FASEB Journal, 2010, v. 24 Meeting abstract suppl., abstract no. 957.2-

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