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Conference Paper: Neurokinin receptor one agonist septide offers neuroprotection on dopaminergic neurons

TitleNeurokinin receptor one agonist septide offers neuroprotection on dopaminergic neurons
Authors
Issue Date2006
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
The 25th Annual Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December 2005. In Neurosignals, 2006, v. 15 n. 3, p. 140 How to Cite?
AbstractParkinson’s disease is a serious motor disorder and it is caused by a degeneration of dopaminergic neurons in the substantia nigra pars compacta. Neurokinins (NK) are a group of neuropeptides that are suggested to be involved in the pathogenesis of Parkinson’s disease. Functions of NKs are mediated by NK receptors. Substance P, the natural ligand of NK1 receptor, is found to have neuroprotective effects on dopaminergic neurons. Septide is a selective NK1 receptor agonist. In order to investigate the neuroprotective effect of septide in vitro, septide (1 M) was co-incubated with 6-hydroxydopamine (6-OHDA, 200 M) in primary cell cultures of neonatal rat dopaminergic neurons. After 25 h, massive neuronal cell death was observed in those cultures incubated with 6-OHDA, whereas in septide co-incubation cultures most neurons were seen to be intact. By flow cytometric analysis, 17.03 8 2.13% of tyrosine hydroxylase (TH)-immunoreactive neurons were found to survive after co-incubation treatment but only 4.92 8 1.40% of TH-positive neurons were found to survive after 6- OHDA treatment. In addition, double immunofluorescence revealed that the level of TH immunoreactivity was also reduced in the surviving neurons after 6-OHDA treatment. No significant reduction of TH immunoreactivity was found in neurons co-incubated with septide and 6-OHDA. The present results indicate that septide has neuroprotective effects on dopaminergic neurons in culture. In addition, in rat model of Parkinson’s disease, neuroroprotective effects on dopaminergic neurons were also observed. Activation of NK1 receptor by septide may have implications in treatment of Parkinson’s disease. Acknowledgement: The present work was supported by Joint Research Scheme, National Natural Science Foundation of China and Research Grants Council of Hong Kong, 30218002 and N_HKBU202/02.
Persistent Identifierhttp://hdl.handle.net/10722/105229
ISSN
2015 Impact Factor: 1.593
2015 SCImago Journal Rankings: 0.763

 

DC FieldValueLanguage
dc.contributor.authorChan, WSen_HK
dc.contributor.authorChen, Len_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorYung, KKen_HK
dc.date.accessioned2010-09-25T22:25:26Z-
dc.date.available2010-09-25T22:25:26Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 25th Annual Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December 2005. In Neurosignals, 2006, v. 15 n. 3, p. 140en_HK
dc.identifier.issn1424-862Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/105229-
dc.description.abstractParkinson’s disease is a serious motor disorder and it is caused by a degeneration of dopaminergic neurons in the substantia nigra pars compacta. Neurokinins (NK) are a group of neuropeptides that are suggested to be involved in the pathogenesis of Parkinson’s disease. Functions of NKs are mediated by NK receptors. Substance P, the natural ligand of NK1 receptor, is found to have neuroprotective effects on dopaminergic neurons. Septide is a selective NK1 receptor agonist. In order to investigate the neuroprotective effect of septide in vitro, septide (1 M) was co-incubated with 6-hydroxydopamine (6-OHDA, 200 M) in primary cell cultures of neonatal rat dopaminergic neurons. After 25 h, massive neuronal cell death was observed in those cultures incubated with 6-OHDA, whereas in septide co-incubation cultures most neurons were seen to be intact. By flow cytometric analysis, 17.03 8 2.13% of tyrosine hydroxylase (TH)-immunoreactive neurons were found to survive after co-incubation treatment but only 4.92 8 1.40% of TH-positive neurons were found to survive after 6- OHDA treatment. In addition, double immunofluorescence revealed that the level of TH immunoreactivity was also reduced in the surviving neurons after 6-OHDA treatment. No significant reduction of TH immunoreactivity was found in neurons co-incubated with septide and 6-OHDA. The present results indicate that septide has neuroprotective effects on dopaminergic neurons in culture. In addition, in rat model of Parkinson’s disease, neuroroprotective effects on dopaminergic neurons were also observed. Activation of NK1 receptor by septide may have implications in treatment of Parkinson’s disease. Acknowledgement: The present work was supported by Joint Research Scheme, National Natural Science Foundation of China and Research Grants Council of Hong Kong, 30218002 and N_HKBU202/02.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_HK
dc.relation.ispartofNeurosignalsen_HK
dc.rightsNeurosignals. Copyright © S Karger AG.en_HK
dc.titleNeurokinin receptor one agonist septide offers neuroprotection on dopaminergic neuronsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1424-862X&volume=15&spage=140&epage=&date=2006&atitle=Neurokinin+receptor+one+agonist+septide+offers+neuroprotection+on+dopaminergic+neuronsen_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000095356-
dc.identifier.hkuros137700en_HK
dc.identifier.volume15en_HK
dc.identifier.spage140en_HK

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