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Conference Paper: The effects of ageing on the responses in HIF-1 binding and adrenomedullin gene expression in the rat lung to acute hypoxia

TitleThe effects of ageing on the responses in HIF-1 binding and adrenomedullin gene expression in the rat lung to acute hypoxia
Authors
Issue Date2004
PublisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/npep
Citation
The 2004 Joint International Symposium on Calcitonin Gene-related Peptide, Amylin and Calcitonin; 4th Symposium on Adrenomedullin and Proadrenomedullin N-20 Peptide, Zurich, Switzerland, 18-20 March 2004. In Neuropeptides (Edinburgh), 2004, v. 38 n. 2-3, p. 125-126, abstract no. P15 How to Cite?
AbstractAdrenomedullin (AM) may play an important role in the physiological response to hypoxia, particularly in the lung where it may cause bronchiodilation and vasodilation. Hypoxia-induced transcription of the gene coding for AM is regulated by the hypoxia-inducible factor-1 (HIF-1) and recent studies have shown a decrease of HIF-1 protein and its DNA binding activity in old animals. The objective of this study is to find out whether the AM response to acute hypoxia is compromised in the old rats. Male S.D. rats aged 3 months (young), 11–12 months (middle-aged) and 22 months (old) were subjected to acute hypoxia of 8% oxygen for 6 h and the binding of HIF-1 to DNA and the levels and gene expression of AM were measured in the lung and compared with age matched controls. There was an age-related increase in HIF-1 binding to DNA and acute hypoxia led to an increase of this binding in the young and middle-aged but not in the old rats. The preproAM mRNA and AM levels in the lung did not show any age-related difference but the response to acute hypoxia was absent in the old rats. Immunoctytochemical studies of the lung from the young rat demonstrated AM in the epithelial cells of the bronchioles, alveolar cells and also in macrophages. There were increases in plasma AM levels in response to hypoxia in rats of all 3 ages as well as preproAM mRNA levels in other tissues. In conclusion, ageing may affect the response of the AM system in the lung and other tissues in response to acute hypoxia.
Persistent Identifierhttp://hdl.handle.net/10722/105198
ISSN
2015 Impact Factor: 2.726
2015 SCImago Journal Rankings: 1.165

 

DC FieldValueLanguage
dc.contributor.authorTang, Fen_HK
dc.contributor.authorHwang, ISSen_HK
dc.contributor.authorWong, PFen_HK
dc.contributor.authorLiong, ECen_HK
dc.contributor.authorFung, MLen_HK
dc.date.accessioned2010-09-25T22:24:11Z-
dc.date.available2010-09-25T22:24:11Z-
dc.date.issued2004en_HK
dc.identifier.citationThe 2004 Joint International Symposium on Calcitonin Gene-related Peptide, Amylin and Calcitonin; 4th Symposium on Adrenomedullin and Proadrenomedullin N-20 Peptide, Zurich, Switzerland, 18-20 March 2004. In Neuropeptides (Edinburgh), 2004, v. 38 n. 2-3, p. 125-126, abstract no. P15en_HK
dc.identifier.issn0143-4179-
dc.identifier.urihttp://hdl.handle.net/10722/105198-
dc.description.abstractAdrenomedullin (AM) may play an important role in the physiological response to hypoxia, particularly in the lung where it may cause bronchiodilation and vasodilation. Hypoxia-induced transcription of the gene coding for AM is regulated by the hypoxia-inducible factor-1 (HIF-1) and recent studies have shown a decrease of HIF-1 protein and its DNA binding activity in old animals. The objective of this study is to find out whether the AM response to acute hypoxia is compromised in the old rats. Male S.D. rats aged 3 months (young), 11–12 months (middle-aged) and 22 months (old) were subjected to acute hypoxia of 8% oxygen for 6 h and the binding of HIF-1 to DNA and the levels and gene expression of AM were measured in the lung and compared with age matched controls. There was an age-related increase in HIF-1 binding to DNA and acute hypoxia led to an increase of this binding in the young and middle-aged but not in the old rats. The preproAM mRNA and AM levels in the lung did not show any age-related difference but the response to acute hypoxia was absent in the old rats. Immunoctytochemical studies of the lung from the young rat demonstrated AM in the epithelial cells of the bronchioles, alveolar cells and also in macrophages. There were increases in plasma AM levels in response to hypoxia in rats of all 3 ages as well as preproAM mRNA levels in other tissues. In conclusion, ageing may affect the response of the AM system in the lung and other tissues in response to acute hypoxia.-
dc.languageengen_HK
dc.publisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/npep-
dc.relation.ispartofNeuropeptides (Edinburgh)en_HK
dc.rights© 2004..This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.titleThe effects of ageing on the responses in HIF-1 binding and adrenomedullin gene expression in the rat lung to acute hypoxiaen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.emailHwang, ISS: isabelbelbel@hotmail.comen_HK
dc.identifier.emailLiong, EC: eclionga@HKUCC.hku.hken_HK
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.identifier.authorityFung, ML=rp00433en_HK
dc.identifier.doi10.1016/j.npep.2004.01.003-
dc.identifier.hkuros88083en_HK
dc.identifier.hkuros104337-
dc.identifier.volume38-
dc.identifier.issue2-3-
dc.identifier.spage125, abstract no. P15-
dc.identifier.epage126-
dc.publisher.placeUnited Kingdom-

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