Transfer of foreign genes encoding for phosphorylcholine-specific antibodies to CBA/N mice at the pro-nuclear embryo stage to study the expression of these genes and the role of their proteins in protection against pneumococcal infection


Grant Data
Project Title
Transfer of foreign genes encoding for phosphorylcholine-specific antibodies to CBA/N mice at the pro-nuclear embryo stage to study the expression of these genes and the role of their proteins in protection against pneumococcal infection
Principal Investigator
Emeritus Professor Chan, Samuel Ting Hon   (Principal investigator)
Co-Investigator(s)
Dr Lim Pak Leong   (Co-principal investigator)
Start Date
1988-10-06
Completion Date
1993-05-31
Amount
600000
Conference Title
Presentation Title
HKU Project Code
HKU 29/88
Grant Type
University Grants Committee - General Award
Funding Year
1988/1989
Status
Completed
Objectives
To establish transgenic technology by introducing immunoglobulin genes that encode for phosphorylcholine-specific antibodies of the T15 idiotype into CBA/N mice which naturally lack these antibodies; to characterise the transgenic animals by (a) to study the integration, expression and inheritance of the introduced genes in these animals; (b) to determine whether the transgene-coded antibodies in the reconstituted animals are protective against challege with Streptococcus pneumoniae (pneumococcus), including a comparative study of the efficiencies of the different classes (isotypes) of these antibodies in this role.