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Conference Paper: Effect of insulin on endothelial lipase in type 2 diabetes

TitleEffect of insulin on endothelial lipase in type 2 diabetes
Authors
Issue Date2008
PublisherElsevier Science Pub. Co.
Citation
The 7th International Diabetes Federation Western Pacific Region Congress, Wellington, New Zealand, 31 March-3 April 2008. In Diabetes Research and Clinical Practice, 2008, v. 79 n. S1, p. S69 Abstract no. P-38 How to Cite?
AbstractObjective: Endothelial lipase (EL), a new member of the triacylglycerol lipase family, modulates the metabolism of high-density lipoproteins and is upregulated by inflammatory cytokines. Since Type 2 diabetes is associated with chronic subclinical inflammation, we have determined whether serum EL concentration is increased in patients with Type 2 diabetes and investigated the effect of insulin on EL. Methods: A total of 237 patients with Type 2 diabetes on oral anti-diabetic agents, 111 patients with Type 2 diabetes on insulin therapy and 226 non-diabetic controls were recruited. Serum EL was measured by ELISA. To investigate the effect of insulin on EL production by endothelial cells, human aortic endothelial cells were incubated with insulin and EL mRNA and protein in the cell medium was measured. Serum EL was also measured in 16 diabetic subjects before and after starting insulin therapy. Results: Serum EL levels were highest in patients on oral antidiabetic agents, whereas those on insulin had similar EL levels as controls (oral: 26.7 ± 16.1 ng/ml; insulin: 23.3 ± 11.6, controls: 23.9 ± 12.0; ANOVA p=0.04). In both controls and patients on oral anti-diabetic agents, EL correlated with log(CRP) (r=0.20, p=0.003; r=0.23, p<0.001 respectively), but no correlation was seen in patients on insulin. In vitro experiments showed that insulin significantly reduced EL mRNA and protein in human aortic endothelial cells in a dose-dependent manner. Serum EL concentration also decreased significantly in diabetic patients after starting insulin therapy (p<0.03). Conclusion: Serum EL concentration was increased in patients with Type 2 diabetes and was associated with the degree of subclinical inflammation. Exogenous insulin therapy lowered serum EL concentration.
Persistent Identifierhttp://hdl.handle.net/10722/101511
ISSN
2015 Impact Factor: 3.045
2015 SCImago Journal Rankings: 1.338

 

DC FieldValueLanguage
dc.contributor.authorTan, KCBen_HK
dc.contributor.authorShiu, SWMen_HK
dc.contributor.authorHuang, Yen_HK
dc.contributor.authorWong, Yen_HK
dc.date.accessioned2010-09-25T19:52:38Z-
dc.date.available2010-09-25T19:52:38Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 7th International Diabetes Federation Western Pacific Region Congress, Wellington, New Zealand, 31 March-3 April 2008. In Diabetes Research and Clinical Practice, 2008, v. 79 n. S1, p. S69 Abstract no. P-38-
dc.identifier.issn0168-8227-
dc.identifier.urihttp://hdl.handle.net/10722/101511-
dc.description.abstractObjective: Endothelial lipase (EL), a new member of the triacylglycerol lipase family, modulates the metabolism of high-density lipoproteins and is upregulated by inflammatory cytokines. Since Type 2 diabetes is associated with chronic subclinical inflammation, we have determined whether serum EL concentration is increased in patients with Type 2 diabetes and investigated the effect of insulin on EL. Methods: A total of 237 patients with Type 2 diabetes on oral anti-diabetic agents, 111 patients with Type 2 diabetes on insulin therapy and 226 non-diabetic controls were recruited. Serum EL was measured by ELISA. To investigate the effect of insulin on EL production by endothelial cells, human aortic endothelial cells were incubated with insulin and EL mRNA and protein in the cell medium was measured. Serum EL was also measured in 16 diabetic subjects before and after starting insulin therapy. Results: Serum EL levels were highest in patients on oral antidiabetic agents, whereas those on insulin had similar EL levels as controls (oral: 26.7 ± 16.1 ng/ml; insulin: 23.3 ± 11.6, controls: 23.9 ± 12.0; ANOVA p=0.04). In both controls and patients on oral anti-diabetic agents, EL correlated with log(CRP) (r=0.20, p=0.003; r=0.23, p<0.001 respectively), but no correlation was seen in patients on insulin. In vitro experiments showed that insulin significantly reduced EL mRNA and protein in human aortic endothelial cells in a dose-dependent manner. Serum EL concentration also decreased significantly in diabetic patients after starting insulin therapy (p<0.03). Conclusion: Serum EL concentration was increased in patients with Type 2 diabetes and was associated with the degree of subclinical inflammation. Exogenous insulin therapy lowered serum EL concentration.-
dc.languageengen_HK
dc.publisherElsevier Science Pub. Co.-
dc.relation.ispartofDiabetes Research and Clinical Practiceen_HK
dc.titleEffect of insulin on endothelial lipase in type 2 diabetesen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTan, KCB: kcbtan@hku.hken_HK
dc.identifier.emailShiu, SWM: swmshiu@HKUSUA.hku.hken_HK
dc.identifier.emailWong, Y: ywong@HKUCC-COM.hku.hken_HK
dc.identifier.authorityTan, KCB=rp00402en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0168-8227(08)70812-X-
dc.identifier.hkuros145710en_HK

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