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Conference Paper: Effects of epoxyeicosatrienoic acids on volume-activated chloride channels via cyclic GMP pathway in rat mesenteric artery

TitleEffects of epoxyeicosatrienoic acids on volume-activated chloride channels via cyclic GMP pathway in rat mesenteric artery
Authors
Issue Date2008
PublisherFederation of American Societies for Experimental Biology. Meeting abstracts can be accessed via http://www.fasebj.org/search.dtl
Citation
The 2008 Annual Meeting of Experimental Biology (EB 2008) - ASPET's Centennial Meeting, San Diego, CA., 5-9 April 2008. In The FASEB Journal, 2008, v. 22 meeting abstract suppl., abstract no. 912.30 How to Cite?
AbstractEpoxyeicosatrienoic acids (EETs) are one of the proposed candidates of EDHF. It is known that volume-activated chloride channels (VACCs) play important roles in regulating cell membrane potential, so we hypothesize that VACCs may be involved in EETs-induced vasorelaxation. Rat mesenteric arteries were dissected out and the arteries rings were relaxed by acetylcholine (ACh, 1 nM to 1 mM) in the presence of L-NAME and indomethacin. It was of interest to note that the ACh (1 µM)-induced relaxation was reduced by about 60% when the arteries were precontracted by 60 mM KCl instead of phenylephrine (1 µM). In addition, the ACh (1 µM)-induced relaxations were almost abolished by EETs antagonist (14, 15 epoxyeicosa-5(Z)-enoic acid, 1 µM). Whole cell patch clamping demonstrated that VACC currents in primary cultured rat mesenteric arterial smooth muscle cells were partly inhibited by EETs. However, the inhibition effects were abolished by cyclic GMP (cGMP) antagonists (Rp-cGMP, 100 µM), and cGMP-dependent protein kinase inhibitors (KT-5823, 0.3 µM). Our results suggest that EETs-induced relaxations of mesenteric arteries were partly mediated by inhibition of VACCs through cGMP pathway. (Supported by the Research Grant Council of Hong Kong)
Persistent Identifierhttp://hdl.handle.net/10722/106693
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorYang, Cen_HK
dc.contributor.authorVanhoutte, PMGRen_HK
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorLeung, GPHen_HK
dc.date.accessioned2010-09-25T23:26:31Z-
dc.date.available2010-09-25T23:26:31Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 2008 Annual Meeting of Experimental Biology (EB 2008) - ASPET's Centennial Meeting, San Diego, CA., 5-9 April 2008. In The FASEB Journal, 2008, v. 22 meeting abstract suppl., abstract no. 912.30-
dc.identifier.issn0892-6638-
dc.identifier.urihttp://hdl.handle.net/10722/106693-
dc.description.abstractEpoxyeicosatrienoic acids (EETs) are one of the proposed candidates of EDHF. It is known that volume-activated chloride channels (VACCs) play important roles in regulating cell membrane potential, so we hypothesize that VACCs may be involved in EETs-induced vasorelaxation. Rat mesenteric arteries were dissected out and the arteries rings were relaxed by acetylcholine (ACh, 1 nM to 1 mM) in the presence of L-NAME and indomethacin. It was of interest to note that the ACh (1 µM)-induced relaxation was reduced by about 60% when the arteries were precontracted by 60 mM KCl instead of phenylephrine (1 µM). In addition, the ACh (1 µM)-induced relaxations were almost abolished by EETs antagonist (14, 15 epoxyeicosa-5(Z)-enoic acid, 1 µM). Whole cell patch clamping demonstrated that VACC currents in primary cultured rat mesenteric arterial smooth muscle cells were partly inhibited by EETs. However, the inhibition effects were abolished by cyclic GMP (cGMP) antagonists (Rp-cGMP, 100 µM), and cGMP-dependent protein kinase inhibitors (KT-5823, 0.3 µM). Our results suggest that EETs-induced relaxations of mesenteric arteries were partly mediated by inhibition of VACCs through cGMP pathway. (Supported by the Research Grant Council of Hong Kong)-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology. Meeting abstracts can be accessed via http://www.fasebj.org/search.dtlen_HK
dc.relation.ispartofThe FASEB Journalen_HK
dc.titleEffects of epoxyeicosatrienoic acids on volume-activated chloride channels via cyclic GMP pathway in rat mesenteric arteryen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0176-8638&volume=&spage=&epage=&date=2008&atitle=Effects+of+epoxyeicosatrienoic+acids+on+volume-activated+chloride+channels+via+cyclic+GMP+pathway+in+rat+mesenteric+arteryen_HK
dc.identifier.emailYang, C: yangnjmu@yahoo.com.cnen_HK
dc.identifier.emailVanhoutte, PMGR: vanhoutt@hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.emailLeung, GPH: gphleung@hku.hken_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.identifier.hkuros143136en_HK
dc.identifier.volume22-
dc.identifier.issuemeeting abstract suppl., abstract no. 912.30-

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