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Conference Paper: Gene expression of the melatonin receptor in human granulosa cells

TitleGene expression of the melatonin receptor in human granulosa cells
Authors
Issue Date1998
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
The 1997 Annual Physiology Symposium, Tainan, Taiwan, 17-19 October 1997. In Biological Signals and Receptors, 1998, v. 7 n. 5, p. 299 How to Cite?
AbstractRecent findings have shown that melatonin emerges as an effective oral contraceptive and that it appears to be a novel alternative for fertility control in humans because anovulation is achieved without the many negative side-effects associated with estrogencontaining oral contraceptives. In view of the putative presence of melatonin receptors in the ovary, as determined y binding studies, and the high concentration of melatonin in follicular fluid in comparison to plasma, a direct inhibitory effect on ovarian function is supported. The mechanism underlying anovulation by melatonin is unknown but the demonstration of melatonin receptors in human granulosa cells points to an autocrine regulatory role for melatonin. Human granulosa cells were aspirated from preovulatory follicles from women undergoing in vitro fertilization. After isolation of total cytosolic ribonucleic acid (RNA) from the cultured cells, they were reverse-transcribed to first strand complementary deoxyribonucleic acid (cDNA) and amplified with a set of primers derived from the human melatonin 1b (Mel1b) receptor cDNA clone. Preliminary studies show that the polymerase chain reaction product corresponded to the expected size of the Mel1b receptor. This finding provides evidence for the gene expression of the Mel1b receptor in human granulosa cells and suggests that melatonin is a regulator in the ovary.
DescriptionThis journal issue entitled: Physiology Symposium - Annual Symposium, Hong Kong, April 1998 / Tainan, October 1997: Abstracts
pp. 286–304: Annual Physiology Symposium 1997, Tainan, Taiwan, October 17–19, 1997
Persistent Identifierhttp://hdl.handle.net/10722/104916
ISSN
2003 Impact Factor: 3.5

 

DC FieldValueLanguage
dc.contributor.authorWoo, MMMen_HK
dc.contributor.authorShiu, SYWen_HK
dc.contributor.authorPang, SFen_HK
dc.date.accessioned2010-09-25T22:12:45Z-
dc.date.available2010-09-25T22:12:45Z-
dc.date.issued1998en_HK
dc.identifier.citationThe 1997 Annual Physiology Symposium, Tainan, Taiwan, 17-19 October 1997. In Biological Signals and Receptors, 1998, v. 7 n. 5, p. 299en_HK
dc.identifier.issn1422-4933en_HK
dc.identifier.urihttp://hdl.handle.net/10722/104916-
dc.descriptionThis journal issue entitled: Physiology Symposium - Annual Symposium, Hong Kong, April 1998 / Tainan, October 1997: Abstracts-
dc.descriptionpp. 286–304: Annual Physiology Symposium 1997, Tainan, Taiwan, October 17–19, 1997-
dc.description.abstractRecent findings have shown that melatonin emerges as an effective oral contraceptive and that it appears to be a novel alternative for fertility control in humans because anovulation is achieved without the many negative side-effects associated with estrogencontaining oral contraceptives. In view of the putative presence of melatonin receptors in the ovary, as determined y binding studies, and the high concentration of melatonin in follicular fluid in comparison to plasma, a direct inhibitory effect on ovarian function is supported. The mechanism underlying anovulation by melatonin is unknown but the demonstration of melatonin receptors in human granulosa cells points to an autocrine regulatory role for melatonin. Human granulosa cells were aspirated from preovulatory follicles from women undergoing in vitro fertilization. After isolation of total cytosolic ribonucleic acid (RNA) from the cultured cells, they were reverse-transcribed to first strand complementary deoxyribonucleic acid (cDNA) and amplified with a set of primers derived from the human melatonin 1b (Mel1b) receptor cDNA clone. Preliminary studies show that the polymerase chain reaction product corresponded to the expected size of the Mel1b receptor. This finding provides evidence for the gene expression of the Mel1b receptor in human granulosa cells and suggests that melatonin is a regulator in the ovary.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_HK
dc.relation.ispartofBiological Signals and Receptorsen_HK
dc.rightsBiological Signals and Receptors. Copyright © S Karger AG.en_HK
dc.titleGene expression of the melatonin receptor in human granulosa cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1422-4933&volume=7&spage=299&epage=&date=1998&atitle=Gene+expression+of+the+melatonin+receptor+in+human+granulosa+cellsen_HK
dc.identifier.emailShiu, SYW: sywshiu@hkucc.hku.hken_HK
dc.identifier.emailPang, SF: hrmypsf@hkucc.hku.hken_HK
dc.identifier.authorityShiu, SYW=rp00384en_HK
dc.identifier.doi10.1159/000014552-
dc.identifier.hkuros43524en_HK
dc.identifier.volume7en_HK
dc.identifier.issue5-
dc.identifier.spage299en_HK

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