File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Conference Paper: Expression of Fos protein in the medial geniculate body of cochlear-ablated and cortical-activated rats

TitleExpression of Fos protein in the medial geniculate body of cochlear-ablated and cortical-activated rats
Authors
Issue Date2006
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
The Hong Kong Society of Neurosciences 24th Annual Scientific Meeting, Hong Kong, 13-14 January 2005. In Neurosignals, 2006, v. 15 n. 3, p. 128-129 How to Cite?
AbstractThe time course and extent of the expression of Fos protein in neurons within the medial geniculate body (MGB) were examined after chemical activation of the auditory cortex in cochlearablated Sprague-Dawley rats. Surgical ablation of the bilateral cochlea was performed in ketamine-anesthetized animals which were then allowed to recover for 4 h (Group I), 24 h (II), 7 days (III), 14 days (IV), and 30 days (V). After each of these time points, the rats were again anesthetized with ketamine. The exposed auditory cortex on the right side was administered with GABA A antagonist biccuculline methobromide (20 m M ), which in turn resulted in the activation of cells in the injected site. At 1 h after injection, the brain was processed for Fos immunostaining. Completeness of cochlea destruction was ascertained by post-mortem examination of the temporal bone. Control experiments were also conducted in cochlear-ablated animals without the injection of biccuculline. Neuronal activation was defined by Fos expression in cell nuclei. In Group-I rats, Fos labeling was mainly confined within the ventral and medial nuclei of the MGB ipsilateral to the activated hemisphere. In Group-II rats, Fos expression was found in the ventral, medial and dorsal nuclei of the MGB ipsilateral to the activated hemisphere. In Group-III rats, Fos expression was mainly located in the dorsal and medial nuclei, with some in the marginal zone of the MGB, but absent in the ventral nucleus of the MGB. In Groups-IV and V rats, the distribution of Fos-labeled neurons in the MGB was similar to that of Group-III rats but the Fos expression was less intense. We demonstrate that corticofugal induction of neuronal excitability in the non-lemniscal MGB shows a slower time course than in the lemniscal MGB, suggesting that these two areas of the auditory thalamus are differentially modulated by corticofugal pathways.
Persistent Identifierhttp://hdl.handle.net/10722/105126
ISSN
2015 Impact Factor: 1.593
2015 SCImago Journal Rankings: 0.763

 

DC FieldValueLanguage
dc.contributor.authorSun, Xen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorHe, Jen_HK
dc.date.accessioned2010-09-25T22:21:15Z-
dc.date.available2010-09-25T22:21:15Z-
dc.date.issued2006en_HK
dc.identifier.citationThe Hong Kong Society of Neurosciences 24th Annual Scientific Meeting, Hong Kong, 13-14 January 2005. In Neurosignals, 2006, v. 15 n. 3, p. 128-129en_HK
dc.identifier.issn1424-862Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/105126-
dc.description.abstractThe time course and extent of the expression of Fos protein in neurons within the medial geniculate body (MGB) were examined after chemical activation of the auditory cortex in cochlearablated Sprague-Dawley rats. Surgical ablation of the bilateral cochlea was performed in ketamine-anesthetized animals which were then allowed to recover for 4 h (Group I), 24 h (II), 7 days (III), 14 days (IV), and 30 days (V). After each of these time points, the rats were again anesthetized with ketamine. The exposed auditory cortex on the right side was administered with GABA A antagonist biccuculline methobromide (20 m M ), which in turn resulted in the activation of cells in the injected site. At 1 h after injection, the brain was processed for Fos immunostaining. Completeness of cochlea destruction was ascertained by post-mortem examination of the temporal bone. Control experiments were also conducted in cochlear-ablated animals without the injection of biccuculline. Neuronal activation was defined by Fos expression in cell nuclei. In Group-I rats, Fos labeling was mainly confined within the ventral and medial nuclei of the MGB ipsilateral to the activated hemisphere. In Group-II rats, Fos expression was found in the ventral, medial and dorsal nuclei of the MGB ipsilateral to the activated hemisphere. In Group-III rats, Fos expression was mainly located in the dorsal and medial nuclei, with some in the marginal zone of the MGB, but absent in the ventral nucleus of the MGB. In Groups-IV and V rats, the distribution of Fos-labeled neurons in the MGB was similar to that of Group-III rats but the Fos expression was less intense. We demonstrate that corticofugal induction of neuronal excitability in the non-lemniscal MGB shows a slower time course than in the lemniscal MGB, suggesting that these two areas of the auditory thalamus are differentially modulated by corticofugal pathways.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_HK
dc.relation.ispartofNeurosignalsen_HK
dc.rightsNeurosignals. Copyright © S Karger AG.en_HK
dc.titleExpression of Fos protein in the medial geniculate body of cochlear-ablated and cortical-activated ratsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1424-862X&volume=15&spage=128&epage=129&date=2006&atitle=Expression+of+Fos+protein+in+the+medial+geniculate+body+of+cochlear-ablated+and+cortical-activated+ratsen_HK
dc.identifier.emailSun, X: sunxiasun@msn.comen_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000095356-
dc.identifier.hkuros137696en_HK
dc.identifier.volume15en_HK
dc.identifier.spage128en_HK
dc.identifier.epage129en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats