File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL

Conference Paper: Real-time quantitative PCR study of genes involved in the generation of endothelium-depedent contractions in the aorta of the spontaneously hypertensive rat

TitleReal-time quantitative PCR study of genes involved in the generation of endothelium-depedent contractions in the aorta of the spontaneously hypertensive rat
Authors
Issue Date2006
PublisherFederation of American Societies for Experimental Biology.
Citation
The F A S E B Journal, 2006, v. 20 n. 4, p. A290-A291 How to Cite?
AbstractActivation of muscarinic receptors by acetylcholine causes larger endothelium-dependent contractions in arteries of spontaneously hypertensive (SHR) than normotensive (WKY) rats. Such endothelium-dependent contractions involve the release of endoperoxides and prostacyclin which activate thromboxane-prostanoid (TP) receptors on the vascular smooth muscle (VSM). The present study aimed to define whether or not the expression of the different muscarinic receptor subtypes (m1-m5) differs in aortic endothelial cells (EC) of the SHR. The mRNA expression of cyclooxygenase-1 (COX-1), prostacyclin synthase and thromboxane synthase, and of prostacyclin and TP-receptors was measured, using real-time PCR, in freshly isolated EC and VSM. The expression of the five muscarinic receptor subtypes in EC was comparable in the two strains. The abundance of the individual muscarinic subtypes in descending order was: m3>m2>m1, with little to no m4 or m5 expressed. The mRNA expression of COX-1 and of prostacyclin and thromboxane synthases was elevated in EC of SHR. The gene expression of prostacyclin or TP-receptors was comparable in VSM of the two strains. These data suggest that an alteration in muscarinic receptors on EC is unlikely to explain the enhanced endothelium-dependent contractions in the SHR. However, an elevated expression of COX-1 and of prostacyclin and thromboxane synthases probably contribute to the greater production of endothelium-derived prostanoids observed in arteries of the hypertensive rats.
Persistent Identifierhttp://hdl.handle.net/10722/106776
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorTang, HCen_HK
dc.contributor.authorLeung, GPHen_HK
dc.contributor.authorLo, ACYen_HK
dc.contributor.authorChung, SKen_HK
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorVanhoutte, PMGRen_HK
dc.date.accessioned2010-09-25T23:29:57Z-
dc.date.available2010-09-25T23:29:57Z-
dc.date.issued2006en_HK
dc.identifier.citationThe F A S E B Journal, 2006, v. 20 n. 4, p. A290-A291en_HK
dc.identifier.issn0892-6638en_HK
dc.identifier.urihttp://hdl.handle.net/10722/106776-
dc.description.abstractActivation of muscarinic receptors by acetylcholine causes larger endothelium-dependent contractions in arteries of spontaneously hypertensive (SHR) than normotensive (WKY) rats. Such endothelium-dependent contractions involve the release of endoperoxides and prostacyclin which activate thromboxane-prostanoid (TP) receptors on the vascular smooth muscle (VSM). The present study aimed to define whether or not the expression of the different muscarinic receptor subtypes (m1-m5) differs in aortic endothelial cells (EC) of the SHR. The mRNA expression of cyclooxygenase-1 (COX-1), prostacyclin synthase and thromboxane synthase, and of prostacyclin and TP-receptors was measured, using real-time PCR, in freshly isolated EC and VSM. The expression of the five muscarinic receptor subtypes in EC was comparable in the two strains. The abundance of the individual muscarinic subtypes in descending order was: m3>m2>m1, with little to no m4 or m5 expressed. The mRNA expression of COX-1 and of prostacyclin and thromboxane synthases was elevated in EC of SHR. The gene expression of prostacyclin or TP-receptors was comparable in VSM of the two strains. These data suggest that an alteration in muscarinic receptors on EC is unlikely to explain the enhanced endothelium-dependent contractions in the SHR. However, an elevated expression of COX-1 and of prostacyclin and thromboxane synthases probably contribute to the greater production of endothelium-derived prostanoids observed in arteries of the hypertensive rats.-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology.en_HK
dc.relation.ispartofThe F A S E B Journalen_HK
dc.titleReal-time quantitative PCR study of genes involved in the generation of endothelium-depedent contractions in the aorta of the spontaneously hypertensive raten_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0892-6638&volume=20&issue=4&spage=A290&epage=A291 Part 1&date=2006&atitle=Real-time+quantitative+PCR+study+of+genes+involved+in+the+generation+of+endothelium-depedent+contractions+in+the+aorta+of+the+spontaneously+hypertensive+raten_HK
dc.identifier.emailLeung, GPH: leung_pak_heng@hotmail.comen_HK
dc.identifier.emailLo, ACY: amylo@hkucc.hku.hken_HK
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.emailVanhoutte, PMGR: vanhoutt@hku.hken_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.identifier.authorityLo, ACY=rp00425en_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.identifier.hkuros122046en_HK
dc.identifier.volume20en_HK
dc.identifier.issue4en_HK
dc.identifier.spage290en_HK
dc.identifier.epage291en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats