Transfer of foreign genes encoding for phosphorylcholine-specific antibodies to CBA/N mice at the pro-nuclear embryo stage to study the expression of these genes and the role of their proteins in protection against pneumococcal infection

Emeritus Professor Chan, Samuel Ting Hon   (Principal Investigator (PI))

Dr Lim Pak Leong   (Co-principal investigator)

1988-10-06

600000

Transfer of foreign genes encoding for phosphorylcholine-specific antibodies to CBA/N mice at the pro-nuclear embryo stage to study the expression of these genes and the role of their proteins in protection against pneumococcal infection

null

N/A

HKU 29/88

University Grants Committee - General Award

1988

Completed

To establish transgenic technology by introducing immunoglobulin genes that encode for phosphorylcholine-specific antibodies of the T15 idiotype into CBA/N mice which naturally lack these antibodies; to characterise the transgenic animals by (a) to study the integration, expression and inheritance of the introduced genes in these animals; (b) to determine whether the transgene-coded antibodies in the reconstituted animals are protective against challege with Streptococcus pneumoniae (pneumococcus), including a comparative study of the efficiencies of the different classes (isotypes) of these antibodies in this role.