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Conference Paper: Aquaporin-4 expression by thymoma of myasthenia gravis patients

TitleAquaporin-4 expression by thymoma of myasthenia gravis patients
Authors
Issue Date2010
PublisherEuropean Neurological Society. The Journal's web site is located at http://www.link.springer.de/link/service/journals/00415/index.htm
Citation
The 12th Meeting of the European Neurological Society (ENS 2010), Berlin, Germany, 19-23 June 2010. In Journal of Neurology, 2010, v. 257 suppl. 1, p. S50, abstract no. O156 How to Cite?
AbstractBACKGROUND: Neuromyelitis optica (NMO) is a serious idiopathic inflammatory demyelinating disorder characterized by acute transverse myelitis and optic neuritis. A significant proportion of NMO patients are seropositive for NMO-IgG which is autoantibody against aquaporin-4 (AQP4) water channel. Paraneoplastic NMO associated various tumors were recently reported. Aim: We studied the expression of AQP4 by thymoma from patients with and without myasthenia gravis (MG). METHODS: Thymoma obtained from thymomectomy in patients with and without MG were studied by immunohistochemistry and western blot. RESULTS: Ten thymoma patients (9 with MG) and two control patients without thymoma or MG were studied. Immunohistochemistry revealed AQP4 immunoreactivity in cell membrane of thymoma cells from all ten thymoma specimens whereas thymic tissues from patients without thymoma or MG were negative for AQP4 immunoreactivity. Western blot revealed that lysates of nine of the ten thymoma specimens reacted with anti-human AQP4 antibody with a band of *30 kDa compatible with the molecular weight of AQP4. Interestingly, immunofluorescence revealed that IgG isolated from 2 NMO patients seropositive for NMO-IgG bound to cell membrane of thymoma cells from all ten thymoma specimens while IgG from healthy control subject did not. CONCLUSION: Thymoma cells of patients with and without MG express AQP4. NMO-IgG from serum of NMO patients bound to AQP4 expressed on thymoma cell membrane.
DescriptionOral session 20 - Neuro-oncology: abstract O156
Persistent Identifierhttp://hdl.handle.net/10722/102442

 

DC FieldValueLanguage
dc.contributor.authorChan, KHen_HK
dc.contributor.authorKwan, JSen_HK
dc.contributor.authorChu, ACYen_HK
dc.contributor.authorHo, WLen_HK
dc.contributor.authorHo, WMen_HK
dc.contributor.authorHo, SLen_HK
dc.date.accessioned2010-09-25T20:30:49Z-
dc.date.available2010-09-25T20:30:49Z-
dc.date.issued2010en_HK
dc.identifier.citationThe 12th Meeting of the European Neurological Society (ENS 2010), Berlin, Germany, 19-23 June 2010. In Journal of Neurology, 2010, v. 257 suppl. 1, p. S50, abstract no. O156-
dc.identifier.urihttp://hdl.handle.net/10722/102442-
dc.descriptionOral session 20 - Neuro-oncology: abstract O156-
dc.description.abstractBACKGROUND: Neuromyelitis optica (NMO) is a serious idiopathic inflammatory demyelinating disorder characterized by acute transverse myelitis and optic neuritis. A significant proportion of NMO patients are seropositive for NMO-IgG which is autoantibody against aquaporin-4 (AQP4) water channel. Paraneoplastic NMO associated various tumors were recently reported. Aim: We studied the expression of AQP4 by thymoma from patients with and without myasthenia gravis (MG). METHODS: Thymoma obtained from thymomectomy in patients with and without MG were studied by immunohistochemistry and western blot. RESULTS: Ten thymoma patients (9 with MG) and two control patients without thymoma or MG were studied. Immunohistochemistry revealed AQP4 immunoreactivity in cell membrane of thymoma cells from all ten thymoma specimens whereas thymic tissues from patients without thymoma or MG were negative for AQP4 immunoreactivity. Western blot revealed that lysates of nine of the ten thymoma specimens reacted with anti-human AQP4 antibody with a band of *30 kDa compatible with the molecular weight of AQP4. Interestingly, immunofluorescence revealed that IgG isolated from 2 NMO patients seropositive for NMO-IgG bound to cell membrane of thymoma cells from all ten thymoma specimens while IgG from healthy control subject did not. CONCLUSION: Thymoma cells of patients with and without MG express AQP4. NMO-IgG from serum of NMO patients bound to AQP4 expressed on thymoma cell membrane.-
dc.languageengen_HK
dc.publisherEuropean Neurological Society. The Journal's web site is located at http://www.link.springer.de/link/service/journals/00415/index.htm-
dc.relation.ispartofJournal of Neurology-
dc.titleAquaporin-4 expression by thymoma of myasthenia gravis patientsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailChan, KH: koonho@hkucc.hku.hken_HK
dc.identifier.emailKwan, JS: jsckwan@HKUCC.hku.hken_HK
dc.identifier.emailChu, ACY: bcccy@hkucc.hku.hken_HK
dc.identifier.emailHo, WL: hwl2002@hkusua.hku.hken_HK
dc.identifier.emailHo, WM: hello_seeka@yahoo.com.hken_HK
dc.identifier.emailHo, SL: slho@hku.hken_HK
dc.identifier.authorityChan, KH=rp00537en_HK
dc.identifier.authorityChu, ACY=rp00505en_HK
dc.identifier.doi10.1007/s00415-010-5575-7-
dc.identifier.hkuros169498en_HK
dc.identifier.volume257-
dc.identifier.issuesuppl. 1-
dc.identifier.spageS50, abstract no. O156-
dc.identifier.epageS50, abstract no. O156-
dc.publisher.placeSwitzerland-

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