Dr Wong, Yee Man Elaine 王怡雯
Dr Elaine Wong joined the Department of Biochemistry as a Research Assistant Professor in February 2013. At HKU, Dr. Wong obtained her PhD degree in 2006 and completed her post-doctoral training in developmental biology and molecular genetics. Dr. Wong underwent her second post-doctoral training in inner ear developmental and regenerative biology in Mount Sinai School of Medicine in New York in 2010. She is interested in understanding regulatory networks controlling mammalian auditory system development and complex disease mechanisms underlying human deafness, craniofacial and hindbrain disorders.
My research interest has been on the understanding of regulatory networks controlling mammalian organogenesis. More than 10 years’ research experience in developmental biology and hearing research. Disease mechanisms underlying human deafness and craniofacial disorders using knockout, transgenic mice, cellular and molecular approaches. Sonic hedgehog signaling genes in inner ear development; Eya1, Six1 and Hox genes regulatory networks associated to Human Branchio-oto-renal syndrome.
Major research themes and current projects
To understand the regulatory networks controlling mammalian organogenesis and complex disease mechanisms underlying human deafness. (1) Analyses of abnormal inner ear development of Irx3/5 knockout mutants to understand the mechanisms of Human Hamamy syndrome; (2) Genetic analyses of Cdo and Boc function in inner ear development; (3) The role of ciliogenesis in progressive hearing loss; (4) Regeneration of hair cells for treatment of human deafness by Human ES cells.
Techniques involve mammalian cochlear cell culture and real-time PCR, next generation sequencing (RNAseq), gene editing, ChIP sequencing and bioinformatics analyses. More than 10 years inner ear research experiences with auditory electrophysiology, hair cell functional analysis Patch clamp, isolation of hair cells, ips and ES cell culture, paint-filling for inner ear morphogenesis. Diverse cell-based assays such as viability and cell death, flow cytometry, Western Blotting, RNAi and coimmunoprecipitation CoIP experiments. Gene manipulation in mouse embryos and cochlea tissues techniques by injecting gene specific sh-RNA to generate loss-of-function system or overexpressing the target gene for gain-of-function system. Imaging techniques with multiphoton confocal live cell imaging. Neuronal dye tracing for brain and inner ear innervations, high resolution electron microscopy SEM, ultrastructural localization of the proteins involved in the stereocilia bundle at the top of sensory hair cells in the organ of Corti.
|Awardees||Award Date||Honours / Awards / Prizes||Category|
|2010-05-15||Best Poster Award: Sox10 mutation affects gliogenesis of the cochleo-vestibular ganglion: 2010 Hong Kong Inter-University Biochemistry Postgraduate Symposium, 15 May 2010||Research Achievement|
|2007-09-01||Small project fund and Top-up fund for supporting the project related to the analysis of the Hoxb1 - Six1 regulatory pathway essential for sensory neurogenesis of the inner ear: The Committee on Research and Conference Grants in the University of Hong Kong||Research Achievement|
|2009-02-01||Postdoctoral Fellow Travel Award was presented by the panel of adjudications at the 2009 MidWinter Meeting: Association for Research in Otolaryngology ARO, USA||Research Achievement|
|2008-07-01||Travel award was presented by Gordon Research Conference Auditory System 2008: Gordon Research Conference organization||Research Achievement|
|2007-07-01||The University of Hong Kong/China Medical Board Grant for supporting the overseas training in the Professor Bernd Fritzsch in the University of Iowa, USA.: The Faculty of Medicine in the University of Hong Kong||Research Achievement|
|2007-07-01||Fellowship was presented by the 6th Molecular Biology of Hearing and Deafness Conference 2007: Welcome Trust||Research Achievement|
|2013-06-15||Best Poster Award: The roles of Irx3 and Irx5 in mammalian inner ear development: 2013 Hong Kong Inter-University Biochemistry Postgraduate Symposium, CUHK, Hong Kong, June 15, 2013.||Research Achievement|
|2005-12-01||Outstanding presentation award was presented by the panel of adjudications at the 10th Research Postgraduate Symposium: The Faculty of Medicine in the University of Hong Kong||Research Achievement|
|2011-06-11||Best Poster Award: Sox10 is essential for inner ear gangliogenesis.: 2011 Hong Kong Inter-University Biochemistry Postgraduate Symposium, June 11, 2011||Research Achievement|
|2016-06-01||Best Poster Award: Sufu regulates the cell cycle exit and differentiation of cochlear hair cell progenitors: 2016 Hong Kong Inter-University Postgraduate Symposium in Biochemical Sciences, CUHK, Hong Kong, June 11, 2016.||Research Achievement|
|2010-06-20||Best Poster Award: Sox10 mutation affects cochleo-vestibular ganglion gliogenesis: 43rd Annual Meeting for the Japanese Society of Developmental Biologists, Kyoto, Japan, 20-23 June, 2010.||Research Achievement|
|2012-06-09||Best Poster Award: Sox10 is essential for neural crest invasion in cochleo-vastibular ganglion.: 2012 Inter-University Postgraduate Symposium on Life Science, HKUST, Hong Kong, 9 June 2012.||Research Achievement|
|2014-12-01||The Appreciation of Research Achievement Honor certificate: The Committee of Science and Technology Innovation of Shenzhen, China||Research Achievement|
|2014-06-01||Best Poster Award: Sufu and Gli3 repressor mediate the temporal basal-to-apical progression of hair cell differentiation in mammalian cochleae: 2014 Hong Kong Inter-University Biochemistry Postgraduate Symposium, HKUST, June 14, 2014||Research Achievement|
|Term Period||Position||Professional Societies|
|02/2008-03/2017||Regular Membership||Association for Research in Otolaryngology ARO|
|01/2011-03/2017||Professional Membership||New York Academy of Sciences|
|01/2013-03/2017||Academic Membership||Hong Kong Society of Developmental Biology|
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