Investigation of photothermally responsive multi-layered graphene oxide coating for controlled release of antibacterial reagents to act against orthopaedic implant-related infection

Professor Yeung, Kelvin Wai Kwok   (Principal Investigator (PI))

Dr Qiu Jiajun   (Co-Investigator)

Professor Liu Xuan Yong   (Co-Investigator)

Dr Kao Richard Yi Tsun   (Co-Investigator)

36

2022-01-01

1377620

Investigation of photothermally responsive multi-layered graphene oxide coating for controlled release of antibacterial reagents to act against orthopaedic implant-related infection

Antibacterial surface, Bacterial infection, Bone-to-implant integration, Graphene oxide, Photodynamic control, Titanium

Others - Medicine, Dentistry and Health

20190422

Health and Medical Research Fund - Full Grant

2020

On-going

Objective: To investigate the multi-layered graphene oxide (GO) coating systems that can manipulate the release of reactive oxygen species (ROS) and antibiotics through photodynamic control. Hypothesis: The new photothermally responsive multi-layered GO coating that enables the sustained and controlled release of ROS and antibiotics can effectively kill the bacteria attached on implant surface and promote bone-to-implant integration. Design and subjects: The samples will be tested against with (1) bacteria including E. coli (gram-negative) and S. aureus (gram-positive) for in vitro antibacterial properties, (2) human mesenchymal stem cells (hMSCs) and rat bone marrow mesenchymal stem cells (rBMSCs) in vitro for cytotoxicity and osteogenic differentiation properties and, (3) the infected rat models for antibacterial properties and bone-to-implant integration capability in vivo. Instruments: Various instruments in cell biology, molecular biology, histology, biological properties and animal surgery will be included. Intervention: The GO-coated Ti implant that enables the controlled release of ROS and antibiotics will be implanted into the infected rat model. Main outcome measures: The antibacterial and biological responses e.g. cytocompatibility, osteogenic differentiation capability, tissue infection and inflammation, bone-to-implant integration of GO-coated Ti implant will be measured. Data analysis: Data on testing groups will be analysed by post-hoc Tukey’s test, Student’s t-test, when the data are normally distributed. Differences will be considered significant when the probability level reaches 95% (p<0.05). Expected results: The antibacterial property and bone-to-implant integration capability induced by photo-thermally responsive GO coating should be superior than that of the control in the infected rat models.