File Download

There are no files associated with this item.

Supplementary

Conference Paper: Developmental responsiveness of rat dorsomedial cell column neurons to horizontal and vertical linear acceleration

TitleDevelopmental responsiveness of rat dorsomedial cell column neurons to horizontal and vertical linear acceleration
Authors
KeywordsINFERIOR OLIVE
GLUTAMATE RECEPTOR
MATURATION
VESTIBULAR
Issue Date2004
PublisherSociety for Neuroscience
Citation
Neuroscience 2004, San Diego, CA, 23-27 October 2004, Presentation no. 652.4 How to Cite?
AbstractTo chart the postnatal time course by which 3-D spatial reference of gravity information is encoded in the dorsomedial cell column (DMCC) of the inferior olive, conscious Sprague-Dawley rats (P6–adult) were subjected to linear acceleration along the vertical or horizontal plane. Neuronal activation was discerned by the expression of Fos protein in cell nuclei of the DMCC. Fos-labeled neurons were rarely found in control rats. In experimental rats, DMCC neurons that showed c-fos expression in response to vertical stimulation were observed first at P9 while those to horizontal interaural stimulation were observed from P11 onwards. No functionally activated DMCC neuron was found with horizontal stimulation along the antero-posterior axis. Extracellular recordings in decerebrate rats subjected to otolith stimulation on the horizontal plane also confirmed that the best response orientations of DMCC neurons were confined to the transverse direction. Altogether, these indicate that otolith-related DMCC neurons only process information about head movements in the coronal plane. The expression profile of ionotropic glutamate receptor subunits in these developing neurons was also examined by combining immuno- and hybridization histochemistry experiments. In each age group studied, about 95-98% of Fos-labeled neurons responsive to horizontal interaural stimulation or vertical stimulation expressed NMDA (NR1, NR2A, NR2B) or AMPA (GluR2/3) receptor subunits. While the granule density of both NR2A and NR2B mRNAs decreased from P6 onwards, that of NR1 mRNA increased with age, peaked at P11 and reached the adult level by P21. Our findings suggest that dynamic changes in the expression of glutamate receptor subunits contribute to the maturation of otolith-related DMCC neurons in the coding of head movements in the coronal plane. Supported by HK RGC
Persistent Identifierhttp://hdl.handle.net/10722/104953

 

DC FieldValueLanguage
dc.contributor.authorLi, Cen_HK
dc.contributor.authorLai, SKen_HK
dc.contributor.authorZhou, Hen_HK
dc.contributor.authorLiu, Jen_HK
dc.contributor.authorLai, CHen_HK
dc.contributor.authorChan, YSen_HK
dc.date.accessioned2010-09-25T22:14:16Z-
dc.date.available2010-09-25T22:14:16Z-
dc.date.issued2004en_HK
dc.identifier.citationNeuroscience 2004, San Diego, CA, 23-27 October 2004, Presentation no. 652.4en_HK
dc.identifier.urihttp://hdl.handle.net/10722/104953-
dc.description.abstractTo chart the postnatal time course by which 3-D spatial reference of gravity information is encoded in the dorsomedial cell column (DMCC) of the inferior olive, conscious Sprague-Dawley rats (P6–adult) were subjected to linear acceleration along the vertical or horizontal plane. Neuronal activation was discerned by the expression of Fos protein in cell nuclei of the DMCC. Fos-labeled neurons were rarely found in control rats. In experimental rats, DMCC neurons that showed c-fos expression in response to vertical stimulation were observed first at P9 while those to horizontal interaural stimulation were observed from P11 onwards. No functionally activated DMCC neuron was found with horizontal stimulation along the antero-posterior axis. Extracellular recordings in decerebrate rats subjected to otolith stimulation on the horizontal plane also confirmed that the best response orientations of DMCC neurons were confined to the transverse direction. Altogether, these indicate that otolith-related DMCC neurons only process information about head movements in the coronal plane. The expression profile of ionotropic glutamate receptor subunits in these developing neurons was also examined by combining immuno- and hybridization histochemistry experiments. In each age group studied, about 95-98% of Fos-labeled neurons responsive to horizontal interaural stimulation or vertical stimulation expressed NMDA (NR1, NR2A, NR2B) or AMPA (GluR2/3) receptor subunits. While the granule density of both NR2A and NR2B mRNAs decreased from P6 onwards, that of NR1 mRNA increased with age, peaked at P11 and reached the adult level by P21. Our findings suggest that dynamic changes in the expression of glutamate receptor subunits contribute to the maturation of otolith-related DMCC neurons in the coding of head movements in the coronal plane. Supported by HK RGC-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meetingen_HK
dc.subjectINFERIOR OLIVE-
dc.subjectGLUTAMATE RECEPTOR-
dc.subjectMATURATION-
dc.subjectVESTIBULAR-
dc.titleDevelopmental responsiveness of rat dorsomedial cell column neurons to horizontal and vertical linear accelerationen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLi, C: lic5@hkusua.hku.hken_HK
dc.identifier.emailLai, SK: estherlai@hkusua.hku.hken_HK
dc.identifier.emailZhou, H: zhouhua_01@163.comen_HK
dc.identifier.emailLiu, J: liuj@hkusua.hku.hken_HK
dc.identifier.emailLai, CH: chlaib@HKUSUA.hku.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityLai, CH=rp00396en_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.identifier.hkuros107338en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats