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Conference Paper: Neuroprotective effects of neurokinin receptor one agonist septide in dopaminergic neurons in primary culture

TitleNeuroprotective effects of neurokinin receptor one agonist septide in dopaminergic neurons in primary culture
Authors
Keywords6-hydroxydopamine
septide
dopaminergic neurons
Neurokinin receptor
Issue Date2005
PublisherSociety for Neuroscience
Citation
Neuroscience 2005, Washington, DC, 12-16 November 2005, Presentation no. 211.7 How to Cite?
AbstractParkinson´s disease is a serious motor disorder and it is caused by a degeneration of dopaminergic neurons in the substantia nigra pars compacta. Neurokinins (NK) are a group of neuropeptides that are suggested to be involved in the pathogenesis of Parkinson´s disease. Functions of NKs are mediated by NK receptors. Substance P, the natural ligand of NK1 receptor, is found to have neuroprotective effects on dopaminergic neurons. Septide is a selective NK1 receptor agonist. In order to investigate the neuroprotective effect of septide in vitro, septide (1uM) was co-incubated with 6-hydroxydopamine (6-OHDA, 200uM) in primary cell cultures of neonatal rat dopaminergic neurons. After 25 h, massive neuronal cell death was observed in those cultures incubated with 6-OHDA, whereas in septide co-incubation cultures most neurons were seen to be intact. By flow cytometric analysis, 17.03 ± 2.13 % of tyrosine hydroxylase (TH)-immunoreactive neurons were found to survive after co-incubation treatment but only 4.92 ± 1.40 % of TH-positive neurons were found to survive after 6-OHDA treatment. In addition, double immunofluorescence revealed that the level of TH immunoreactivity was also reduced in the surviving neurons after 6-OHDA treatment. No significant reduction of TH immunoreactivity was found in neurons co-incubated with septide and 6-OHDA. The present results indicate that septide has neuroprotective effects on dopaminergic neurons in culture. Activation of NK1 receptor by septide may have implications in treatment of Parkinson´s disease. Supported by Joint Research Scheme, National Natural Science Foundation of China and Research Grants Council of Hong Kong, 30218002 and N_HKBU202/02
Persistent Identifierhttp://hdl.handle.net/10722/104998

 

DC FieldValueLanguage
dc.contributor.authorChan, WSen_HK
dc.contributor.authorChen, LWen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorYung, KKLen_HK
dc.date.accessioned2010-09-25T22:16:05Z-
dc.date.available2010-09-25T22:16:05Z-
dc.date.issued2005en_HK
dc.identifier.citationNeuroscience 2005, Washington, DC, 12-16 November 2005, Presentation no. 211.7en_HK
dc.identifier.urihttp://hdl.handle.net/10722/104998-
dc.description.abstractParkinson´s disease is a serious motor disorder and it is caused by a degeneration of dopaminergic neurons in the substantia nigra pars compacta. Neurokinins (NK) are a group of neuropeptides that are suggested to be involved in the pathogenesis of Parkinson´s disease. Functions of NKs are mediated by NK receptors. Substance P, the natural ligand of NK1 receptor, is found to have neuroprotective effects on dopaminergic neurons. Septide is a selective NK1 receptor agonist. In order to investigate the neuroprotective effect of septide in vitro, septide (1uM) was co-incubated with 6-hydroxydopamine (6-OHDA, 200uM) in primary cell cultures of neonatal rat dopaminergic neurons. After 25 h, massive neuronal cell death was observed in those cultures incubated with 6-OHDA, whereas in septide co-incubation cultures most neurons were seen to be intact. By flow cytometric analysis, 17.03 ± 2.13 % of tyrosine hydroxylase (TH)-immunoreactive neurons were found to survive after co-incubation treatment but only 4.92 ± 1.40 % of TH-positive neurons were found to survive after 6-OHDA treatment. In addition, double immunofluorescence revealed that the level of TH immunoreactivity was also reduced in the surviving neurons after 6-OHDA treatment. No significant reduction of TH immunoreactivity was found in neurons co-incubated with septide and 6-OHDA. The present results indicate that septide has neuroprotective effects on dopaminergic neurons in culture. Activation of NK1 receptor by septide may have implications in treatment of Parkinson´s disease. Supported by Joint Research Scheme, National Natural Science Foundation of China and Research Grants Council of Hong Kong, 30218002 and N_HKBU202/02-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meetingen_HK
dc.subject6-hydroxydopamine-
dc.subjectseptide-
dc.subjectdopaminergic neurons-
dc.subjectNeurokinin receptor-
dc.titleNeuroprotective effects of neurokinin receptor one agonist septide in dopaminergic neurons in primary cultureen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.identifier.hkuros121792en_HK

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