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Conference Paper: Changes in the t-tubular system following eccentric muscle damage

TitleChanges in the t-tubular system following eccentric muscle damage
Authors
Issue Date2002
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.acsm-msse.org
Citation
Medicine and Science in Sports and Exercise, v. 34 n. 5 suppl. 1S, p. S204 How to Cite?
AbstractPURPOSE:To examine the morphology and functional status of the T-tubular system following an eccentric contraction protocol. METHODS:Single fibers were dissected from mouse flexor digitorum brevis muscles. The fibers were stretched by 40% of their optimal length for a series of ten tetani. The T-tubules were examined using confocal microscopy and an extracellular fluorescent dye, sulforhodamine B. The time course of the dye washout was used as an estimate of the accessibility of the T-system to the extracellular space. RESULTS:Following eccentric damage, the fibers showed a reduction in force to 31 ± 2.7% of control, a shift in the peak length-tension by 1.24 ± 0.02 and a steepening of the force-frequency relation. This damage was associated with the development of vacuoles connected to the T-tubules. The rate of diffusion of the dye in and out of the T-system was significantly slowed (P < 0.01) with a half-time of 6.32 ± 2.4 min. Ouabain, a sodium pump inhibitor, inhibited the formation of vacuoles suggesting that the sodium pump is essential for their development. CONCLUSION:We propose that in eccentric contractions, T-tubules suffer shearing damage caused by sarcomere inhomogeneities. This leads to a localized increase in intracellular sodium ions. This sodium, accompanied by water, is pumped out of the cell by the sodium pump. the resulting volume load exceeds the capacity of the T-tubules and results in vacuole production.
DescriptionSession - E33C Free Communacation/Slide Muscle Injury
Persistent Identifierhttp://hdl.handle.net/10722/105295
ISSN
2015 Impact Factor: 4.041
2015 SCImago Journal Rankings: 2.007

 

DC FieldValueLanguage
dc.contributor.authorYeung, WEen_HK
dc.contributor.authorBalnave, CDen_HK
dc.contributor.authorBallard, HJen_HK
dc.contributor.authorBourreau, JPen_HK
dc.contributor.authorAllen, DGen_HK
dc.date.accessioned2010-09-25T22:28:08Z-
dc.date.available2010-09-25T22:28:08Z-
dc.date.issued2002en_HK
dc.identifier.citationMedicine and Science in Sports and Exercise, v. 34 n. 5 suppl. 1S, p. S204en_HK
dc.identifier.issn0195-9131en_HK
dc.identifier.urihttp://hdl.handle.net/10722/105295-
dc.descriptionSession - E33C Free Communacation/Slide Muscle Injury-
dc.description.abstractPURPOSE:To examine the morphology and functional status of the T-tubular system following an eccentric contraction protocol. METHODS:Single fibers were dissected from mouse flexor digitorum brevis muscles. The fibers were stretched by 40% of their optimal length for a series of ten tetani. The T-tubules were examined using confocal microscopy and an extracellular fluorescent dye, sulforhodamine B. The time course of the dye washout was used as an estimate of the accessibility of the T-system to the extracellular space. RESULTS:Following eccentric damage, the fibers showed a reduction in force to 31 ± 2.7% of control, a shift in the peak length-tension by 1.24 ± 0.02 and a steepening of the force-frequency relation. This damage was associated with the development of vacuoles connected to the T-tubules. The rate of diffusion of the dye in and out of the T-system was significantly slowed (P < 0.01) with a half-time of 6.32 ± 2.4 min. Ouabain, a sodium pump inhibitor, inhibited the formation of vacuoles suggesting that the sodium pump is essential for their development. CONCLUSION:We propose that in eccentric contractions, T-tubules suffer shearing damage caused by sarcomere inhomogeneities. This leads to a localized increase in intracellular sodium ions. This sodium, accompanied by water, is pumped out of the cell by the sodium pump. the resulting volume load exceeds the capacity of the T-tubules and results in vacuole production.-
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.acsm-msse.orgen_HK
dc.relation.ispartofMedicine and Science in Sports and Exerciseen_HK
dc.rightsMedicine and Science in Sports and Exercise. Copyright © Lippincott Williams & Wilkins.en_HK
dc.titleChanges in the t-tubular system following eccentric muscle damageen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0195-9131&volume=34 &issue=5&spage=S204&epage=&date=2002&atitle=Changes+in+the+t-tubular+system+following+eccentric+muscle+damageen_HK
dc.identifier.emailBallard, HJ: ballard@hkucc.hku.hken_HK
dc.identifier.emailBourreau, JP: bourreau@hkucc.hku.hken_HK
dc.identifier.authorityBallard, HJ=rp00367en_HK
dc.identifier.authorityBourreau, JP=rp00389en_HK
dc.identifier.hkuros72936en_HK
dc.identifier.volume34en_HK
dc.identifier.issue5 suppl. 1Sen_HK
dc.identifier.spageS204en_HK
dc.identifier.epageS204-

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