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Conference Paper: Adenomedullin expression and cytokine response in experimentally induced endotoxaemia

TitleAdenomedullin expression and cytokine response in experimentally induced endotoxaemia
Authors
Issue Date2004
PublisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/npep
Citation
The 2004 Joint International Symposium on Calcitonin Gene-related Peptide, Amylin and Calcitonin; 4th Symposium on Adrenomedullin and Proadrenomedullin N-20 Peptide, Zurich, Switzerland, 18-20 March 2004. In Neuropeptides (Edinburgh), 2004, v. 38 n. 2-3, p. 126, abstract no. P16 How to Cite?
AbstractBACKGROUND: Adrenomedullin (AM) is a potent vasodilatory peptide involved in cardiovascular homeostasis and in inflammation. To study the effect of septicaemia, we examined the temporal changes in tissue AM and preproAM mRNA levels, and cytokine response in a rat model of endotoxaemia. METHOD: Male Sprague–Dawley rats received intraperitoneal injection of lipopolysaccharide (LPS) at 10 mg/kg weight, or saline as control. Rats were sacrificed at 1, 3, 6, 12 and 24 h after injection. Concentrations of AM and proinflammatory cytokines (TNF-a, IL-1b and IL-6) in the plasma and various tissues were measured. PreproAM mRNA levels were determined using solution hybridization-RNase protection assay. RESULTS: Plasma AM was raised at 3, 6, and 12 h. There were significant increases in preproAM mRNA levels in the lung at 3 and 6 h, in the liver at 3 h, and in the kidney at 1 and 3 h; while there was no change in preproAM mRNA levels in the spleen and adrenal gland. Immunoreactive AM concentration was increased in the lung at 3, 6, and 12 h, but not changed in the liver or the kidney. Concentrations of TNF-a were raised in the plasma at 1, 3 and 6 h, in the lung at 1 h, in the spleen at 1–3 h, and the liver at 1 h. Concentrations of IL-1b were increased in the plasma at 3 and 6 h, in the lung at 3–12 h, in the spleen at 1–12 h, and the kidney at 6 h. Concentrations of IL-6 were increased in the plasma at 3–12 h. However, there was no significant change in IL-6 levels in the tissue extracts tested. CONCLUSIONS: Endotoxin stimulates the expression of AM and proinflammatory cytokines in systemic circulatory and localized tissues. AM may be involved in the systemic response to sepsis.
Persistent Identifierhttp://hdl.handle.net/10722/104929
ISSN
2015 Impact Factor: 2.726
2015 SCImago Journal Rankings: 1.165

 

DC FieldValueLanguage
dc.contributor.authorCheung, BMYen_HK
dc.contributor.authorWong, LYFen_HK
dc.contributor.authorLi, CYYen_HK
dc.contributor.authorHwang, ISSen_HK
dc.contributor.authorTang, F-
dc.date.accessioned2010-09-25T22:13:17Z-
dc.date.available2010-09-25T22:13:17Z-
dc.date.issued2004en_HK
dc.identifier.citationThe 2004 Joint International Symposium on Calcitonin Gene-related Peptide, Amylin and Calcitonin; 4th Symposium on Adrenomedullin and Proadrenomedullin N-20 Peptide, Zurich, Switzerland, 18-20 March 2004. In Neuropeptides (Edinburgh), 2004, v. 38 n. 2-3, p. 126, abstract no. P16-
dc.identifier.issn0143-4179-
dc.identifier.urihttp://hdl.handle.net/10722/104929-
dc.description.abstractBACKGROUND: Adrenomedullin (AM) is a potent vasodilatory peptide involved in cardiovascular homeostasis and in inflammation. To study the effect of septicaemia, we examined the temporal changes in tissue AM and preproAM mRNA levels, and cytokine response in a rat model of endotoxaemia. METHOD: Male Sprague–Dawley rats received intraperitoneal injection of lipopolysaccharide (LPS) at 10 mg/kg weight, or saline as control. Rats were sacrificed at 1, 3, 6, 12 and 24 h after injection. Concentrations of AM and proinflammatory cytokines (TNF-a, IL-1b and IL-6) in the plasma and various tissues were measured. PreproAM mRNA levels were determined using solution hybridization-RNase protection assay. RESULTS: Plasma AM was raised at 3, 6, and 12 h. There were significant increases in preproAM mRNA levels in the lung at 3 and 6 h, in the liver at 3 h, and in the kidney at 1 and 3 h; while there was no change in preproAM mRNA levels in the spleen and adrenal gland. Immunoreactive AM concentration was increased in the lung at 3, 6, and 12 h, but not changed in the liver or the kidney. Concentrations of TNF-a were raised in the plasma at 1, 3 and 6 h, in the lung at 1 h, in the spleen at 1–3 h, and the liver at 1 h. Concentrations of IL-1b were increased in the plasma at 3 and 6 h, in the lung at 3–12 h, in the spleen at 1–12 h, and the kidney at 6 h. Concentrations of IL-6 were increased in the plasma at 3–12 h. However, there was no significant change in IL-6 levels in the tissue extracts tested. CONCLUSIONS: Endotoxin stimulates the expression of AM and proinflammatory cytokines in systemic circulatory and localized tissues. AM may be involved in the systemic response to sepsis.-
dc.languageengen_HK
dc.publisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/npep-
dc.relation.ispartofNeuropeptides (Edinburgh)en_HK
dc.rights© 2004. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.titleAdenomedullin expression and cytokine response in experimentally induced endotoxaemiaen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailCheung, BMY: mycheung@hku.hken_HK
dc.identifier.emailLi, YY: cyyli@graduate.hku.hken_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.identifier.doi10.1016/j.npep.2004.01.003-
dc.identifier.hkuros104354en_HK
dc.identifier.volume38-
dc.identifier.issue2-3-
dc.identifier.spage126, abstract no. P16-
dc.identifier.epage126, abstract no. P16-

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