Regulation of actin organization during fertilization in flowering plants


Grant Data
Project Title
Regulation of actin organization during fertilization in flowering plants
Principal Investigator
Dr Huang, Bingquan   (Principal investigator)
Duration
36
Start Date
1997-09-01
Completion Date
2000-08-31
Amount
718680
Conference Title
Presentation Title
Sponsor
RGC General Research Fund (GRF)
HKU Project Code
HKU 7232/97M
Grant Type
General Research Fund (GRF)
Funding Year
1997/1998
Status
Completed
Objectives
To test interactions between actin and actin-associated proteins by: a) localization of actin, profilin, and actin depolymerizing factor in fixed and living embryo sacs, b) localization of myosin in pollen and embryo sacs, c) examining filament polarity and dynamics of actin coronas. To perturb actin corona function by microinjecting profilin into unfertilized and fertilized embryo sacs. To examine the role of calcium in actin regulation during fertilization by tracing the changes of actin and calci um in living embryo sacs and synergids.