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Conference Paper: Changes in proliferation and apoptosis of human choriocarcinoma JAr and prostate cancer LNCaP cells induced by polysaccharide peptide (PSP) of Yun Zhi

TitleChanges in proliferation and apoptosis of human choriocarcinoma JAr and prostate cancer LNCaP cells induced by polysaccharide peptide (PSP) of Yun Zhi
Authors
Issue Date1999
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
The 1999 Annual Physiology Symposium, Hong Kong, China, 21-22 May 1999. In Biological Signals and Receptors, 1999, v. 8 n. 3, p. 215-216 How to Cite?
AbstractThe effects of polysaccharide peptide (PSP) of Yun Zhi on proliferation and apoptosis of human choriocarcinoma JAr cells and prostate cancer LNCaP cells were investigated. Human JAr cells and LNCaP cells were respectively incubated for 24 h and 48 h in serum-free medium without and with PSP. PSP induced a decrease of thymidine incorporation into Jar and LNCaP cells in a concentration-dependent manner. Significant (p ! 0.01) decreases in thymidine incorporation into JAr cells and LNCaP cells were observed when the cells had been incubated with 0.32, 0.64, 1.28 or 2.56 mg/ml of PSP. Maximal inhibition of JAr cell proliferation to 13.8% of vehicle-treated control was recorded when PSP concentration was 2.56 mg/ml, whereas, the same concentration of PSP induced maximal inhibition of LNCaP cell proliferation to 49.1% of control. For apoptosis studies, Jar and LNCaP cells were incubated for 48 h in serum-free medium without and with PSP. After 48 h incubation, histone-complexed DNA fragments (mono- and oligonucleosomes) in the cytoplasm of apoptotic JAr cells and LNCaP cells were quantitated using the immunoassay kit, Cell Death Detection ELISA Plus (Roche Molecular Biochemicals). While high concentrations of PSP (1.28 and 2.56 mg/ml) induced significant (p ! 0.05) increase in JAr cell apoptosis, both low (0.32 and 0.64 mg/ml) and high (1.28 and 2.56 mg/ml) concen-trations of PSP were found to induce significant (p < 0.01) increase in LNCaP cell apoptosis. Our data support both anti-proliferative and apoptosis-inducing actions of PSP on human choriocarcinoma and prostate cancer cells in vitro. This work was supported by the TCM Research Fund of the Faculty of Medicine, The University of Hong Kong.
Descriptionpp. 193–220 of this journal issue entitled: Annual Physiology Symposium 1999, Hong Kong, China, May 21–22, 1999
Persistent Identifierhttp://hdl.handle.net/10722/105045
ISSN
2003 Impact Factor: 3.5

 

DC FieldValueLanguage
dc.contributor.authorXi, SCen_HK
dc.contributor.authorLi, Len_HK
dc.contributor.authorShiu, SYWen_HK
dc.date.accessioned2010-09-25T22:17:59Z-
dc.date.available2010-09-25T22:17:59Z-
dc.date.issued1999en_HK
dc.identifier.citationThe 1999 Annual Physiology Symposium, Hong Kong, China, 21-22 May 1999. In Biological Signals and Receptors, 1999, v. 8 n. 3, p. 215-216en_HK
dc.identifier.issn1422-4933en_HK
dc.identifier.urihttp://hdl.handle.net/10722/105045-
dc.descriptionpp. 193–220 of this journal issue entitled: Annual Physiology Symposium 1999, Hong Kong, China, May 21–22, 1999-
dc.description.abstractThe effects of polysaccharide peptide (PSP) of Yun Zhi on proliferation and apoptosis of human choriocarcinoma JAr cells and prostate cancer LNCaP cells were investigated. Human JAr cells and LNCaP cells were respectively incubated for 24 h and 48 h in serum-free medium without and with PSP. PSP induced a decrease of thymidine incorporation into Jar and LNCaP cells in a concentration-dependent manner. Significant (p ! 0.01) decreases in thymidine incorporation into JAr cells and LNCaP cells were observed when the cells had been incubated with 0.32, 0.64, 1.28 or 2.56 mg/ml of PSP. Maximal inhibition of JAr cell proliferation to 13.8% of vehicle-treated control was recorded when PSP concentration was 2.56 mg/ml, whereas, the same concentration of PSP induced maximal inhibition of LNCaP cell proliferation to 49.1% of control. For apoptosis studies, Jar and LNCaP cells were incubated for 48 h in serum-free medium without and with PSP. After 48 h incubation, histone-complexed DNA fragments (mono- and oligonucleosomes) in the cytoplasm of apoptotic JAr cells and LNCaP cells were quantitated using the immunoassay kit, Cell Death Detection ELISA Plus (Roche Molecular Biochemicals). While high concentrations of PSP (1.28 and 2.56 mg/ml) induced significant (p ! 0.05) increase in JAr cell apoptosis, both low (0.32 and 0.64 mg/ml) and high (1.28 and 2.56 mg/ml) concen-trations of PSP were found to induce significant (p < 0.01) increase in LNCaP cell apoptosis. Our data support both anti-proliferative and apoptosis-inducing actions of PSP on human choriocarcinoma and prostate cancer cells in vitro. This work was supported by the TCM Research Fund of the Faculty of Medicine, The University of Hong Kong.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_HK
dc.relation.ispartofBiological Signals and Receptorsen_HK
dc.rightsBiological Signals and Receptors. Copyright © S Karger AG.en_HK
dc.titleChanges in proliferation and apoptosis of human choriocarcinoma JAr and prostate cancer LNCaP cells induced by polysaccharide peptide (PSP) of Yun Zhien_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1422-4933&volume=8&spage=215&epage=&date=1999&atitle=Changes+in+proliferation+and+apoptosis+of+human+choriocarcinoma+JAr+and+prostate+cancer+LNCaP+cells+induced+by+polysaccharide+peptide+(PSP)+of+Yun+Zhien_HK
dc.identifier.emailShiu, SYW: sywshiu@hkucc.hku.hken_HK
dc.identifier.authorityShiu, SYW=rp00384en_HK
dc.identifier.doi10.1159/000014590-
dc.identifier.hkuros43598en_HK
dc.identifier.volume8en_HK
dc.identifier.issue3-
dc.identifier.spage215en_HK

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