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Conference Paper: Functional Characterization of a Neonatal Heart Expressing-Protein - Analysis of the Protein-interacting Partners of TSPYL-2

TitleFunctional Characterization of a Neonatal Heart Expressing-Protein - Analysis of the Protein-interacting Partners of TSPYL-2
Authors
Issue Date2007
Citation
Joint Meeting of the Japanese Society of Developmental Biologists & the Japan Society for Cell Biology, Fukuoka, Japan, 28-30 May 2007 How to Cite?
AbstractOur group has found a novel gene, Testis-Specific-Protein Y encoded, Like-2 (TSPYL-2), which is expressed in heart. It has been reported that transfection of TSPYL-2 results in growth arrest in HeLa cells, but the exact roles of TSPYL-2 in cell growth are still unclear. To gain insights into the functions of TSPYL-2, we investigate the protein interacting partners of TSPYL-2. In the candidate approach, we found that TSPYL-2 contains a SET/NAP domain which is present in a family of p300/CBP and/or cyclin B1 binding proteins represented by the NAPI and oncoprotein SET. It suggests that p300/CBP and cyclin B1 may interact with TSPYL-2. However, neither of the candidates can co-immunoprecipitated with TSPYL-2. Using the pull-down approach, the transcriptional factor Neuronal Stem Cell Leukemia-2 (NSCL-2), is identified to interact with the N-terminal proline-rich domain of TSPYL-2. Further experiments to confirm the protein interaction by co-immunoprecipitation and mammalian two-hybrid assay are underway. References 1. Chai Z et al., SET-related cell division autoantigen-1 (CDA1) arrests cell growth., J Biol Chem. 2001;276(36):33665-74. 2. Kellogg D et al., Members of the NAP/SET family of proteins interact specifically with B-type cyclins., J Cell Biol. 1995;130(3):661-73. 3. Shikama N et al., Functional interaction between nucleosome assembly proteins and p300/CREB-binding protein family coactivators., Mol Cell Biol. 2000; 20(23):8933-43. 4. Sun G et al., Isolation of differentially expressed genes in human heart tissues., Biochim Biophys Acta. 2002;1588(3):241-6. * This project is partially supported by Edward Sai-Kim Hotung Paediatric Education and Research Fund.
Persistent Identifierhttp://hdl.handle.net/10722/105676

 

DC FieldValueLanguage
dc.contributor.authorYip, KYen_HK
dc.contributor.authorChan, SYen_HK
dc.date.accessioned2010-09-25T22:44:07Z-
dc.date.available2010-09-25T22:44:07Z-
dc.date.issued2007en_HK
dc.identifier.citationJoint Meeting of the Japanese Society of Developmental Biologists & the Japan Society for Cell Biology, Fukuoka, Japan, 28-30 May 2007-
dc.identifier.urihttp://hdl.handle.net/10722/105676-
dc.description.abstractOur group has found a novel gene, Testis-Specific-Protein Y encoded, Like-2 (TSPYL-2), which is expressed in heart. It has been reported that transfection of TSPYL-2 results in growth arrest in HeLa cells, but the exact roles of TSPYL-2 in cell growth are still unclear. To gain insights into the functions of TSPYL-2, we investigate the protein interacting partners of TSPYL-2. In the candidate approach, we found that TSPYL-2 contains a SET/NAP domain which is present in a family of p300/CBP and/or cyclin B1 binding proteins represented by the NAPI and oncoprotein SET. It suggests that p300/CBP and cyclin B1 may interact with TSPYL-2. However, neither of the candidates can co-immunoprecipitated with TSPYL-2. Using the pull-down approach, the transcriptional factor Neuronal Stem Cell Leukemia-2 (NSCL-2), is identified to interact with the N-terminal proline-rich domain of TSPYL-2. Further experiments to confirm the protein interaction by co-immunoprecipitation and mammalian two-hybrid assay are underway. References 1. Chai Z et al., SET-related cell division autoantigen-1 (CDA1) arrests cell growth., J Biol Chem. 2001;276(36):33665-74. 2. Kellogg D et al., Members of the NAP/SET family of proteins interact specifically with B-type cyclins., J Cell Biol. 1995;130(3):661-73. 3. Shikama N et al., Functional interaction between nucleosome assembly proteins and p300/CREB-binding protein family coactivators., Mol Cell Biol. 2000; 20(23):8933-43. 4. Sun G et al., Isolation of differentially expressed genes in human heart tissues., Biochim Biophys Acta. 2002;1588(3):241-6. * This project is partially supported by Edward Sai-Kim Hotung Paediatric Education and Research Fund.-
dc.languageengen_HK
dc.relation.ispartofJoint Meeting of the Japanese Society of Developmental Biologists & the Japan Society for Cell Biologyen_HK
dc.titleFunctional Characterization of a Neonatal Heart Expressing-Protein - Analysis of the Protein-interacting Partners of TSPYL-2en_HK
dc.typeConference_Paperen_HK
dc.identifier.emailYip, KY: h0242819@hkusua.hku.hken_HK
dc.identifier.emailChan, SY: sychan@hkucc.hku.hken_HK
dc.identifier.authorityChan, SY=rp00356en_HK
dc.identifier.hkuros129586en_HK

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