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Conference Paper: Bioactivity-guided identification and cell signaling analysis to investigate the immunomodulatory effects of ginseng on U937 cells
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TitleBioactivity-guided identification and cell signaling analysis to investigate the immunomodulatory effects of ginseng on U937 cells
 
AuthorsLee, DCW
Yang, LH
Chik, SCC
Li, CB
Lau, ASY
 
Issue Date2009
 
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/cytokine
 
CitationTri-Society Annual Conference 2009 of the Society for Leukocyte Biology, International Cytokine Society & International Society for Interferon and Cytokine Research, Cellular and Cytokine Interactions in Health and Disease, Lisbon, Portugal, 17-21 October, 2009 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.cyto.2009.07.424
 
AbstractPanax ginseng (Ginseng) is one of the most commonly used medicinal herbs worldwide. It is believed to have beneficial effects against human diseases, and its active components, ginsenosides, may play critical roles in its diverse physiological actions. However, the mechanisms underlying ginseng’s effects remain to be investigated. We hypothesize that some biological effects of ginseng are due to its anti-inflammatory activities. To investigate the effects of ginseng on inflammation, human monocytic cells (U937) were treated sequentially with 70% ethanol–water extracts of Panax ginseng (PGSE) and tumor necrosis factor-alpha (TNF-α). Gene expression profiles of the cells were examined by genechip analysis (Human Genome U133 Plus 2.0 arrays, Affymetrix) and validated by Taqman quantitative RT-PCR, and protein expressions were assayed by ELISA. The composition of ginsenosides in 70% ethanol–water extracts of ginseng was analyzed by HPLC. Activation of signaling kinases was examined by Western blot analysis and the respective anti-phospho-protein kinase antibodies. PGSE significantly inhibited the transcription and secretion of CXCL-10 following TNF-α stimulation. Nine ginsenosides including Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg3 and Rh1 were identified in the PGSE by HPLC. Seven out of nine ginsenosides could significantly inhibit TNF-α-induced CXCL-10 expression in U937 cells but the CXCL-10 suppressive effects of individual ginsenosides was less than that of the crude extract or the mixture of reconstituted ginsenosides. The suppressive effect of the reconstituted mixture of nine ginsenosides at the corresponding doses was comparable to the PGSE treatment in a dose-dependent manner. Furthermore, the CXCL-10 suppression can be correlated with the inactivation of ERK1/2 pathways by the PGSE. In summary, our results provide evidence that ginseng can suppress TNF-α-inducible cytokines and signaling proteins in promonocytic cells.
 
DescriptionCytokine, 2009, v. 48 n. 1-2, p. 101 abstract no. PP2-046
Poster Presentation II
 
ISSN1043-4666
2012 Impact Factor: 2.518
2012 SCImago Journal Rankings: 0.968
 
DOIhttp://dx.doi.org/10.1016/j.cyto.2009.07.424
 
ISI Accession Number IDWOS:000270855100355
 
DC FieldValue
dc.contributor.authorLee, DCW
 
dc.contributor.authorYang, LH
 
dc.contributor.authorChik, SCC
 
dc.contributor.authorLi, CB
 
dc.contributor.authorLau, ASY
 
dc.date.accessioned2010-09-25T22:46:30Z
 
dc.date.available2010-09-25T22:46:30Z
 
dc.date.issued2009
 
dc.description.abstractPanax ginseng (Ginseng) is one of the most commonly used medicinal herbs worldwide. It is believed to have beneficial effects against human diseases, and its active components, ginsenosides, may play critical roles in its diverse physiological actions. However, the mechanisms underlying ginseng’s effects remain to be investigated. We hypothesize that some biological effects of ginseng are due to its anti-inflammatory activities. To investigate the effects of ginseng on inflammation, human monocytic cells (U937) were treated sequentially with 70% ethanol–water extracts of Panax ginseng (PGSE) and tumor necrosis factor-alpha (TNF-α). Gene expression profiles of the cells were examined by genechip analysis (Human Genome U133 Plus 2.0 arrays, Affymetrix) and validated by Taqman quantitative RT-PCR, and protein expressions were assayed by ELISA. The composition of ginsenosides in 70% ethanol–water extracts of ginseng was analyzed by HPLC. Activation of signaling kinases was examined by Western blot analysis and the respective anti-phospho-protein kinase antibodies. PGSE significantly inhibited the transcription and secretion of CXCL-10 following TNF-α stimulation. Nine ginsenosides including Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg3 and Rh1 were identified in the PGSE by HPLC. Seven out of nine ginsenosides could significantly inhibit TNF-α-induced CXCL-10 expression in U937 cells but the CXCL-10 suppressive effects of individual ginsenosides was less than that of the crude extract or the mixture of reconstituted ginsenosides. The suppressive effect of the reconstituted mixture of nine ginsenosides at the corresponding doses was comparable to the PGSE treatment in a dose-dependent manner. Furthermore, the CXCL-10 suppression can be correlated with the inactivation of ERK1/2 pathways by the PGSE. In summary, our results provide evidence that ginseng can suppress TNF-α-inducible cytokines and signaling proteins in promonocytic cells.
 
dc.descriptionCytokine, 2009, v. 48 n. 1-2, p. 101 abstract no. PP2-046
 
dc.descriptionPoster Presentation II
 
dc.identifier.citationTri-Society Annual Conference 2009 of the Society for Leukocyte Biology, International Cytokine Society & International Society for Interferon and Cytokine Research, Cellular and Cytokine Interactions in Health and Disease, Lisbon, Portugal, 17-21 October, 2009 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.cyto.2009.07.424
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.cyto.2009.07.424
 
dc.identifier.epage101
 
dc.identifier.hkuros168389
 
dc.identifier.isiWOS:000270855100355
 
dc.identifier.issn1043-4666
2012 Impact Factor: 2.518
2012 SCImago Journal Rankings: 0.968
 
dc.identifier.issue1-2
 
dc.identifier.openurl
 
dc.identifier.spage101
 
dc.identifier.urihttp://hdl.handle.net/10722/105734
 
dc.identifier.volume48
 
dc.languageeng
 
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/cytokine
 
dc.relation.ispartofCytokine
 
dc.titleBioactivity-guided identification and cell signaling analysis to investigate the immunomodulatory effects of ginseng on U937 cells
 
dc.typeConference_Paper
 
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<description.abstract>Panax ginseng (Ginseng) is one of the most commonly used medicinal herbs worldwide. It is believed to have beneficial effects against human diseases, and its active components, ginsenosides, may play critical roles in its diverse physiological actions. However, the mechanisms underlying ginseng&#8217;s effects remain to be investigated. We hypothesize that some biological effects of ginseng are due to its anti-inflammatory activities.
To investigate the effects of ginseng on inflammation, human monocytic cells (U937) were treated sequentially with 70% ethanol&#8211;water extracts of Panax ginseng (PGSE) and tumor necrosis factor-alpha (TNF-&#945;). Gene expression profiles of the cells were examined by genechip analysis (Human Genome U133 Plus 2.0 arrays, Affymetrix) and validated by Taqman quantitative RT-PCR, and protein expressions were assayed by ELISA. The composition of ginsenosides in 70% ethanol&#8211;water extracts of ginseng was analyzed by HPLC. Activation of signaling kinases was examined by Western blot analysis and the respective anti-phospho-protein kinase antibodies.
PGSE significantly inhibited the transcription and secretion of CXCL-10 following TNF-&#945; stimulation. Nine ginsenosides including Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg3 and Rh1 were identified in the PGSE by HPLC. Seven out of nine ginsenosides could significantly inhibit TNF-&#945;-induced CXCL-10 expression in U937 cells but the CXCL-10 suppressive effects of individual ginsenosides was less than that of the crude extract or the mixture of reconstituted ginsenosides. The suppressive effect of the reconstituted mixture of nine ginsenosides at the corresponding doses was comparable to the PGSE treatment in a dose-dependent manner. Furthermore, the CXCL-10 suppression can be correlated with the inactivation of ERK1/2 pathways by the PGSE. In summary, our results provide evidence that ginseng can suppress TNF-&#945;-inducible cytokines and signaling proteins in promonocytic cells.</description.abstract>
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