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Conference Paper: Localization, characterization and subtyping of melatonin receptors in rat uterine endometrium in estrous stage

TitleLocalization, characterization and subtyping of melatonin receptors in rat uterine endometrium in estrous stage
Authors
Issue Date1999
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
The 1999 Annual Physiology Symposium, Hong Kong, China, 21-22 May 1999. In Biological Signals and Receptors, 1999, v. 8 n. 3, p. 218-219 How to Cite?
AbstractThis study was undertaken to identify the rat uterine melatonin receptors. Autoradiography showed that 2-[125I]iodomelatonin binding were localized in the rat uterine endometrial stroma. The specific binding of 2-[125I]iodomelatonin was stable, saturable, reversible and of high affinity. Scatchard analysis of the 2-[125I]iodomelatonin binding in membrane preparations of rat uterine endometrium in estrous stage had an equilibrium dissociation constant (Kd) of 28.9 B 3.59 pmol/l (n = 8) and a maximum number of binding sites (Bmax) of 1.6 B 0.15 fmol/mg protein (n = 8). The Kd value determined from kinetic analysis was 16.5 B 3.02 (n = 3). Competition studies using various indoles and neurotransmitters demonstrated that 2-iodomelatonin, melatonin, 6-chloromelatonin, 6-hydroxymelatonin, N-acetylserotonin showed significant inhibition of the 2-[125I]iodomelatonin binding, while the other compounds had no significant inhibition. Nucleotide sequencing demonstrated that MEL1A but not MEL1B receptor mRNAs were expressed in uterine endometrium in estrous stage after reverse transcription-Polymerase chain reactions (RT-PCR) and nested PCR. Our results indicate the presence of MEL1A receptor subtype in the rat uterine endometrial stroma.
Descriptionpp. 193–220 of this journal entitled: Annual Physiology Symposium 1999
Persistent Identifierhttp://hdl.handle.net/10722/105183
ISSN
2003 Impact Factor: 3.5

 

DC FieldValueLanguage
dc.contributor.authorZhao, Hen_HK
dc.contributor.authorPoon, AMSen_HK
dc.contributor.authorPang, SFen_HK
dc.date.accessioned2010-09-25T22:23:34Z-
dc.date.available2010-09-25T22:23:34Z-
dc.date.issued1999en_HK
dc.identifier.citationThe 1999 Annual Physiology Symposium, Hong Kong, China, 21-22 May 1999. In Biological Signals and Receptors, 1999, v. 8 n. 3, p. 218-219-
dc.identifier.issn1422-4933en_HK
dc.identifier.urihttp://hdl.handle.net/10722/105183-
dc.descriptionpp. 193–220 of this journal entitled: Annual Physiology Symposium 1999-
dc.description.abstractThis study was undertaken to identify the rat uterine melatonin receptors. Autoradiography showed that 2-[125I]iodomelatonin binding were localized in the rat uterine endometrial stroma. The specific binding of 2-[125I]iodomelatonin was stable, saturable, reversible and of high affinity. Scatchard analysis of the 2-[125I]iodomelatonin binding in membrane preparations of rat uterine endometrium in estrous stage had an equilibrium dissociation constant (Kd) of 28.9 B 3.59 pmol/l (n = 8) and a maximum number of binding sites (Bmax) of 1.6 B 0.15 fmol/mg protein (n = 8). The Kd value determined from kinetic analysis was 16.5 B 3.02 (n = 3). Competition studies using various indoles and neurotransmitters demonstrated that 2-iodomelatonin, melatonin, 6-chloromelatonin, 6-hydroxymelatonin, N-acetylserotonin showed significant inhibition of the 2-[125I]iodomelatonin binding, while the other compounds had no significant inhibition. Nucleotide sequencing demonstrated that MEL1A but not MEL1B receptor mRNAs were expressed in uterine endometrium in estrous stage after reverse transcription-Polymerase chain reactions (RT-PCR) and nested PCR. Our results indicate the presence of MEL1A receptor subtype in the rat uterine endometrial stroma.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_HK
dc.relation.ispartofBiological Signals and Receptorsen_HK
dc.rightsBiological Signals and Receptors. Copyright © S Karger AG.en_HK
dc.titleLocalization, characterization and subtyping of melatonin receptors in rat uterine endometrium in estrous stageen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1422-4933&volume=8&spage=218&epage=&date=1999&atitle=Localization,+Characterization+and+Subtyping+of+Melatonin+Receptors+in+Rat+Uterine+Endometrium+in+Estrous+Stageen_HK
dc.identifier.emailPoon, AMS: amspoon@hkucc.hku.hken_HK
dc.identifier.emailPang, SF: hrmypsf@hkucc.hku.hken_HK
dc.identifier.authorityPoon, AMS=rp00354en_HK
dc.identifier.doi10.1159/000014590-
dc.identifier.hkuros44284en_HK
dc.identifier.volume8en_HK
dc.identifier.issue3-
dc.identifier.spage218en_HK
dc.identifier.epage219-
dc.publisher.placeSwitzerland-

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