File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Conference Paper: Cyclooxygenase-1 and -2 gene disruption potentiateTH1 polarization in response to helicobacter pylori

TitleCyclooxygenase-1 and -2 gene disruption potentiateTH1 polarization in response to helicobacter pylori
Authors
Issue Date2004
PublisherBlackwell Publishing Asia.
Citation
Asian-Pacific Digestive Week 2004, Beijing, China, 4-7 October 2004. In Journal of Gastroenterology and Hepatology, 2004, v. 19 n. S5, p. A366 Abstract no. PO-148 How to Cite?
AbstractObjectives We have demonstrated that COX-1 and COX-2 dis-ruption enhances H. pylori (Hp)-induced gastritis. This study aimedto determine whether this effect is through the potentiation of theTh1 response and/or compensatory production of leukotrienes (LTs).Methods Hp strain TN2 was inoculated into the stomachs of COX-1 and COX-2 deficient homozygous (COX-1-/-, COX-2-/-) and con-geneic wild-type (WT) mice. Mice were sacrificed 24 wks later (n =8–10 mice/group). WT, COX-1-/- and COX-2-/- mice without Hpinoculation were used as controls. The expression of gastric mucosalIL-12, IFN-g, IL-10 and IL-4 mRNA was determined by RT-PCR,and the levels of LTB4and LTC4proteins were detected by ELISA. Results In the presence of Hp infection, expression of IL-12, IFN-g, IL-10 and IL-4 mRNA was elevated in WT mice, but only IL-12and IFN-g mRNA expression were further increased in both COX-1-/- and COX-2-/- mice. Both LTB4and LTC4levels were increasedin Hp infected WT, COX-1-/- and COX-2-/- mice compared withtheir uninfected controls, but there was no further increase in LTB4and LTC4production in both COX-1-/- and COX-2-/- mice (table).Conclusion COX-1 and COX-2 disruption enhances Hp-inducedgastritis is associated with the potentiation of Th1 polarization, butnot with compensatory LT production.
Persistent Identifierhttp://hdl.handle.net/10722/101293
ISSN
2015 Impact Factor: 3.322
2015 SCImago Journal Rankings: 1.190

 

DC FieldValueLanguage
dc.contributor.authorXia, HHXen_HK
dc.contributor.authorLi, Gen_HK
dc.contributor.authorChen, MHen_HK
dc.contributor.authorChan, OOen_HK
dc.contributor.authorCho, CHen_HK
dc.contributor.authorLam, SKen_HK
dc.contributor.authorBerg, Den_HK
dc.contributor.authorFeng, Zen_HK
dc.contributor.authorLangenbach, Ren_HK
dc.contributor.authorWong, BCYen_HK
dc.date.accessioned2010-09-25T19:43:45Z-
dc.date.available2010-09-25T19:43:45Z-
dc.date.issued2004en_HK
dc.identifier.citationAsian-Pacific Digestive Week 2004, Beijing, China, 4-7 October 2004. In Journal of Gastroenterology and Hepatology, 2004, v. 19 n. S5, p. A366 Abstract no. PO-148en_HK
dc.identifier.issn0815-9319en_HK
dc.identifier.urihttp://hdl.handle.net/10722/101293-
dc.description.abstractObjectives We have demonstrated that COX-1 and COX-2 dis-ruption enhances H. pylori (Hp)-induced gastritis. This study aimedto determine whether this effect is through the potentiation of theTh1 response and/or compensatory production of leukotrienes (LTs).Methods Hp strain TN2 was inoculated into the stomachs of COX-1 and COX-2 deficient homozygous (COX-1-/-, COX-2-/-) and con-geneic wild-type (WT) mice. Mice were sacrificed 24 wks later (n =8–10 mice/group). WT, COX-1-/- and COX-2-/- mice without Hpinoculation were used as controls. The expression of gastric mucosalIL-12, IFN-g, IL-10 and IL-4 mRNA was determined by RT-PCR,and the levels of LTB4and LTC4proteins were detected by ELISA. Results In the presence of Hp infection, expression of IL-12, IFN-g, IL-10 and IL-4 mRNA was elevated in WT mice, but only IL-12and IFN-g mRNA expression were further increased in both COX-1-/- and COX-2-/- mice. Both LTB4and LTC4levels were increasedin Hp infected WT, COX-1-/- and COX-2-/- mice compared withtheir uninfected controls, but there was no further increase in LTB4and LTC4production in both COX-1-/- and COX-2-/- mice (table).Conclusion COX-1 and COX-2 disruption enhances Hp-inducedgastritis is associated with the potentiation of Th1 polarization, butnot with compensatory LT production.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia.en_HK
dc.relation.ispartofJournal of Gastroenterology and Hepatologyen_HK
dc.titleCyclooxygenase-1 and -2 gene disruption potentiateTH1 polarization in response to helicobacter pylorien_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0815-9319&volume=19&issue=Suppl.&spage=A366&epage=&date=2004&atitle=Cyclooxygenase-1+and+-2+Gene+Disruption+Potentiate+TH1+Polarization+in+Response+to+Helicobacter+pylorien_HK
dc.identifier.emailXia, HHX: xiaharry@hotmail.comen_HK
dc.identifier.emailChan, OO: aoochan@hku.hken_HK
dc.identifier.emailCho, CH: chcho@hkusua.hku.hken_HK
dc.identifier.emailLam, SK: deanmed@hku.hken_HK
dc.identifier.emailFeng, Z: zhfeng@hku.hken_HK
dc.identifier.emailWong, BCY: bcywong@hku.hken_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1440-1746.2004.03623.x-
dc.identifier.hkuros96323en_HK
dc.identifier.volume19en_HK
dc.identifier.issueS5en_HK
dc.identifier.spage366en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats