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Conference Paper: Neurokinin receptor one agonist septide offers neuroprotection on dopaminergic neurons
Title | Neurokinin receptor one agonist septide offers neuroprotection on dopaminergic neurons |
---|---|
Authors | |
Issue Date | 2006 |
Publisher | S Karger AG. The Journal's web site is located at http://www.karger.com/NSG |
Citation | The 25th Annual Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December 2005. In Neurosignals, 2006, v. 15 n. 3, p. 140 How to Cite? |
Abstract | Parkinson’s disease is a serious motor disorder and it is caused
by a degeneration of dopaminergic neurons in the substantia nigra
pars compacta. Neurokinins (NK) are a group of neuropeptides
that are suggested to be involved in the pathogenesis of Parkinson’s
disease. Functions of NKs are mediated by NK receptors.
Substance P, the natural ligand of NK1 receptor, is found to have
neuroprotective effects on dopaminergic neurons. Septide is a selective
NK1 receptor agonist. In order to investigate the neuroprotective
effect of septide in vitro, septide (1 M) was co-incubated
with 6-hydroxydopamine (6-OHDA, 200 M) in primary cell
cultures of neonatal rat dopaminergic neurons. After 25 h, massive
neuronal cell death was observed in those cultures incubated
with 6-OHDA, whereas in septide co-incubation cultures most
neurons were seen to be intact. By flow cytometric analysis, 17.03
8 2.13% of tyrosine hydroxylase (TH)-immunoreactive neurons
were found to survive after co-incubation treatment but only 4.92
8 1.40% of TH-positive neurons were found to survive after 6-
OHDA treatment. In addition, double immunofluorescence revealed
that the level of TH immunoreactivity was also reduced in
the surviving neurons after 6-OHDA treatment. No significant
reduction of TH immunoreactivity was found in neurons co-incubated
with septide and 6-OHDA. The present results indicate
that septide has neuroprotective effects on dopaminergic neurons
in culture. In addition, in rat model of Parkinson’s disease, neuroroprotective effects on dopaminergic neurons were also observed.
Activation of NK1 receptor by septide may have implications in
treatment of Parkinson’s disease.
Acknowledgement: The present work was supported by Joint
Research Scheme, National Natural Science Foundation of
China and Research Grants Council of Hong Kong, 30218002
and N_HKBU202/02. |
Persistent Identifier | http://hdl.handle.net/10722/105229 |
ISSN | 2016 Impact Factor: 6.143 2023 SCImago Journal Rankings: 0.458 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chan, WS | en_HK |
dc.contributor.author | Chen, L | en_HK |
dc.contributor.author | Chan, YS | en_HK |
dc.contributor.author | Yung, KK | en_HK |
dc.date.accessioned | 2010-09-25T22:25:26Z | - |
dc.date.available | 2010-09-25T22:25:26Z | - |
dc.date.issued | 2006 | en_HK |
dc.identifier.citation | The 25th Annual Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December 2005. In Neurosignals, 2006, v. 15 n. 3, p. 140 | en_HK |
dc.identifier.issn | 1424-862X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/105229 | - |
dc.description.abstract | Parkinson’s disease is a serious motor disorder and it is caused by a degeneration of dopaminergic neurons in the substantia nigra pars compacta. Neurokinins (NK) are a group of neuropeptides that are suggested to be involved in the pathogenesis of Parkinson’s disease. Functions of NKs are mediated by NK receptors. Substance P, the natural ligand of NK1 receptor, is found to have neuroprotective effects on dopaminergic neurons. Septide is a selective NK1 receptor agonist. In order to investigate the neuroprotective effect of septide in vitro, septide (1 M) was co-incubated with 6-hydroxydopamine (6-OHDA, 200 M) in primary cell cultures of neonatal rat dopaminergic neurons. After 25 h, massive neuronal cell death was observed in those cultures incubated with 6-OHDA, whereas in septide co-incubation cultures most neurons were seen to be intact. By flow cytometric analysis, 17.03 8 2.13% of tyrosine hydroxylase (TH)-immunoreactive neurons were found to survive after co-incubation treatment but only 4.92 8 1.40% of TH-positive neurons were found to survive after 6- OHDA treatment. In addition, double immunofluorescence revealed that the level of TH immunoreactivity was also reduced in the surviving neurons after 6-OHDA treatment. No significant reduction of TH immunoreactivity was found in neurons co-incubated with septide and 6-OHDA. The present results indicate that septide has neuroprotective effects on dopaminergic neurons in culture. In addition, in rat model of Parkinson’s disease, neuroroprotective effects on dopaminergic neurons were also observed. Activation of NK1 receptor by septide may have implications in treatment of Parkinson’s disease. Acknowledgement: The present work was supported by Joint Research Scheme, National Natural Science Foundation of China and Research Grants Council of Hong Kong, 30218002 and N_HKBU202/02. | - |
dc.language | eng | en_HK |
dc.publisher | S Karger AG. The Journal's web site is located at http://www.karger.com/NSG | en_HK |
dc.relation.ispartof | Neurosignals | en_HK |
dc.rights | Neurosignals. Copyright © S Karger AG. | en_HK |
dc.title | Neurokinin receptor one agonist septide offers neuroprotection on dopaminergic neurons | en_HK |
dc.type | Conference_Paper | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1424-862X&volume=15&spage=140&epage=&date=2006&atitle=Neurokinin+receptor+one+agonist+septide+offers+neuroprotection+on+dopaminergic+neurons | en_HK |
dc.identifier.email | Chan, YS: yschan@hkucc.hku.hk | en_HK |
dc.identifier.authority | Chan, YS=rp00318 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1159/000095356 | - |
dc.identifier.hkuros | 137700 | en_HK |
dc.identifier.volume | 15 | en_HK |
dc.identifier.spage | 140 | en_HK |
dc.identifier.issnl | 1424-862X | - |