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Conference Paper: Expression and action of secretin in rat cerebellar deep nuclei neurons

TitleExpression and action of secretin in rat cerebellar deep nuclei neurons
Authors
Issue Date2006
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
The Hong Kong Society of Neurosciences 24th Annual Scientific Meeting, Hong Kong, 13-14 January 2005. In Neurosignals, 2006, v. 15 n. 3, p. 118-119(OP-13/24) How to Cite?
AbstractWe recently reported that secretin and its receptors are expressed in the cerebellum of the rat [1] and human [2] . Electrophysiologically, secretin modulates the inhibitory neurotransmission from basket cells to Purkinje neurons. However, despite the fact that Purkinje neurons express secretin receptor mRNA, secretin administration does not evoke any postsynaptic responses in these cells. These observations suggest that Purkinje neurons may synthesize secretin receptors and transport them to nerve terminals in the deep nuclei. To test this hypothesis, we examined the expression of secretin and secretin receptors in the cerebellar deep nuclei of rats and studied the electrophysiological actions of secretin on GABAergic neurotransmission from Purkinje neurons to deep nuclei neurons. By means of in situ hybridization, deep nuclei neurons were found to express both secretin and secretin receptor mRNA. In agreement with this observation, immunohistochemical staining showed strong expression of secretin in the same type of neurons. Furthermore, administration of 30 n M of secretin increased the frequency of TTX-resistant, or miniature, inhibitory postsynaptic currents by 61.6% in 14 cells (p ! 0.05) while the amplitudes were not affected. These data suggest that secretin released by deep nuclei neurons activate presynaptic secretin receptors on the terminals of Purkinje neurons. Therefore, secretin appears to modulate the activity of the cerebellum by acting at multiple levels of inhibitory synapses in the cerebellar circuit. Acknowledgement: Supported by the National Natural Science Foundation of China and the Research Grants Council of Hong Kong (N_CUHK 427/03).
Persistent Identifierhttp://hdl.handle.net/10722/104990
ISSN
2015 Impact Factor: 1.593
2015 SCImago Journal Rankings: 0.763

 

DC FieldValueLanguage
dc.contributor.authorYung, WHen_HK
dc.contributor.authorLee, MYen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorWang, JJen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-09-25T22:15:46Z-
dc.date.available2010-09-25T22:15:46Z-
dc.date.issued2006en_HK
dc.identifier.citationThe Hong Kong Society of Neurosciences 24th Annual Scientific Meeting, Hong Kong, 13-14 January 2005. In Neurosignals, 2006, v. 15 n. 3, p. 118-119(OP-13/24)en_HK
dc.identifier.issn1424-862Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/104990-
dc.description.abstractWe recently reported that secretin and its receptors are expressed in the cerebellum of the rat [1] and human [2] . Electrophysiologically, secretin modulates the inhibitory neurotransmission from basket cells to Purkinje neurons. However, despite the fact that Purkinje neurons express secretin receptor mRNA, secretin administration does not evoke any postsynaptic responses in these cells. These observations suggest that Purkinje neurons may synthesize secretin receptors and transport them to nerve terminals in the deep nuclei. To test this hypothesis, we examined the expression of secretin and secretin receptors in the cerebellar deep nuclei of rats and studied the electrophysiological actions of secretin on GABAergic neurotransmission from Purkinje neurons to deep nuclei neurons. By means of in situ hybridization, deep nuclei neurons were found to express both secretin and secretin receptor mRNA. In agreement with this observation, immunohistochemical staining showed strong expression of secretin in the same type of neurons. Furthermore, administration of 30 n M of secretin increased the frequency of TTX-resistant, or miniature, inhibitory postsynaptic currents by 61.6% in 14 cells (p ! 0.05) while the amplitudes were not affected. These data suggest that secretin released by deep nuclei neurons activate presynaptic secretin receptors on the terminals of Purkinje neurons. Therefore, secretin appears to modulate the activity of the cerebellum by acting at multiple levels of inhibitory synapses in the cerebellar circuit. Acknowledgement: Supported by the National Natural Science Foundation of China and the Research Grants Council of Hong Kong (N_CUHK 427/03).-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_HK
dc.relation.ispartofNeurosignalsen_HK
dc.rightsNeurosignals. Copyright © S Karger AG.en_HK
dc.titleExpression and action of secretin in rat cerebellar deep nuclei neuronsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLee, MY: myleesuki01@yahoo.com.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hk-
dc.identifier.emailChow, BKC: bkcc@hkusua.hku.hk-
dc.identifier.authorityLee, MY=rp01536en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000095356-
dc.identifier.hkuros137686-
dc.identifier.hkuros136181-
dc.identifier.volume15en_HK
dc.identifier.issue3-
dc.identifier.spage118 OP-13/24en_HK
dc.identifier.epage119 OP-13/24en_HK
dc.publisher.placeSwitzerland-
dc.description.otherThe 24th and the 25th Scientific Meeting of the Hong Kong Society of Neurosciences, Hong Kong, 5-6 December 2005. In Neurosignals, 2006, v. 15 n. 3, p. 118-119(OP-13/24)-

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