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Conference Paper: Increase in interstitial ATP of rat soleus muscle during lactic acid infusion

TitleIncrease in interstitial ATP of rat soleus muscle during lactic acid infusion
Authors
Issue Date2007
PublisherFederation of American Societies for Experimental Biology.
Citation
The 2007 Meeting of Experimental Biology (EB 2007), Washington, DC., 28 April-2 May 2007. In The FASEB Journal, 2007, v. 21 n. 5, p. A483, abstract no. 586.17 How to Cite?
AbstractWe investigated the effect of acidosis on ATP efflux from muscle. Lactic acid (LA) was infused into the arterial supply of the buffer-perfused rat hindlimb muscle; ATP concentrations were measured by HPLC in interstitial microdialysate from soleus muscle. Inhibitors of several ATP transporters were used to explore the mechanism. LA (10 mM) reduced venous pH from 7.14±0.02 to 7.00±0.03 (P<0.01) and increased interstitial ATP from 31±6 nM to 62±10 nM (P<0.01, n=13). Infusion of alpha-cyano-4-hydroxycinnamic acid, a lactate-transporter-inhibitor, prevented the LA-infusion-induced increase in interstitial ATP, suggesting that cellular uptake of the LA is necessary for the stimulation of ATP efflux. LA-induced elevation of interstitial ATP was reduced to 48±5 nM by nifedipine, an inhibitor of electrodiffusional transport, and abolished by the nonspecific blockers of CFTR, 4-acetamido-4′-isothiocyanatostilbene-2, 2′-disulfonic acid (SITS, 100 μM) or glibenclamide (200 μM). Atractyloside, an inhibitor of mitochondrial ATP transporter, could not prevent LA-infusion-stimulated ATP efflux. These results suggested that pH depression could stimulate the efflux of ATP from rat soleus muscle, thereby elevating the interstitial ATP concentration. This ATP release had the nature of electrodiffusional movement, and may be conducted through CFTR. This study is supported by the University of Hong Kong and the Hong Kong Research Grants Council.
Persistent Identifierhttp://hdl.handle.net/10722/105189
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorTu, Jen_HK
dc.contributor.authorBallard, HJen_HK
dc.date.accessioned2010-09-25T22:23:49Z-
dc.date.available2010-09-25T22:23:49Z-
dc.date.issued2007en_HK
dc.identifier.citationThe 2007 Meeting of Experimental Biology (EB 2007), Washington, DC., 28 April-2 May 2007. In The FASEB Journal, 2007, v. 21 n. 5, p. A483, abstract no. 586.17en_HK
dc.identifier.issn0892-6638en_HK
dc.identifier.urihttp://hdl.handle.net/10722/105189-
dc.description.abstractWe investigated the effect of acidosis on ATP efflux from muscle. Lactic acid (LA) was infused into the arterial supply of the buffer-perfused rat hindlimb muscle; ATP concentrations were measured by HPLC in interstitial microdialysate from soleus muscle. Inhibitors of several ATP transporters were used to explore the mechanism. LA (10 mM) reduced venous pH from 7.14±0.02 to 7.00±0.03 (P<0.01) and increased interstitial ATP from 31±6 nM to 62±10 nM (P<0.01, n=13). Infusion of alpha-cyano-4-hydroxycinnamic acid, a lactate-transporter-inhibitor, prevented the LA-infusion-induced increase in interstitial ATP, suggesting that cellular uptake of the LA is necessary for the stimulation of ATP efflux. LA-induced elevation of interstitial ATP was reduced to 48±5 nM by nifedipine, an inhibitor of electrodiffusional transport, and abolished by the nonspecific blockers of CFTR, 4-acetamido-4′-isothiocyanatostilbene-2, 2′-disulfonic acid (SITS, 100 μM) or glibenclamide (200 μM). Atractyloside, an inhibitor of mitochondrial ATP transporter, could not prevent LA-infusion-stimulated ATP efflux. These results suggested that pH depression could stimulate the efflux of ATP from rat soleus muscle, thereby elevating the interstitial ATP concentration. This ATP release had the nature of electrodiffusional movement, and may be conducted through CFTR. This study is supported by the University of Hong Kong and the Hong Kong Research Grants Council.-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology.en_HK
dc.relation.ispartofThe FASEB Journalen_HK
dc.titleIncrease in interstitial ATP of rat soleus muscle during lactic acid infusionen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0892-6638&volume=21&spage=586.17&epage=&date=2007&atitle=Increase+in+interstitial+ATP+of+rat+soleus+muscle+during+lactic+acid+infusion.en_HK
dc.identifier.emailBallard, HJ: ballard@hkucc.hku.hken_HK
dc.identifier.authorityBallard, HJ=rp00367en_HK
dc.identifier.hkuros136141en_HK
dc.identifier.volume21en_HK
dc.identifier.issue5-
dc.identifier.spageA483, abstract no. 586.17-
dc.identifier.epageA483, abstract no. 586.17-

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