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Article: Detection of T-cell receptor delta gene rearrangement by clonal specific polymerase chain reaction

TitleDetection of T-cell receptor delta gene rearrangement by clonal specific polymerase chain reaction
Authors
Issue Date1997
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leu
Citation
Leukemia, 1997, v. 11 suppl. 3, p. 281-284 How to Cite?
AbstractA sensitive and specific technique to detect minimal residual disease for T-cell malignancies was explored. Southern analysis and polymerase chain reaction (PCR) were used to detect the rearranged V-D-J segment of T-cell receptor delta (TCR delta) gene from malignant cell specimens of patients with leukemia and lymphoma of T-cell lineage. The PCR product was sequenced and from the DNA sequences of the V-D-J region, a 3' antisense primer was designed and synthesized for clonal specific PCR (CS-PCR). Seven of the 22 T-ALL (32%) and 5 of 18 (28%) T-cell lymphoma showed clonal rearrangement by Southern analysis. Six of the 7 (86%) T-ALL and 4 of the 5 (80%) T-cell lymphoma which were Southern positive were also positive by TCR delta PCR. The PCR products of four cases of T-ALL showing clonal pattern by TCR delta PCR amplification were successfully sequenced and CS-PCR amplification performed. CS-PCR detected with confidence specific clonal rearrangement in a mixture containing 0.003% of malignant cells. Marrow specimens obtained at diagnosis and subsequent follow-ups from the 4 T-ALL patients were studied by Southern analysis, TCR delta PCR and CS-PCR. The first patient was in continuous morphological complete remission for more than 3 years and had persistently negative Southern, TCR delta PCR and CS-PCR results on follow-up. Initial follow-up marrow samples from the second patient had persistently positive CS-PCR results while they were still morphologically and TCR delta PCR negative and the patient had a frank leukemic relapse at 18 months. The other two patients had persistent disease by conventional morphological examination, Southern analysis, TCR delta PCR and CS-PCR studies were all positive as expected. CS-PCR is a highly specific and sensitive technique in detecting minimal residual disease for T-cell malignancies. Its potential applications warrant further clinical evaluation and correlation.
Persistent Identifierhttp://hdl.handle.net/10722/102261
ISSN
2015 Impact Factor: 12.104
2015 SCImago Journal Rankings: 5.142

 

DC FieldValueLanguage
dc.contributor.authorChan, DW-
dc.contributor.authorLiang, RHS-
dc.contributor.authorChan, VNY-
dc.contributor.authorKwong, YL-
dc.contributor.authorChan, TK-
dc.date.accessioned2010-09-25T20:23:31Z-
dc.date.available2010-09-25T20:23:31Z-
dc.date.issued1997-
dc.identifier.citationLeukemia, 1997, v. 11 suppl. 3, p. 281-284-
dc.identifier.issn0887-6924-
dc.identifier.urihttp://hdl.handle.net/10722/102261-
dc.description.abstractA sensitive and specific technique to detect minimal residual disease for T-cell malignancies was explored. Southern analysis and polymerase chain reaction (PCR) were used to detect the rearranged V-D-J segment of T-cell receptor delta (TCR delta) gene from malignant cell specimens of patients with leukemia and lymphoma of T-cell lineage. The PCR product was sequenced and from the DNA sequences of the V-D-J region, a 3' antisense primer was designed and synthesized for clonal specific PCR (CS-PCR). Seven of the 22 T-ALL (32%) and 5 of 18 (28%) T-cell lymphoma showed clonal rearrangement by Southern analysis. Six of the 7 (86%) T-ALL and 4 of the 5 (80%) T-cell lymphoma which were Southern positive were also positive by TCR delta PCR. The PCR products of four cases of T-ALL showing clonal pattern by TCR delta PCR amplification were successfully sequenced and CS-PCR amplification performed. CS-PCR detected with confidence specific clonal rearrangement in a mixture containing 0.003% of malignant cells. Marrow specimens obtained at diagnosis and subsequent follow-ups from the 4 T-ALL patients were studied by Southern analysis, TCR delta PCR and CS-PCR. The first patient was in continuous morphological complete remission for more than 3 years and had persistently negative Southern, TCR delta PCR and CS-PCR results on follow-up. Initial follow-up marrow samples from the second patient had persistently positive CS-PCR results while they were still morphologically and TCR delta PCR negative and the patient had a frank leukemic relapse at 18 months. The other two patients had persistent disease by conventional morphological examination, Southern analysis, TCR delta PCR and CS-PCR studies were all positive as expected. CS-PCR is a highly specific and sensitive technique in detecting minimal residual disease for T-cell malignancies. Its potential applications warrant further clinical evaluation and correlation.-
dc.languageeng-
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leu-
dc.relation.ispartofLeukemia-
dc.subject.meshGene Rearrangement, delta-Chain T-Cell Antigen Receptor-
dc.subject.meshLeukemia, T-Cell - genetics - immunology - mortality - pathology-
dc.subject.meshLymphoma, T-Cell - genetics - immunology - mortality - pathology-
dc.subject.meshPolymerase Chain Reaction - methods-
dc.subject.meshBase Sequence-
dc.titleDetection of T-cell receptor delta gene rearrangement by clonal specific polymerase chain reaction-
dc.typeArticle-
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0887-6924&volume=11&issue=supplement 3&spage=281&epage=284&date=1997&atitle=Detection+of+T-cell+recptor+delta+gene+rearrangement+by+clonal+specific+polymerase+chain+reactionen_HK
dc.identifier.emailChan, DW: dwchan@hku.hk-
dc.identifier.emailLiang, RHS: rliang@hku.hk-
dc.identifier.emailChan, VNY: vnychana@hku.hk-
dc.identifier.emailKwong, YL: ylkwong@hku.hk-
dc.identifier.authorityChan, DW=rp00543-
dc.identifier.authorityLiang, RHS=rp00345-
dc.identifier.authorityChan, VNY=rp00320-
dc.identifier.authorityKwong, YL=rp00358-
dc.identifier.pmid9209365-
dc.identifier.hkuros28476-
dc.identifier.volume11-
dc.identifier.issuesuppl. 3-
dc.identifier.spage281-
dc.identifier.epage284-
dc.publisher.placeUnited Kingdom-

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