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Conference Paper: Functional characterization of a novel nucleoporin gene nup98 in zebrafish embryos

TitleFunctional characterization of a novel nucleoporin gene nup98 in zebrafish embryos
Authors
Issue Date2009
PublisherThe University of Hong Kong.
Citation
The 16th Hong Kong International Cancer Congresss and 6th Annual Meeting Centre for Cancer Research, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 4-6 November 2009. How to Cite?
AbstractBackground: The nucleoporin gene nup98 is important for the regulation of cytoplasmic-nuclear trafficking. Frequent disruptions of NUP98 during chromosomal translocation in acute myeloid leukemia suggest that it may play a role in normal hematopoiesis. nup98-knockout mice has resulted in early embryonic lethality. Therefore, its role in embryonic hematopoiesis remains unclear. In this study, we have cloned a zebrafish nup98 gene and examined its role in embryonic development, with particular reference to hematopoiesis. Methods: Two expressed sequence tags with translated sequence homologous to human NUP98 were identified. The gene was cloned by PCR from cDNA of zebrafish embryos. Expression of nup98 in zebrafish embryos was investigated spatially by whole-mount in-situ hybridization and temporally by RT-PCR. The functions of nup98 were examined by morpholino knockdown and the effects on embryonic development evaluated by gene expression studies and confocal microscopy. Cellular functions of zebrafish nup98 were investigated in HeLa cells. Results: Zebrafish nup98 gene shared 65% identity to human NUP98 homolog in protein sequence. The gene was expressed during early embryonic development since 1-cell stage and diffusely in eyes and the developing brain since 18 hpf. About 30% nup98-knockdown embryos developed intracranial hemorrhage at 48 hpf, resulting from disrupted blood vessels. nup98-knockdown upregulated pu.1 and scl as evaluated by quantitative RT-PCR. Moreover, ectopic expression of zebrafish nup98 rescued the defective mRNA export due to NUP98 knockdown in HeLa cells. Conclusion: A novel zebrafish nup98 gene was shown to exhibit conserved function in mRNA trafficking. Its role in embryonic development should be further evaluated. 26
Persistent Identifierhttp://hdl.handle.net/10722/103004

 

DC FieldValueLanguage
dc.contributor.authorFung, TKen_HK
dc.contributor.authorLeung, AYHen_HK
dc.date.accessioned2010-09-25T20:53:40Z-
dc.date.available2010-09-25T20:53:40Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 16th Hong Kong International Cancer Congresss and 6th Annual Meeting Centre for Cancer Research, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 4-6 November 2009.-
dc.identifier.urihttp://hdl.handle.net/10722/103004-
dc.description.abstractBackground: The nucleoporin gene nup98 is important for the regulation of cytoplasmic-nuclear trafficking. Frequent disruptions of NUP98 during chromosomal translocation in acute myeloid leukemia suggest that it may play a role in normal hematopoiesis. nup98-knockout mice has resulted in early embryonic lethality. Therefore, its role in embryonic hematopoiesis remains unclear. In this study, we have cloned a zebrafish nup98 gene and examined its role in embryonic development, with particular reference to hematopoiesis. Methods: Two expressed sequence tags with translated sequence homologous to human NUP98 were identified. The gene was cloned by PCR from cDNA of zebrafish embryos. Expression of nup98 in zebrafish embryos was investigated spatially by whole-mount in-situ hybridization and temporally by RT-PCR. The functions of nup98 were examined by morpholino knockdown and the effects on embryonic development evaluated by gene expression studies and confocal microscopy. Cellular functions of zebrafish nup98 were investigated in HeLa cells. Results: Zebrafish nup98 gene shared 65% identity to human NUP98 homolog in protein sequence. The gene was expressed during early embryonic development since 1-cell stage and diffusely in eyes and the developing brain since 18 hpf. About 30% nup98-knockdown embryos developed intracranial hemorrhage at 48 hpf, resulting from disrupted blood vessels. nup98-knockdown upregulated pu.1 and scl as evaluated by quantitative RT-PCR. Moreover, ectopic expression of zebrafish nup98 rescued the defective mRNA export due to NUP98 knockdown in HeLa cells. Conclusion: A novel zebrafish nup98 gene was shown to exhibit conserved function in mRNA trafficking. Its role in embryonic development should be further evaluated. 26-
dc.languageengen_HK
dc.publisherThe University of Hong Kong.-
dc.relation.ispartof16th Hong Kong International Cancer Congresss and 6th Annual Meeting Centre for Cancer Research-
dc.titleFunctional characterization of a novel nucleoporin gene nup98 in zebrafish embryosen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailFung, TK: fungkan@gmail.comen_HK
dc.identifier.emailLeung, AYH: ayhleung@hku.hken_HK
dc.identifier.hkuros161881en_HK

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