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Conference Paper: Characterization of calcium signaling pathways in human cardiac fibroblast

TitleCharacterization of calcium signaling pathways in human cardiac fibroblast
Authors
Issue Date2008
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at https://febs.onlinelibrary.wiley.com/loi/17424658/year/2008
Citation
The 33rd FEBS Congress, Athens, Greece, 28 June-3 July 2008. In FEBS Journal, 2008, v. 275 n. S1, p. 350 How to Cite?
AbstractIntroduction: Ca2+ signals play an important role in the physiological activities of many types of cells. Though cardiac fibroblasts play an essential role in the maintenance of extracellular matrix in the normal heart and as mediators of inflammatory and fibrotic myocardial remodelling in the injured and failing heart, Ca2+ signaling pathways in human cardiac fibroblast (HCF) are poorly understood. The present study aims to characterize Ca2+ signaling pathways in HCFs. Methods: Intracellular Ca2+ activity was determined using a confocal microscopy and gene expression was examined with RT-PCR. Results: [Ca2+]i oscillations were present in 30% of cells and were dependent on Ca2+ entry. The oscillatory frequency was reduced by the voltage-operated Ca2+ channel (VOCC) blocker Nifedipine and Ca2+ oscillations were abolished by the store-operated Ca2+ entry (SOCE) channel blocker La3+. The PLC inhibitor U-73122 and the InsP3R inhibitor 2-APB, but not RyR blocker ryanodine, abolished the Ca2+ oscillations, while InsP3R agonist thimerosal enhanced Ca2+ oscillations. RT-PCR revealed molecular identities of Ca2+ signal pathways, including mRNAs for InsP3R1-3, SERCA1-3, L-type Ca2+ channels and the store-operated Ca2+ channel-related genes including TRPC1, 3, 4, 6 were significant, but not mRNAs for ryanodine receptor in HCFs. Conclusion: Our results firstly demonstrate that spontaneous Ca2+ oscillations are present in cultured HCFs, and [Ca2+]i mobilization in HCF is likely mediated by both SOCE and VOCCs and mobilized by InsP3 via PLC activation. Mechanisms of the spontaneous Ca2+ oscillations and role of them in cellular physiology remain to be studied in HCF.
Persistent Identifierhttp://hdl.handle.net/10722/101691
ISSN
2023 Impact Factor: 5.5

 

DC FieldValueLanguage
dc.contributor.authorChen, Jen_HK
dc.contributor.authorLau, CPen_HK
dc.contributor.authorLi, GRen_HK
dc.date.accessioned2010-09-25T19:59:55Z-
dc.date.available2010-09-25T19:59:55Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 33rd FEBS Congress, Athens, Greece, 28 June-3 July 2008. In FEBS Journal, 2008, v. 275 n. S1, p. 350en_HK
dc.identifier.issn1742-4658-
dc.identifier.urihttp://hdl.handle.net/10722/101691-
dc.description.abstractIntroduction: Ca2+ signals play an important role in the physiological activities of many types of cells. Though cardiac fibroblasts play an essential role in the maintenance of extracellular matrix in the normal heart and as mediators of inflammatory and fibrotic myocardial remodelling in the injured and failing heart, Ca2+ signaling pathways in human cardiac fibroblast (HCF) are poorly understood. The present study aims to characterize Ca2+ signaling pathways in HCFs. Methods: Intracellular Ca2+ activity was determined using a confocal microscopy and gene expression was examined with RT-PCR. Results: [Ca2+]i oscillations were present in 30% of cells and were dependent on Ca2+ entry. The oscillatory frequency was reduced by the voltage-operated Ca2+ channel (VOCC) blocker Nifedipine and Ca2+ oscillations were abolished by the store-operated Ca2+ entry (SOCE) channel blocker La3+. The PLC inhibitor U-73122 and the InsP3R inhibitor 2-APB, but not RyR blocker ryanodine, abolished the Ca2+ oscillations, while InsP3R agonist thimerosal enhanced Ca2+ oscillations. RT-PCR revealed molecular identities of Ca2+ signal pathways, including mRNAs for InsP3R1-3, SERCA1-3, L-type Ca2+ channels and the store-operated Ca2+ channel-related genes including TRPC1, 3, 4, 6 were significant, but not mRNAs for ryanodine receptor in HCFs. Conclusion: Our results firstly demonstrate that spontaneous Ca2+ oscillations are present in cultured HCFs, and [Ca2+]i mobilization in HCF is likely mediated by both SOCE and VOCCs and mobilized by InsP3 via PLC activation. Mechanisms of the spontaneous Ca2+ oscillations and role of them in cellular physiology remain to be studied in HCF.-
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at https://febs.onlinelibrary.wiley.com/loi/17424658/year/2008-
dc.relation.ispartofFEBS Journalen_HK
dc.titleCharacterization of calcium signaling pathways in human cardiac fibroblasten_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLau, CP: cplau@hku.hken_HK
dc.identifier.emailLi, GR: grli@hkucc.hku.hken_HK
dc.identifier.authorityLi, GR=rp00476en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.hkuros152953en_HK
dc.identifier.volume275en_HK
dc.identifier.issuesuppl 1en_HK
dc.identifier.spage350en_HK
dc.identifier.epage350-
dc.identifier.issnl1742-464X-

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