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Conference Paper: New function of Krit 1 and as a new interactor of XAF1 in human colon cancers

TitleNew function of Krit 1 and as a new interactor of XAF1 in human colon cancers
Authors
Issue Date2007
PublisherJohn Wiley & Sons, Inc.
Citation
Asian Pacific Digestive Week, Kobe, Japan, 15-18 October 2007. In Journal of Gastroenterology and Hepatology, 2007, v. 22 n. S2, p. A162 Abstract no.TueP-058 How to Cite?
AbstractBackground: Suggested that XIAP-associated factor 1 (XAF1) as a potential tumor suppressor as it has recently been shown to negatively regulate the caspase-inhibiting activity of XIAP(X-linked IAP). The Krev interaction trapped-1(Krit1) has been demonstrated mutated in Cerebral Cavernous Malformation families. And the gene located at 7q21-q22, which the region is frequently deleted or amplifi ed in multiple forms of cancer. Objective: Identifi ed the interaction of XAF1 and Krit1,and investigate the novel function of Krit1 in human cancers. Methods: (1) A yeast two-hybrid screen was used to identify the activity of XAF1. (2) Microscopy and coimmunoprecipitation was used to detect the location of Krit1 and confi rmed the interaction of Krit1 and XAF1. (3) RT-PCR was used to investigated the expression of Kirt1 in human colon cancer cells and transient transfection and MTT were used to study Krit1 functions. Results: The Krit1 interacted with XAF1 and colocalized with membrane structures. Krit1 expressed in 7 of 8 human colon cancer cell lines. Overexpression of Krit1 increased 25% of cell death compared with untreated SW1116 cells. Multinucleus cells were induced after 48h combination treatment of Krit1 and XAF1. Conclusions: Krit1 is a novel interactor of XAF1. Krit1 may help determine cancer cell shape and function in response to cell death by guiding cytoskeletal structure and cell cycle.
Persistent Identifierhttp://hdl.handle.net/10722/102552
ISSN
2015 Impact Factor: 3.322
2015 SCImago Journal Rankings: 1.190

 

DC FieldValueLanguage
dc.contributor.authorZou, Ben_HK
dc.contributor.authorYang, Yen_HK
dc.contributor.authorZeng, Hen_HK
dc.contributor.authorWong, LWen_HK
dc.contributor.authorJiang, Sen_HK
dc.contributor.authorWong, BCYen_HK
dc.date.accessioned2010-09-25T20:35:15Z-
dc.date.available2010-09-25T20:35:15Z-
dc.date.issued2007en_HK
dc.identifier.citationAsian Pacific Digestive Week, Kobe, Japan, 15-18 October 2007. In Journal of Gastroenterology and Hepatology, 2007, v. 22 n. S2, p. A162 Abstract no.TueP-058-
dc.identifier.issn0815-9319-
dc.identifier.urihttp://hdl.handle.net/10722/102552-
dc.description.abstractBackground: Suggested that XIAP-associated factor 1 (XAF1) as a potential tumor suppressor as it has recently been shown to negatively regulate the caspase-inhibiting activity of XIAP(X-linked IAP). The Krev interaction trapped-1(Krit1) has been demonstrated mutated in Cerebral Cavernous Malformation families. And the gene located at 7q21-q22, which the region is frequently deleted or amplifi ed in multiple forms of cancer. Objective: Identifi ed the interaction of XAF1 and Krit1,and investigate the novel function of Krit1 in human cancers. Methods: (1) A yeast two-hybrid screen was used to identify the activity of XAF1. (2) Microscopy and coimmunoprecipitation was used to detect the location of Krit1 and confi rmed the interaction of Krit1 and XAF1. (3) RT-PCR was used to investigated the expression of Kirt1 in human colon cancer cells and transient transfection and MTT were used to study Krit1 functions. Results: The Krit1 interacted with XAF1 and colocalized with membrane structures. Krit1 expressed in 7 of 8 human colon cancer cell lines. Overexpression of Krit1 increased 25% of cell death compared with untreated SW1116 cells. Multinucleus cells were induced after 48h combination treatment of Krit1 and XAF1. Conclusions: Krit1 is a novel interactor of XAF1. Krit1 may help determine cancer cell shape and function in response to cell death by guiding cytoskeletal structure and cell cycle.-
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.-
dc.relation.ispartofJournal of Gastroenterology and Hepatologyen_HK
dc.titleNew function of Krit 1 and as a new interactor of XAF1 in human colon cancersen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailZou, B: zoubing@hkucc.hku.hken_HK
dc.identifier.emailWong, BCY: bcywong@hku.hken_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1746.2007.05192.x-
dc.identifier.hkuros131599en_HK

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