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Conference Paper: Mice overexpressing amyloid precursor protein (APP) and harbouring presenilin-1 (PS1) gene are more susceptible to photothrombotic stroke and post-stroke memory impairment than APP transgenic mice

TitleMice overexpressing amyloid precursor protein (APP) and harbouring presenilin-1 (PS1) gene are more susceptible to photothrombotic stroke and post-stroke memory impairment than APP transgenic mice
Authors
KeywordsSENSORIMOTOR
MEMORY TEST
INFARCT
ROSE BENGAL
Issue Date2003
PublisherSociety for Neuroscience
Citation
Neuroscience 2003, New Orleans, LA, 8-12 November 2003, Presentation no. 114.4 How to Cite?
AbstractWe used Rose Bengal (RB; 1 mg IV at 1 min before illumination for 15 min) to induce photothrombotic stroke in 3-month-old mice overexpressing human APP with or without human mutant PS1 gene. Sham stroke groups received RB without illumination. Regional cerebral blood flow (CBF) over the penumbra was monitored during illumination in 2 groups of mice. Two days after real or sham stroke, sensorimotor functions were assessed prior to determination of infarct volume (IFV) in some groups using triphenyltetrazolium chloride staining. Seven days after real or sham stroke, sensorimotor functions and memory tests were done prior to IFV assessment in other groups. One group of mice received a longer illumination of 20 min and was sacrificed 7 days later. Reduction in CBF during illumination was comparable between the APP and APP/PS1 mice. Two days after stroke, the relative IFV was, in mean±SEM, 6.59±0.87% (n=8) and 8.19±0.82% (n=8) in the APP and APP/PS1 mice, respectively. Compared with the sham stroke group, a significant increase in the time required to turn around inside an alley of similar extent was seen in the transgenic mice at 2 days after stroke; there was no difference in the latency of falling from a pole. Seven days after stroke, the relative infarct volume was similar in both groups of APP mice undergone 15 or 20 min of illumination (5.85±0.94%; n=12), but this was smaller than that of the APP/PS1 transgenic mice (9.15±0.91%; n=6; P<0.01). When compared to the sham stroke groups, there was no difference in the sensorimotor functions, and time spent in the target quadrant of the Morris water maze test was reduced in the APP/PS1 (P<0.05) but not APP transgenic mice. In conclusion, the APP/PS1 double transgenic mice are more susceptible to photothrombotic stroke than the APP transgenic mice with a larger IFV and manifestation of memory impairment.
Persistent Identifierhttp://hdl.handle.net/10722/101604

 

DC FieldValueLanguage
dc.contributor.authorZou, Len_HK
dc.contributor.authorTang, Fen_HK
dc.contributor.authorCheung, RTFen_HK
dc.date.accessioned2010-09-25T19:56:25Z-
dc.date.available2010-09-25T19:56:25Z-
dc.date.issued2003en_HK
dc.identifier.citationNeuroscience 2003, New Orleans, LA, 8-12 November 2003, Presentation no. 114.4-
dc.identifier.urihttp://hdl.handle.net/10722/101604-
dc.description.abstractWe used Rose Bengal (RB; 1 mg IV at 1 min before illumination for 15 min) to induce photothrombotic stroke in 3-month-old mice overexpressing human APP with or without human mutant PS1 gene. Sham stroke groups received RB without illumination. Regional cerebral blood flow (CBF) over the penumbra was monitored during illumination in 2 groups of mice. Two days after real or sham stroke, sensorimotor functions were assessed prior to determination of infarct volume (IFV) in some groups using triphenyltetrazolium chloride staining. Seven days after real or sham stroke, sensorimotor functions and memory tests were done prior to IFV assessment in other groups. One group of mice received a longer illumination of 20 min and was sacrificed 7 days later. Reduction in CBF during illumination was comparable between the APP and APP/PS1 mice. Two days after stroke, the relative IFV was, in mean±SEM, 6.59±0.87% (n=8) and 8.19±0.82% (n=8) in the APP and APP/PS1 mice, respectively. Compared with the sham stroke group, a significant increase in the time required to turn around inside an alley of similar extent was seen in the transgenic mice at 2 days after stroke; there was no difference in the latency of falling from a pole. Seven days after stroke, the relative infarct volume was similar in both groups of APP mice undergone 15 or 20 min of illumination (5.85±0.94%; n=12), but this was smaller than that of the APP/PS1 transgenic mice (9.15±0.91%; n=6; P<0.01). When compared to the sham stroke groups, there was no difference in the sensorimotor functions, and time spent in the target quadrant of the Morris water maze test was reduced in the APP/PS1 (P<0.05) but not APP transgenic mice. In conclusion, the APP/PS1 double transgenic mice are more susceptible to photothrombotic stroke than the APP transgenic mice with a larger IFV and manifestation of memory impairment.-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meetingen_HK
dc.subjectSENSORIMOTOR-
dc.subjectMEMORY TEST-
dc.subjectINFARCT-
dc.subjectROSE BENGAL-
dc.titleMice overexpressing amyloid precursor protein (APP) and harbouring presenilin-1 (PS1) gene are more susceptible to photothrombotic stroke and post-stroke memory impairment than APP transgenic miceen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.emailCheung, RTF: rtcheung@hku.hken_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.identifier.authorityCheung, RTF=rp00434en_HK
dc.identifier.hkuros87615en_HK

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