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Conference Paper: Hypermethylation status around exon 1 of XIAP-associated factor 1 (XAF1) in human gastric andcolon cancer cell lines

TitleHypermethylation status around exon 1 of XIAP-associated factor 1 (XAF1) in human gastric andcolon cancer cell lines
Authors
Issue Date2005
PublisherBlackwell Publishing Asia.
Citation
Asian Pacific Digestive Week, Seoul, Korea, 25-28 September 2005. In Journal of Gastroenterology and Hepatology, 2005, v. 20 n. S2, p. A370 Abstract no. WP143 How to Cite?
AbstractBackground and Aims XIAP-associated factor 1 (XAF1) hasrecently been shown to negatively regulate the caspase-inhibitingactivity of XIAP (X-linked IAP), thus, XAF1 may play a pivotal rolein cancer cell apoptosis. The XAF1 mRNA is expressed at low orundetectable levels in various cancer cell lines, suggesting that thegene silencing in cancer cells is important for malignant cell survival.The aim of this study is to examine the role of promoter and exon 1hypermethylation in the transcriptional silencing of XAF1. Methods (1) We treated the gastric cancer cell lines AGS, MKN-45, BCG823 and colon cancer cell lines SW1116, HCT 15, colo 205with 5¢-AZA-2¢-deoxycytidine (5¢-AZA), then the mRNA levels weredetected by RT-PCR. (2) Genomic DNAs were isolated from thesecell lines and human normal samples, the methylation status wasdetermined by bisulfite DNA sequencing analysis for 8 CpG siteslocated around exon 1 area (nucleotides -40 to 194).Results The XAF1 mRNA were detected in all six cell lines. Therelative mRNA level ranked HCT 15 < AGS < SW1116,BCG 823
Persistent Identifierhttp://hdl.handle.net/10722/101933
ISSN
2014 Impact Factor: 3.504
2014 SCImago Journal Rankings: 1.128

 

DC FieldValueLanguage
dc.contributor.authorZou, Ben_HK
dc.contributor.authorLin, MCen_HK
dc.contributor.authorLam, SKen_HK
dc.contributor.authorTu, Sen_HK
dc.contributor.authorZeng, Hen_HK
dc.contributor.authorYang, Yen_HK
dc.contributor.authorWang, Jen_HK
dc.contributor.authorHe, Hen_HK
dc.contributor.authorJiang, SHen_HK
dc.contributor.authorKung, Hen_HK
dc.contributor.authorWong, BCYen_HK
dc.date.accessioned2010-09-25T20:10:19Z-
dc.date.available2010-09-25T20:10:19Z-
dc.date.issued2005en_HK
dc.identifier.citationAsian Pacific Digestive Week, Seoul, Korea, 25-28 September 2005. In Journal of Gastroenterology and Hepatology, 2005, v. 20 n. S2, p. A370 Abstract no. WP143en_HK
dc.identifier.issn0815-9319en_HK
dc.identifier.urihttp://hdl.handle.net/10722/101933-
dc.description.abstractBackground and Aims XIAP-associated factor 1 (XAF1) hasrecently been shown to negatively regulate the caspase-inhibitingactivity of XIAP (X-linked IAP), thus, XAF1 may play a pivotal rolein cancer cell apoptosis. The XAF1 mRNA is expressed at low orundetectable levels in various cancer cell lines, suggesting that thegene silencing in cancer cells is important for malignant cell survival.The aim of this study is to examine the role of promoter and exon 1hypermethylation in the transcriptional silencing of XAF1. Methods (1) We treated the gastric cancer cell lines AGS, MKN-45, BCG823 and colon cancer cell lines SW1116, HCT 15, colo 205with 5¢-AZA-2¢-deoxycytidine (5¢-AZA), then the mRNA levels weredetected by RT-PCR. (2) Genomic DNAs were isolated from thesecell lines and human normal samples, the methylation status wasdetermined by bisulfite DNA sequencing analysis for 8 CpG siteslocated around exon 1 area (nucleotides -40 to 194).Results The XAF1 mRNA were detected in all six cell lines. Therelative mRNA level ranked HCT 15 < AGS < SW1116,BCG 823 <Colo 205 < MKN-45. 5¢-AZA treatment significantly upregulated thegene expression of XAF1 in all of the cancer cell lines with the excep-tion of Colo205. HCT 15 and AGS were most sentitive to 5¢-AZAtreatment suggesting that they have the highest methylation status. Bybisulfite DNA sequencing method,we showed that normal blood celldo not display XAF1 methylation. In contrast, all cancer cell linesshowed different levels of hypermethylation of exon 1 except Colo205. Among the 5 cell lines, HCT 15 and AGS displayed highest levelof hypermethylation.Conclusions Results from our study showed that the exon 1 hyper-methylation status of XAF1 coincidences with the gene expressionsuggesting that the aberrant exon 1 methylation contributes at leastin part to the epigenetic silencing of XAF1, which may be importantfor malignant cell survival.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia.en_HK
dc.relation.ispartofJournal of Gastroenterology and Hepatologyen_HK
dc.titleHypermethylation status around exon 1 of XIAP-associated factor 1 (XAF1) in human gastric andcolon cancer cell linesen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0815-9319&volume=20&spage=Supp A370&epage=&date=2005&atitle=Hypermethylation+status+around+exon+1+of+XIAP-Associated+Factor+1(XAF1)++in+human+gastric+and+colon+cancer+cell+line. Asian+Pacific+Digestive+Week,+Sept.+25-28,+2005,+Seoul,+Korea.en_HK
dc.identifier.emailZou, B: zoubing@hkucc.hku.hken_HK
dc.identifier.emailLin, MC: mcllin@HKUCC.hku.hken_HK
dc.identifier.emailLam, SK: deanmed@hku.hken_HK
dc.identifier.emailWang, J: jidewang@gmail.comen_HK
dc.identifier.emailHe, H: cecily_bb3@hotmail.comen_HK
dc.identifier.emailKung, H: hkung@hkucc.hku.hken_HK
dc.identifier.emailWong, BCY: bcywong@hku.hken_HK
dc.identifier.authorityLin, MC=rp00746en_HK
dc.identifier.authorityWang, J=rp00491en_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1746.2005.04102.x-
dc.identifier.hkuros117563en_HK
dc.identifier.volume20en_HK

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