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Conference Paper: Bioactivity-guided identification and cell signaling analysis to investigate the immunomodulatory effects of ginseng on U937 cells

TitleBioactivity-guided identification and cell signaling analysis to investigate the immunomodulatory effects of ginseng on U937 cells
Authors
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/cytokine
Citation
Tri-Society Annual Conference 2009 of the Society for Leukocyte Biology, International Cytokine Society & International Society for Interferon and Cytokine Research, Cellular and Cytokine Interactions in Health and Disease, Lisbon, Portugal, 17-21 October, 2009 How to Cite?
AbstractPanax ginseng (Ginseng) is one of the most commonly used medicinal herbs worldwide. It is believed to have beneficial effects against human diseases, and its active components, ginsenosides, may play critical roles in its diverse physiological actions. However, the mechanisms underlying ginseng’s effects remain to be investigated. We hypothesize that some biological effects of ginseng are due to its anti-inflammatory activities. To investigate the effects of ginseng on inflammation, human monocytic cells (U937) were treated sequentially with 70% ethanol–water extracts of Panax ginseng (PGSE) and tumor necrosis factor-alpha (TNF-α). Gene expression profiles of the cells were examined by genechip analysis (Human Genome U133 Plus 2.0 arrays, Affymetrix) and validated by Taqman quantitative RT-PCR, and protein expressions were assayed by ELISA. The composition of ginsenosides in 70% ethanol–water extracts of ginseng was analyzed by HPLC. Activation of signaling kinases was examined by Western blot analysis and the respective anti-phospho-protein kinase antibodies. PGSE significantly inhibited the transcription and secretion of CXCL-10 following TNF-α stimulation. Nine ginsenosides including Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg3 and Rh1 were identified in the PGSE by HPLC. Seven out of nine ginsenosides could significantly inhibit TNF-α-induced CXCL-10 expression in U937 cells but the CXCL-10 suppressive effects of individual ginsenosides was less than that of the crude extract or the mixture of reconstituted ginsenosides. The suppressive effect of the reconstituted mixture of nine ginsenosides at the corresponding doses was comparable to the PGSE treatment in a dose-dependent manner. Furthermore, the CXCL-10 suppression can be correlated with the inactivation of ERK1/2 pathways by the PGSE. In summary, our results provide evidence that ginseng can suppress TNF-α-inducible cytokines and signaling proteins in promonocytic cells.
DescriptionCytokine, 2009, v. 48 n. 1-2, p. 101 abstract no. PP2-046
Poster Presentation II
Persistent Identifierhttp://hdl.handle.net/10722/105734
ISSN
2014 Impact Factor: 2.664
2014 SCImago Journal Rankings: 1.078
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLee, DCWen_HK
dc.contributor.authorYang, LHen_HK
dc.contributor.authorChik, SCCen_HK
dc.contributor.authorLi, CBen_HK
dc.contributor.authorLau, ASYen_HK
dc.date.accessioned2010-09-25T22:46:30Z-
dc.date.available2010-09-25T22:46:30Z-
dc.date.issued2009en_HK
dc.identifier.citationTri-Society Annual Conference 2009 of the Society for Leukocyte Biology, International Cytokine Society & International Society for Interferon and Cytokine Research, Cellular and Cytokine Interactions in Health and Disease, Lisbon, Portugal, 17-21 October, 2009-
dc.identifier.issn1043-4666-
dc.identifier.urihttp://hdl.handle.net/10722/105734-
dc.descriptionCytokine, 2009, v. 48 n. 1-2, p. 101 abstract no. PP2-046-
dc.descriptionPoster Presentation II-
dc.description.abstractPanax ginseng (Ginseng) is one of the most commonly used medicinal herbs worldwide. It is believed to have beneficial effects against human diseases, and its active components, ginsenosides, may play critical roles in its diverse physiological actions. However, the mechanisms underlying ginseng’s effects remain to be investigated. We hypothesize that some biological effects of ginseng are due to its anti-inflammatory activities. To investigate the effects of ginseng on inflammation, human monocytic cells (U937) were treated sequentially with 70% ethanol–water extracts of Panax ginseng (PGSE) and tumor necrosis factor-alpha (TNF-α). Gene expression profiles of the cells were examined by genechip analysis (Human Genome U133 Plus 2.0 arrays, Affymetrix) and validated by Taqman quantitative RT-PCR, and protein expressions were assayed by ELISA. The composition of ginsenosides in 70% ethanol–water extracts of ginseng was analyzed by HPLC. Activation of signaling kinases was examined by Western blot analysis and the respective anti-phospho-protein kinase antibodies. PGSE significantly inhibited the transcription and secretion of CXCL-10 following TNF-α stimulation. Nine ginsenosides including Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg3 and Rh1 were identified in the PGSE by HPLC. Seven out of nine ginsenosides could significantly inhibit TNF-α-induced CXCL-10 expression in U937 cells but the CXCL-10 suppressive effects of individual ginsenosides was less than that of the crude extract or the mixture of reconstituted ginsenosides. The suppressive effect of the reconstituted mixture of nine ginsenosides at the corresponding doses was comparable to the PGSE treatment in a dose-dependent manner. Furthermore, the CXCL-10 suppression can be correlated with the inactivation of ERK1/2 pathways by the PGSE. In summary, our results provide evidence that ginseng can suppress TNF-α-inducible cytokines and signaling proteins in promonocytic cells.-
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/cytokine-
dc.relation.ispartofCytokineen_HK
dc.titleBioactivity-guided identification and cell signaling analysis to investigate the immunomodulatory effects of ginseng on U937 cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1043-4666&volume=48&issue=1-2&spage=101 abstract no. PP2&epage=046&date=2009&atitle=Bioactivity-guided+identification+and+cell+signaling+analysis+to+investigate+the+immunomodulatory+effects+of+ginseng+on+U937+cells-
dc.identifier.emailLee, DCW: dcwlee@HKUCC-COM.hku.hken_HK
dc.identifier.emailYang, LH: cindy@ust.hken_HK
dc.identifier.emailChik, SCC: chikscc@hkucc.hku.hken_HK
dc.identifier.emailLi, CB: jamesli@graduate.hku.hken_HK
dc.identifier.emailLau, ASY: asylau@hku.hken_HK
dc.identifier.authorityLi, CB=rp00496en_HK
dc.identifier.authorityLau, ASY=rp00474en_HK
dc.identifier.doi10.1016/j.cyto.2009.07.424-
dc.identifier.hkuros168389en_HK
dc.identifier.volume48-
dc.identifier.issue1-2-
dc.identifier.spage101-
dc.identifier.epage101-
dc.identifier.isiWOS:000270855100355-

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