File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: Rapid, non-genomic vascular actions of genistein suggests a phytoestrogen receptor

TitleRapid, non-genomic vascular actions of genistein suggests a phytoestrogen receptor
Authors
Issue Date2008
PublisherFederation of American Societies for Experimental Biology. Meeting abstracts can be accessed via http://www.fasebj.org/search.dtl
Citation
Experimental Biology 2008 - ASPET's Centennial Meeting, San Diego, CA, 5-9 April 2008. In The FASEB Journal, 2008, v. 22 n. Meeting Abstract Supplement, p. 912.14 How to Cite?
AbstractGenistein, a phytoestrogen rich in soy beans and soy products, was reported to be a vasorelaxant. This study examined the receptor and related signaling pathways in the rapid vascular actions of genistein. Isometric tension was measured in isolated aortic rings from male spontaneous hypertensive rats (SHR). Acute exposure to genistein at 10 µM, a concentration with no direct relaxation effect, potentiated acetylcholine (ACh)-induced relaxation and reduced ACh-induced contraction. Both actions were insensitive to 10 µM actionmycin D (transcription inhibitor) and 10 µM cycloheximide (translation inhibitor). The potentiation of ACh-induced relaxation by genistein was inhibited by 10 µM NF023 and 10 µM GP antagonist-2A, the selective Gi/o and Gq{alpha}-subunit antagonists respectively, but not by 10 µM NF449, a selective Gs{alpha}-subunit antagonist. Interestingly, NF023, NF449 and GP antagonist-2A did not alter the inhibitory effect of genistein on ACh-induced contraction. These results demonstrate that rapid vascular actions of genistein in modulating ACh-induced relaxation and contraction responses in SHR are mediated by non-genomic pathways. Furthermore, involvement of G-proteins in the enhancement of ACh-induced relaxation by genistein suggests that genistein exerts its effect through a putative G-protein coupled phytoestrogen receptor. (Supported by the Research Grant Council of Hong Kong)
Persistent Identifierhttp://hdl.handle.net/10722/106749
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorLin, HYAen_HK
dc.contributor.authorLeung, GPHen_HK
dc.contributor.authorLeung, SWSen_HK
dc.contributor.authorMan, RYKen_HK
dc.date.accessioned2010-09-25T23:28:50Z-
dc.date.available2010-09-25T23:28:50Z-
dc.date.issued2008en_HK
dc.identifier.citationExperimental Biology 2008 - ASPET's Centennial Meeting, San Diego, CA, 5-9 April 2008. In The FASEB Journal, 2008, v. 22 n. Meeting Abstract Supplement, p. 912.14en_HK
dc.identifier.issn0892-6638-
dc.identifier.urihttp://hdl.handle.net/10722/106749-
dc.description.abstractGenistein, a phytoestrogen rich in soy beans and soy products, was reported to be a vasorelaxant. This study examined the receptor and related signaling pathways in the rapid vascular actions of genistein. Isometric tension was measured in isolated aortic rings from male spontaneous hypertensive rats (SHR). Acute exposure to genistein at 10 µM, a concentration with no direct relaxation effect, potentiated acetylcholine (ACh)-induced relaxation and reduced ACh-induced contraction. Both actions were insensitive to 10 µM actionmycin D (transcription inhibitor) and 10 µM cycloheximide (translation inhibitor). The potentiation of ACh-induced relaxation by genistein was inhibited by 10 µM NF023 and 10 µM GP antagonist-2A, the selective Gi/o and Gq{alpha}-subunit antagonists respectively, but not by 10 µM NF449, a selective Gs{alpha}-subunit antagonist. Interestingly, NF023, NF449 and GP antagonist-2A did not alter the inhibitory effect of genistein on ACh-induced contraction. These results demonstrate that rapid vascular actions of genistein in modulating ACh-induced relaxation and contraction responses in SHR are mediated by non-genomic pathways. Furthermore, involvement of G-proteins in the enhancement of ACh-induced relaxation by genistein suggests that genistein exerts its effect through a putative G-protein coupled phytoestrogen receptor. (Supported by the Research Grant Council of Hong Kong)-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology. Meeting abstracts can be accessed via http://www.fasebj.org/search.dtl-
dc.relation.ispartofThe FASEB Journalen_HK
dc.titleRapid, non-genomic vascular actions of genistein suggests a phytoestrogen receptoren_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLeung, GPH: leung_pak_heng@hotmail.comen_HK
dc.identifier.emailLeung, SWS: swsleung@HKUCC.hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.identifier.authorityLeung, SWS=rp00235en_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.identifier.hkuros152555en_HK
dc.identifier.spage173en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats