Applications of Murine Monoclonal Antibodies to the Salmonella Serogroup specific O and Vi antigens in the Clinical Microbiology Laboratory
Dr Tsang, Raymond Shiu Wah (Principal investigator)
HKU Project Code
Strategic Research Fund (UPGC)
To raise murine monoclonal antibodies specific for the lipopolysaccharides of serogroups B, C1, and E Salmonella; together with the murine monoclonal antibodies already produced and characterized to be specific for the Vi and O antigens of serogroups A, C2, and D Salmonella, to investigate if the murine monoclonal antibodies to the Salmonella O factors and Vi antigen can be used to replace the conventional polyclonal O factor and Vi specific antisera for the seroplogical typing of Salmonella; to study the possibility of applying these murine monclonal antibodies to detect Salmonella in selective enrichment broth cultures; to study the feasibility of applying these murine monoclonal antibodies to detect Salmonella directly in clinical specimens or food products.