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Conference Paper: Phosphoantigen-expanded Human Gammadelta T Cells Display Potent Cytotoxicities Towards Human and Avian Influenza Virus-infected Monocyte-derived Macrophages

TitlePhosphoantigen-expanded Human Gammadelta T Cells Display Potent Cytotoxicities Towards Human and Avian Influenza Virus-infected Monocyte-derived Macrophages
Authors
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yclim
Citation
The 9th Annual Meeting of the Federation of Clinical Immunology Societies (FOCIS 2009), San Fransisco, CA., 11-15 June 2009. In Clinical Immunology, 2009, v. 131 suppl. 1, p. S91-S92 How to Cite?
AbstractBackground. Influenza virus is a cause of substantial annual morbidity and mortality worldwide. Potential for the emergence of a new pandemic strain, e.g. avian influenza virus, is a major concern. Currently available vaccines and anti-influenza drugs have limited effectiveness for influenza infections, especially for new pandemic strains. Therefore, there is an acute need for developing alternative strategies for influenza therapy. γδ-T cells have potent antiviral activities against different viruses. However, no data are available for their antiviral activity against influenza viruses. Methods. In this study, we used virus-infected primary human monocyte-derived macrophages (MDMs) to examine the antiviral activity of phosphoantigen isopentenyl pyrophosphate (IPP)-expanded human Vγ9Vδ2-T cells against influenza viruses. Results. Vγ9Vδ2-T cells were selectively activated and expanded by IPP from peripheral blood mononuclear cells. IPP-expanded Vγ9Vδ2-T cells efficiently killed human (H1N1) and avian (H9N2 and H5N1) influenza virus-infected MDMs and significantly inhibited viral replication. The cytotoxicity of Vγ9Vδ2-T cells against influenza virus-infected MDMs was dependent on NKG2D activation, and mediated by Fas-FasL and perforin-granzyme B pathways. Conclusion. Our study suggests a potentially novel therapeutic approach for seasonal, zoonotic avian and pandemic influenza by using phosphoantigens to activate γδ-T cells against influenza infections.
Persistent Identifierhttp://hdl.handle.net/10722/103238
ISSN
2015 Impact Factor: 4.034
2015 SCImago Journal Rankings: 1.848

 

DC FieldValueLanguage
dc.contributor.authorQin, G-
dc.contributor.authorMao, H-
dc.contributor.authorZheng, J-
dc.contributor.authorSia, SF-
dc.contributor.authorLiu, Y-
dc.contributor.authorChan, PL-
dc.contributor.authorLam, KT-
dc.contributor.authorPeiris, JSM-
dc.contributor.authorLau, YL-
dc.contributor.authorTu, W-
dc.date.accessioned2010-09-25T21:03:57Z-
dc.date.available2010-09-25T21:03:57Z-
dc.date.issued2009-
dc.identifier.citationThe 9th Annual Meeting of the Federation of Clinical Immunology Societies (FOCIS 2009), San Fransisco, CA., 11-15 June 2009. In Clinical Immunology, 2009, v. 131 suppl. 1, p. S91-S92-
dc.identifier.issn1521-6616-
dc.identifier.urihttp://hdl.handle.net/10722/103238-
dc.description.abstractBackground. Influenza virus is a cause of substantial annual morbidity and mortality worldwide. Potential for the emergence of a new pandemic strain, e.g. avian influenza virus, is a major concern. Currently available vaccines and anti-influenza drugs have limited effectiveness for influenza infections, especially for new pandemic strains. Therefore, there is an acute need for developing alternative strategies for influenza therapy. γδ-T cells have potent antiviral activities against different viruses. However, no data are available for their antiviral activity against influenza viruses. Methods. In this study, we used virus-infected primary human monocyte-derived macrophages (MDMs) to examine the antiviral activity of phosphoantigen isopentenyl pyrophosphate (IPP)-expanded human Vγ9Vδ2-T cells against influenza viruses. Results. Vγ9Vδ2-T cells were selectively activated and expanded by IPP from peripheral blood mononuclear cells. IPP-expanded Vγ9Vδ2-T cells efficiently killed human (H1N1) and avian (H9N2 and H5N1) influenza virus-infected MDMs and significantly inhibited viral replication. The cytotoxicity of Vγ9Vδ2-T cells against influenza virus-infected MDMs was dependent on NKG2D activation, and mediated by Fas-FasL and perforin-granzyme B pathways. Conclusion. Our study suggests a potentially novel therapeutic approach for seasonal, zoonotic avian and pandemic influenza by using phosphoantigens to activate γδ-T cells against influenza infections.-
dc.languageeng-
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yclim-
dc.relation.ispartofClinical Immunology-
dc.rights© 2009. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.titlePhosphoantigen-expanded Human Gammadelta T Cells Display Potent Cytotoxicities Towards Human and Avian Influenza Virus-infected Monocyte-derived Macrophages-
dc.typeConference_Paper-
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1521-6616&volume=131&spage=s91&epage=s92&date=2009&atitle=Phosphoantigen-expanded+Human+Gammadelta+T+Cells+Display+Potent+Cytotoxicities+Towards+Human+and+Avian+Influenza+Virus-infected+Monocyte-derived+Macrophagesen_HK
dc.identifier.emailSia, SF: sfsia@HKUCC.hku.hk-
dc.identifier.emailLiu, Y: yinpingl@hku.hk-
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hk-
dc.identifier.emailLau, YL: lauylung@hkucc.hku.hk-
dc.identifier.emailTu, W: wwtu@yahoo.com-
dc.identifier.authorityLiu, Y=rp00269-
dc.identifier.authorityPeiris, JSM=rp00410-
dc.identifier.authorityLau, YL=rp00361-
dc.identifier.authorityTu, W=rp00416-
dc.identifier.doi10.1016/j.clim.2009.03.268-
dc.identifier.hkuros163414-
dc.identifier.volume131-
dc.identifier.issuesuppl. 1-
dc.identifier.spageS91-
dc.identifier.epageS92-
dc.publisher.placeUnited States-

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