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Conference Paper: Short term and prolonged effects of irradiation on human mesenchymal stem cells

TitleShort term and prolonged effects of irradiation on human mesenchymal stem cells
Authors
Issue Date2006
PublisherKluge Carden Jennings Publishing, Co., Ltd.
Citation
2006 BMT Tandem Meetings, Honolulu, HI, 16-20 February 2006. In Biology of Blood and Marrow Transplantation, 2006, v. 12 n. 2 S1, p. 108 How to Cite?
AbstractBackground: Mesenchymal stem cells (MSCs) are important component of the bone marrow microenvironment and are progenitors of mesenchymal tissues. The effect of therapeutic irradiation to MSCs remains unexplored. Materials and Methods: MSCs were derived from healthy donors. Effects of irradiation with single dose (dosage ranges 2, 4, 6, 8, 12 Gy) on human MSCs were investigated. Post-irradiation cell proliferation was assessed by XTT assay and cell counting. Osteogenic and adipogenic differentiation function were evaluated after irradiation. ALP activity and calcium deposition in irradiated MSCs were quantified following osteogenic induction. Results: Irradiation inhibited proliferation of human MSCs up to 2 weeks post-irradiation but thereafter, survival cells regained normal proliferation rate back to the control level. ALP activity and calcium deposition were both reduced in a dose-dependant fashion. Maximum reduction (16% of control ALP activity and 9% of control calcium level) was noted in MSCs under 12 Gy irradiation. Adipocyte percentage was reduced by 50% in cultures that received irradiation >4 Gy. Attempts to protect the irradiated cells with 1 μM all-trans retinoic acid showed no protective effect on MSCs proliferation and differentiation. Conclusion: Irradiation damaged the capacity of human MSCs proliferation and differentiation. However, full recovery of growth and renewal ability was observed in surviving MSCs after 2 weeks.
Persistent Identifierhttp://hdl.handle.net/10722/105571
ISSN
2015 Impact Factor: 3.98
2015 SCImago Journal Rankings: 2.094

 

DC FieldValueLanguage
dc.contributor.authorLi, Jen_HK
dc.contributor.authorKwong, DLWen_HK
dc.contributor.authorChan, GCFen_HK
dc.date.accessioned2010-09-25T22:39:43Z-
dc.date.available2010-09-25T22:39:43Z-
dc.date.issued2006en_HK
dc.identifier.citation2006 BMT Tandem Meetings, Honolulu, HI, 16-20 February 2006. In Biology of Blood and Marrow Transplantation, 2006, v. 12 n. 2 S1, p. 108en_HK
dc.identifier.issn1083-8791-
dc.identifier.urihttp://hdl.handle.net/10722/105571-
dc.description.abstractBackground: Mesenchymal stem cells (MSCs) are important component of the bone marrow microenvironment and are progenitors of mesenchymal tissues. The effect of therapeutic irradiation to MSCs remains unexplored. Materials and Methods: MSCs were derived from healthy donors. Effects of irradiation with single dose (dosage ranges 2, 4, 6, 8, 12 Gy) on human MSCs were investigated. Post-irradiation cell proliferation was assessed by XTT assay and cell counting. Osteogenic and adipogenic differentiation function were evaluated after irradiation. ALP activity and calcium deposition in irradiated MSCs were quantified following osteogenic induction. Results: Irradiation inhibited proliferation of human MSCs up to 2 weeks post-irradiation but thereafter, survival cells regained normal proliferation rate back to the control level. ALP activity and calcium deposition were both reduced in a dose-dependant fashion. Maximum reduction (16% of control ALP activity and 9% of control calcium level) was noted in MSCs under 12 Gy irradiation. Adipocyte percentage was reduced by 50% in cultures that received irradiation >4 Gy. Attempts to protect the irradiated cells with 1 μM all-trans retinoic acid showed no protective effect on MSCs proliferation and differentiation. Conclusion: Irradiation damaged the capacity of human MSCs proliferation and differentiation. However, full recovery of growth and renewal ability was observed in surviving MSCs after 2 weeks.-
dc.languageengen_HK
dc.publisherKluge Carden Jennings Publishing, Co., Ltd.-
dc.relation.ispartofBiology of Blood and Marrow Transplantationen_HK
dc.titleShort term and prolonged effects of irradiation on human mesenchymal stem cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailKwong, DLW: dlwkwong@hkucc.hku.hken_HK
dc.identifier.emailChan, GCF: gcfchan@hkucc.hku.hken_HK
dc.identifier.authorityKwong, DLW=rp00414en_HK
dc.identifier.authorityChan, GCF=rp00431en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbmt.2005.11.332-
dc.identifier.hkuros116273en_HK
dc.identifier.volume12en_HK
dc.identifier.issue2en_HK
dc.identifier.spage108en_HK

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